Skip to main content
SpringerLink
Log in

Evidence for calcium enhanced phosphorylation of pyruvate kinase by pancreatic islets

  • Original Articles
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Summary

Pancreatic islet cytosol contains a calcium-calmodulin dependent protein kinase that can mediate the phosphorylation of an endogenous protein that has an Mr of 57 000, as well as exogenous muscle pyruvate kinase (subunit Mr, 57000). EGTA and trifluoperazine decreased the phosphorylation. Alkaline inactivation of pyruvate kinase made it a better substrate for the kinase. As in rat islet cytosol, rabbit islet cytosol catalyzed the phosphorylation of a 57 000 Mr protein in the presence of calcium and calmodulin. This phosphoprotein was immunoprecipitated with anti-pyruvate kinase antibody. This is consistent with the idea that the 57 000 Mr phosphoprotein in islet cytosol is the subunit of pyruvate kinase. The paper following this paper shows that the kinetic and immunologic properties of the islet pyruvae kinase indicate it is the M2 isoenzyme and that its phosphorylation does not affect its catalytic activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Schubart UK, Fleischer N, Erlichman J: J Biol Chem 255:11063–11066, 1980.

    Google Scholar 

  2. Harrison DE, Ashcroft, SJH: Biochim Biophys Acta 714:313–319, 1982.

    Google Scholar 

  3. Landt M, McDaniel ML, Bry CG, Kotogal N, Colca JR, Lacy PE, McDonald MJ: Arch Biochem Biophys 213:148–154, 1982.

    Google Scholar 

  4. MacDonald MJ, Kowluru A, Chang C-M, Mertz RJ. In: Lennon DLF, Stratman FW, Zahlten RN (eds), Biochemistry of Metabolic Processes. Elsevier, Amsterdam, 1982, pp 165–178.

    Google Scholar 

  5. Kowluru A, MacDonald MJ: Biochem Biophys Res Commun 118:797–804, 1984.

    Google Scholar 

  6. Kowluru A, MacDonald MJ: Arch Biochem Biophys 231:320–327, 1984.

    Google Scholar 

  7. MacDonald MJ, Chang C-M: Molec and Cell Bioch 68:115–120, 1985.

    Google Scholar 

  8. Eigenbrodt E, Mostafa A-FM, Schoner W: Hoppe-Seyler's Z Physiol chem 358:1047–1055, 1977.

    Google Scholar 

  9. Eigenbrodt E, Schoner W.: Hoppe-Seyler's Z Physiol Chem 358:1033–1046, 1977.

    Google Scholar 

  10. Glossman H, Presek P, Eigenbrodt E: Molecular and Cellular Endocrinology 23:49–63, 1981.

    Google Scholar 

  11. Nakashima K, Fiyii S, Kaku K, Kaneko T: Biochem Biophys Res Commun 104:285–289, 1982.

    Google Scholar 

  12. Guderly H, Fournier P: Fed Proc 41:1679 (abstract), 1982.

    Google Scholar 

  13. Lacy PE, Kostianovsky M: Diabetes 16:35–39, 1967.

    Google Scholar 

  14. Sharp DW, Kemp CB, Knight MJ, Ballinger WF, Lacy PE: Transplantation 16:686–689, 1973.

    Google Scholar 

  15. MacDonald MJ: J Biol Chem 256:8287–8290, 1981.

    Google Scholar 

  16. MacDonald MJ: Arch Biochem Biophys 213:643–649, 1982.

    Google Scholar 

  17. MacDonald MJ, Kowluru A: Diabetes 31:566–570, 1982.

    Google Scholar 

  18. Portzehl H, Caldwell PC, Ruegg JC: Biochem Biophys Acta 79:581–591, 1964.

    Google Scholar 

  19. Scarpa A, Brinley FJ, Dubyak G: Biochemistry 17:1378–1385, 1978.

    Google Scholar 

  20. Laemmli UK: Nature 227:680–685, 1970.

    PubMed  Google Scholar 

  21. Swanstrom R, Shank PR: Anal Biochem 86:184–192, 1978.

    Google Scholar 

  22. Montague W, Howell SL: Biochem J 129:551–560, 1972.

    Google Scholar 

  23. Schubart UK, Rosen OM: Molecular and Cellular Endocrinology 4:167–182, 1976.

    Google Scholar 

  24. Sugden MC, Ashcroft SJH, Augden PH: Biochem J 180: 219–229, 1979.

    Google Scholar 

  25. Hoffman F, Beavo JA, Bechtel PJ, Krebs EG: J Biol Chem 250:7795–7801, 1975.

    Google Scholar 

  26. Humble E, Berglund L, Titanji V, Llungstrom O, Edlund B, Zetterqvist O, Engstrom L: Biochem Biophys Res Commun 66:614–621, 1975.

    Google Scholar 

  27. Corbin JD, Reimann EM: Methods Enzymol 38:287–290, 1974.

    Google Scholar 

  28. Sugden MC, Christie MR, Ashcroft SJH: FEBS Lett 105:95–100, 1979.

    Google Scholar 

  29. Valverde I, Sener A, Lebrun P, Herchuelz A, Malaisse WJ: Endocrinology 108:1305–1312, 1980.

    Google Scholar 

  30. Sugden MC, Ashcroft SJH: Diabetologia 15:173–180, 1978.

    Google Scholar 

  31. Sugden MC, Ashcroft SJH: Diabetologia 13:481–486, 1977.

    Google Scholar 

  32. Hedeskov CJ, Capito K: Hormone and Metabolic Res 10:8–13, 1980.

    Google Scholar 

  33. MacDonald MJ, Chang C-M: Diabetes 34:246–250, 1985.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. University of Wisconsin Medical School, Room 3459, Medical Sciences Center, 1300 University Avenue, 53706, Madison, Wisconsin, USA

    Michael J. MacDonald & Anjaneyulu Kowluru

Authors
  1. Michael J. MacDonald
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anjaneyulu Kowluru
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

MacDonald, M.J., Kowluru, A. Evidence for calcium enhanced phosphorylation of pyruvate kinase by pancreatic islets. Mol Cell Biochem 68, 107–114 (1985). https://doi.org/10.1007/BF00219374

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00219374

Keywords

Search

Navigation