Skip to main content
SpringerLink
Log in

Fructose-1,6-diphosphate counteracts ethanol-stimulated calcium uptake in isolated BHK cells

  • Original Article
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Ethanol increases the uptake of 45Ca by isolated baby hamster kidney (BHK) cells in vitro. The effect is dependent on ethanol and 45Ca++ concentration and on the incubation time. Fructose-1,6-diphosphate (FDP) added at different concentration during the pre-incubation exerts a protective effect through a membrane-stabilizing action which is consistent with its in vivo anti-alcohol activity documented in previous studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hock JB, Taraschi TF: Cellular adaptation to ethanol. TIBS 13: 269–274, 1988

    Google Scholar 

  2. Harris RA, Baxter DM, Mitchell MA, Hitzeman RS: Physical properties and Lipid Composition of Brain Membranes from Ethanol-tolerant-dependent Mice. Mol Pharmacol 25: 401–407, 1984

    Google Scholar 

  3. Hitzemann RS, Schueler HE, Graham C, Brittain J, Kreishman GP: Ethanol-induced changes in neuronal membrane order. An NMR study. Bioch Biophys Acta 859: 180–187, 1986

    Google Scholar 

  4. Chin JH, Goldstein DB: Drug tolerance in Biomembranes: a spin Label Study of the effect of ethanol. Science 196: 684–685, 1977

    Google Scholar 

  5. Taraschi TF, Wu A, Rubin E: Phospholipids Spin Probes Measure the effects of ethanol on the molecular order of liver microsomes. Biochemistry 24: 7096–7101, 1985

    Google Scholar 

  6. Franks NP, Lieb WR: Molecular mechanisms of general anaesthesia. Nature 300: 487–493, 1982

    Google Scholar 

  7. Miller KW: Models of consciousness. Nature 323: 584–585, 1987

    Google Scholar 

  8. Euers AS, Berkozitz BA, D'Avignon DA: Correlation between the anaesthetic effect of halotane and saturable binding in the brain. Nature 328: 157–160, 1987

    Google Scholar 

  9. Galzigna L, Bianchi M, Rizzoli V, Scuri R, Burlina A: Ethanol-metabolizing enzymes and effects of fructose 1,6 diphosphate. Proceedings of the 13th Int Symp on Clinical Enzymology-Venice (1988)

  10. Galzigna L, Rizzoli V, Bianchi M, Rigobello MP, Scuri R: Effect of fructose-1,6-diphosphate in rat myocardial tissue. Cell Biochem & Function 7: 91–96, 1989

    Google Scholar 

  11. Lowry OH, Rosebrough NS, Farr AL, Randall RS: Protein measurement with the Folin reagent. J Biol Chem 193: 265–270, 1951

    CAS  PubMed  Google Scholar 

  12. Swartz MH, Repke DI, Katz AM, Rubin E: Effects of ethanol on Calcium Binding and Calcium uptake by cardiac microsomes. Biochem Pharmacol 23: 2369–2376, 1974

    Google Scholar 

  13. Lazzarino G, Costrini R, Mulier L, Candiani A, Galzigna L: Increase of intraerythrocytic fructose 1,6-diphosphate in the incubation of whole human blood with fructose 1,6-diphosphate. Clin Biochem 17: 42–45, 1984

    Google Scholar 

  14. Manani G, Galzigna L, Costa G, Trambuscio B, Alati GL, Suma V, Giovannoni G, Volpe A, Giron GP: Clinical use of fructose 1,6-diphosphate. Agressologie 18: 207–212, 1977

    Google Scholar 

  15. Gutknecths J, Tosteson DC: Ionic permeability of thin lipid membranes. J Gen Physiol 55: 359-?, 1970

    Google Scholar 

  16. Galzigna L, Rigobello MP: Proton and potassium fluxes in rat red blood cells incubated with sugars phosphate. Experientia 42: 138–139, 1986

    Google Scholar 

  17. Rizzoli V, Galzigna L: When and how to treat acute and chronic hypophosphatemias. JAMA (Italian edition) 10: 687–690, 1987

    Google Scholar 

  18. Cattani C, Costrini R, Lazzarino G, Diana M, Galzigna L: Fructose-1,6-diphosphate counteracts potassium effects on cardiac muscle. Pharm Res Comm 16: 785–793, 1984

    Google Scholar 

  19. Seeman P, Galey WR, Solomon AK: The effect of Anaesthetics (chlorpromazine, ethanol) on erythrocyte permeability to water. Biochim Biophys Acta 211: 365–368, 1970

    Google Scholar 

  20. Borle AB: Control, modulation and regulation of cell calcium. Rev Physiol Biochem Pharmacol 90: 19–153, 1980

    Google Scholar 

  21. Glossmann H, Ferry DR, Lubbecke F, Mewes R, Hofmann F: Calcium channels: direct identification with radioligand binding studies. TIPS 3: 431–437, 1982

    Google Scholar 

  22. Watkins JC: Pharmacological receptors and General Permeability phenomena of cell membranes. J Theoret Biol 9: 37–50, 1965

    Google Scholar 

  23. Vodoz SF, Luisier M, Curvoisier B, Garcia B: Diminution de 1'absorption intestinale de 47Ca dans l'alcoolisme cronique. Schweiz Med Wochensh 107: 1525–1529, 1977

    Google Scholar 

  24. Peng T, Cooper CW, Hunson PL: The hypocalcemic effect of ethyl alcohol in rats and dogs. Endocrinology 91: 586–593, 1972

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Chemistry, University of Padova, Italy

    Valeria Rizzoli, Graziella Battilana & Lauro Galzigna

Authors
  1. Valeria Rizzoli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Graziella Battilana
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lauro Galzigna
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rizzoli, V., Battilana, G. & Galzigna, L. Fructose-1,6-diphosphate counteracts ethanol-stimulated calcium uptake in isolated BHK cells. Mol Cell Biochem 95, 89–93 (1990). https://doi.org/10.1007/BF00219534

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00219534

Key words

Search

Navigation