Abstract
Activation of Streptococcus mitis (ATTC 9811) arginine aminopeptidase resulted in removal of the metal(s) from the enzyme molecule, and the action of the heavy metal ion in the inactivation process was shown to involve formation of a chelate complex between the enzyme molecule and metal or oxidation of functional group(s) on the enzyme surface. The enzyme also underwent activation by bovine serum albumin, amino acids, phosphate, and citric acid, which are probable physiological chelators.
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Hirakoa BY, Fukasawa K, Harada M: Purification and characterization of two novel arginine aminopeptidases from Steptococcus mitis ATCC 9811. J Biochem 94:1201–1208, 1983.
Hiraoka BY: Immunochemical properties and intracellular localization of two molecular forms of arginine aminopeptidase in Streptococcus mitis ATCC 9811. Biochim Biophys Acta 841:166–172, 1985.
Hiraoka BY, Mogi M, Fukasawa K, Harada M: Coordinate repression of arginine aminopeptidase and three enzymes of the arginine deiminase pathway in Streptococcus mitis. Biochem Int 12: 881–887, 1986.
Hiraoka BY, Fukasawa K, Harada M: Regulation of the activity of Streptococcus mitis arginine aminopeptidase with saliva. Jpn J Oral Biol 25(Suppl):128, 1983 (in Japanese).
Yanagisawa M, Hasegawa H, Ichiyama A: Tryptophan hydroxylase from mouse mastocytoma P-815. Reversible activation by ethylenediaminetetraacetate. J Biochem 92:449–456, 1982.
Hartree EF: Determination of protein: A modification of the Lowry method that gives a linear photometric responce. Analyt Biochem 48: 422–427, 1972.
Porath J, Carlsson J, Olsson I, Belfrage G: Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258:598–599, 1975.
Kronman MJ, Bratcher SC: An experimental artifact in the use of chelating metal ion buffers. J Biol Chem 258:5707–5709, 1983.
Naqui A: What does I 50 mean? Biochem J 215:429–430, 1983.
Bradshaw RA, Shearer WT, Gurd FRN: Sites of binding of copper (II) ion by peptide (1–24) of bovine serum albumin. J Biol Chem 243:3817–3825, 1968.
Lal H: Hydrogen ion equilibria and the interaction of Cu′′ and Co′′ with bovine serum albumin. J Am Chem Soc 81:844–848, 1959.
Kawata S, Takayama S, Ninomiya K, Makisumi S: Porcine liver aminopeptidase B: Substrate specificity and inhibition by amino acids. J Biochem 88:1601–1605, 1980.
Hasegawa H, Kaufman S: Spontaneous activation of phenylalanine hydroxylase in rat liver extracts. J Biol Chem 257:3084–3089, 1982.
Smarrelli J Jr, Campbell WH: Heavy metal inactivation and chelators stimulation of higher plant nitrate reductase. Biochim Biophys Acta 742:435–445, 1983.
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Hiraoka, B.Y., Fukasawa, K. & Harada, M. Metal ion inactivation and chelator stimulation of Streptococcus mitis arginine aminopeptidase. Mol Cell Biochem 73, 111–115 (1987). https://doi.org/10.1007/BF00219425
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DOI: https://doi.org/10.1007/BF00219425