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Roles of proteins in cation/membrane interactions of isolated rat cardiac sarcolemmal vesicles

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Abstract

To ascertain the roles of the membrane proteins in cation/sarcolemmal membrane binding, isolated rat cardiac sarcolemmal vesicles were extensively treated with Protease (S. aureus strain V.8). SDS-gel electrophoresis, protein and phosphate analysis confirmed that at least 20–22% of the protein, but none of the phospholipid, was solubilized by this procedure, and that the remaining membrane proteins were extensively hydrolyzed into small fragments. The cation binding properties of the treated vesicles were then examined by analyzing their aggregation behavior. The results demonstrate that this procedure had no effect on the selectivity series for di- and trivalent cation binding, or the divalent cation-induced aggregation behavior of the sarcolemmal vesicles at different pHs, indicating that proteins are probably not involved in these interactions and cannot be the low affinity cation binding sites previously observed [21, 22]. It did, however, change the pH at which protons induced sarcolemmal vesicle aggregation, suggesting a possible role for proteins in these processes. Protease treatment also modified the effects of fluorescamine labelling on divalent cation-induced vesicle aggregation, indicating that the NH, groups being labelled with fluorescamine are located on the sarcolemmal proteins. Together, these results support the hypothesis that di- and trivalent cation binding to the sarcolemmal membrane is largely determined by lipid/lipid and/or lipid/carbohydrate interactions within the plane of the sarcolemmal membrane, and that membrane proteins may exert an influence on these interactions, but only under very specialized conditions.

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Abbreviations

MES:

2-(N-morpholino)ethanesulfonic acid

MOPS:

3-(N-morpholino) propanesulfonic acid

HEPES:

N-2-Hydroxyethylpiperizine-N′-2- ethanesulfonic acid

CHES:

2(N-Cyclohexylamino) ethanesulfonic acid

DTT:

DL-Dithiothreitol

PMSF:

Phenylmethyl-sulfonyl fluoride

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Authors and Affiliations

  1. Department of Physiology and the Cardiovascular Research Laboratories, University of California, Los Angeles

    Kenneth S. Leonards

  2. Center for Health Sciences, School of Medicine, 90024-1760, Los Angeles, California, USA

    Kenneth S. Leonards

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  1. Kenneth S. Leonards
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Leonards, K.S. Roles of proteins in cation/membrane interactions of isolated rat cardiac sarcolemmal vesicles. Mol Cell Biochem 95, 31–42 (1990). https://doi.org/10.1007/BF00219527

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  • DOI: https://doi.org/10.1007/BF00219527

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