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Adverse effects of hyperbaric oxygen on [3H]uridine incorporation and uridine kinase activity in B104 rat neuroblastoma cells

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Abstract

The effects of hyperbaric oxygen on uracil nucleotide metabolism in B104 rat neuroblastoma cells were investigated. Cells exposed to 10 atm O2 for 4 h incorporated markedly less [3H]uridine into the acid-soluble fraction and RNA compared to cells kept in ambient air. The acid-soluble fraction of the oxygen-treated cells contained less total [3H]uridine phosphates ([3H]UMP + [3H]UDP + [3H]UTP) than air-treated cells. Uridine kinase activity, assayed in cytosolic extracts from cells exposed to 10 atm O2 for 4 h, was decreased by 46% compared to the air controls. The reduced enzyme activity which appears to account for the depressed [3H]uridine incorporation, may contribute to the lethal effects of oxygen in these cells.

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Abbreviations

DMEM:

Dulbecco's Modified Eagle's Medium

Hepes:

4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid

References

  1. Rueckert RR, Mueller GC: Effect of oxygen tension on the HeLa cell growth. Cancer Res 20: 944–949, 1960

    Google Scholar 

  2. Brosemer RW, Rutter WJ: The effect of oxygen tension on the growth and metabolism of a mammalian cell. Exp Cell Res 25: 101–113, 1961

    Google Scholar 

  3. Balin AK, Goodman DBP, Rasmussen H, Cristofalo VJ: The effect of oxygen tension on the growth and metabolism of WI-38 cells. J Cell Physiol 89: 235–250, 1976

    Google Scholar 

  4. Drew RM, Painter RB, Feinendegen LE: Oxygen inhibition of nucleic acid synthesis in HeLa S3 cells. Exp Cell Res 36:297–309,1964

    Google Scholar 

  5. Junod AF, Petersen H, Jornot L: Thymidine kinase, thymidylate synthase, and endothelial cell growth under hyperoxia. J Appl Physiol 62: 10–14, 1987

    Google Scholar 

  6. Wittner M, Rosenbaum RM: Inhibition and reversal of intranuclear RNA synthesis by hyperatmosperic oxygen. J Appl Physiol 33: 820–824, 1972

    Google Scholar 

  7. Gendimenico GJ, Schlesinger HR, Ritter MA, Haugaard N: Inhibition of growth and decreased survival of B104 rat neuroblastoma cells after exposure to hyperbaric oxygen. In Vitro 20: 385–390, 1984

    Google Scholar 

  8. Gertz BJ, Haugaard ES: Effect of diabetes and fasting on the uridine triphosphate content and uridine kinase activity of rat cardiac and skeletal muscle. Metabolism 28: 358–362, 1979

    Google Scholar 

  9. Oyama VI, Eagle H: Measurement of cell growth in tissue culture with a phenol reagent (Folin Ciocalteau). Proc Soc Exp Biol Med 91: 305–307, 1956

    Google Scholar 

  10. Randerath K, Randerath E: Ion-exchange chromatography of nucleotides on poly-(ethyleneimine)-cellulose thin layers. J Chromatog 16: 111–125, 1964

    Google Scholar 

  11. Lowry OH, Rosebrough NJ, Farr AL, Randall R: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–273, 1951

    CAS  PubMed  Google Scholar 

  12. Plagemann PGW, Marz R, Wohlhueter RM: Uridine transport in Novikoff rat hepatoma cells and other cell lines and its relationship to uridine phosphorylation and phosphorolysis. J Cell Physiol 97: 49–72, 1978

    Google Scholar 

  13. Levine RL, Hoogenraad NJ, Kretchmer N: A review: biological and clinical aspects of pyrimidine metabolism. Pediatr Res 8: 724–734, 1974

    Google Scholar 

  14. Haugaard N: Cellular mechanisms of oxygen toxicity. Physiol Rev 48: 311–373, 1968

    Google Scholar 

  15. Haugaard N: Oxygen poisoning XI. The relation between inactivation of enzymes by oxygen and essential sulfhydryl groups. J Biol Chem 164: 265–270, 1946

    Google Scholar 

  16. Vesely J, Cihak A, Frantisek S: Enhanced stability of uridine kinase from mouse leukemic cells resistant to 5-azacytidine. Thermal inactivation and effect of p-chloromercuribenzoate. Eur J Biochem 22: 551–556, 1971

    Google Scholar 

  17. Fridovich I: Superoxide radical: an endogenous toxicant. Ann Rev Pharmacol Toxicol 23: 239–257, 1983

    Google Scholar 

  18. Reuvers AP, Greenstock CL, Borsa J, Chapman JD: Studies on the mechanism of chemical radio protection by dimethylsulphoxide. Int J Radiat Biol 24: 533–536, 1973

    Google Scholar 

  19. Kelly FJ: Effect of hyperoxic exposure on protein synthesis in the rat. Biochem J 249: 609–612, 1988

    Google Scholar 

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Author notes

  1. Gerard J. Gendimenico

    Present address: R.W. Johnson Pharmaceutical Research Institute at Ortho Pharmaceutical Corp., 08869, Raritan, NJ, USA

Authors and Affiliations

  1. Department of Pharmacology, University of Pennsylvania, School of Medicine, 19104-6084, Philadelphia, PA, USA

    Gerard J. Gendimenico & Niels Haugaard

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  1. Gerard J. Gendimenico
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  2. Niels Haugaard
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Gendimenico, G.J., Haugaard, N. Adverse effects of hyperbaric oxygen on [3H]uridine incorporation and uridine kinase activity in B104 rat neuroblastoma cells. Mol Cell Biochem 95, 71–76 (1990). https://doi.org/10.1007/BF00219532

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  • DOI: https://doi.org/10.1007/BF00219532

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