Summary
The relative density of adrenergic and non-adrenergic nerves in the hepatic portal vein of the rabbit has been determined ultrastructurally. Adrenergic nerves were visualised with the modified chromaffin procedure of Tranzer and Richards (1976). Nearly equal numbers of adrenergic and non-adrenergic nerve profiles were found, indicating a much greater density of innervation by non-adrenergic nerves than that described by Burnstock et al. (1979) using light microscopic histochemical methods. These results imply that part of the argument used by Burnstock et al. (1979) to support “purinergic” transmission in rabbit portal vein is probably invalid.
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References
Ålund M Oison C (1979) Quinacrine affinity of endocrine cell systems containing dense core vesicles as visualized by fluorescence microscopy. Cell Tissue Res 204:171–186
Burnstock G (1972) Purinergic nerves. Pharmacol Rev 24:509–581
Burnstock G, Crowe R, Wong HK (1979) Comparative pharmacological and histochemical evidence for purinergic inhibitory innervation of the portal vein of the rabbit, but not guinea-pig. Br J Pharmacol 65:377–388
Campbell G, Gibbins IL (1979) Non-adrenergic, non-cholinergic transmission in the autonomic nervous system: purinergic nerves. In: Kalsner S (ed) Trends in autonomic pharmacology, vol I. Urban and Schwarzenberg, Baltimore Munich, pp 103–144
Furness JB, McLean JR, Burnstock G (1970) Distribution of adrenergic nerves and changes in neuromuscular transmission in the mouse vas deferens during post-natal development. Dev Biol 21:491–505
Gibbins IL (1980) Ultrastructural characterization of peripheral non-adrenergic autonomic nerves. Micron 11:453–454
Hökfelt T, Johansson O, Ljungdahl A, Lundberg JM, Schultzberg M (1980) Peptidergic nerves. Nature 284:515–521
Holman ME, Kasby CB, Suthers MB, Wilson JAF (1968) Some properties of the smooth muscle of rabbit portal vein. J Physiol 196:111–132
Hughes J, Vane JR (1967) An analysis of the responses of the isolated portal vein of the rabbit to electrical stimulation and to drugs. Br J Pharmacol Chemother 30:46–66
Hughes J, Vane JR (1970) Relaxations of the isolated portal vein of the rabbit induced by nicotine and electrical stimulation. Br J Pharmacol 39:476–489
Komuro T, Burnstock G (1980) The fine structure of smooth muscle cells and their relationship to connective tissue in the rabbit portal vein. Cell Tissue Res 210:257–267
Oison L, Ålund M, Norberg K-A (1976) Fluorescence-microscopical demonstration of a population of gastrointestinal nerve fibres with a selective affinity for quinacrine. Cell Tissue Res 171:407–423
Tranzer JP, Richards JG (1976) Ultrastructural cytochemistry of biogenic amines in nervous tissue: methodologic improvements. J Histochem Cytochem 24:1178–1193
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Gibbins, I.L. The innervation of the hepatic portal vein in the rabbit: Ultrastructural evidence against “purinergic” neurotransmission. Cell Tissue Res. 217, 563–567 (1981). https://doi.org/10.1007/BF00219364
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DOI: https://doi.org/10.1007/BF00219364