Summary
The ultrastructural immunocytochemical peroxidase-antiperoxidase technique used on serial sections reveals that certain corticotropic cells contain both ACTH and FSHβ, but not LHβ (ACTH/FSH cells). To determine the specificity of the anti-FSHβ staining in these cells, immunocytochemical absorption experiments were performed. The results indicate that (1) anti-FSHβ and -ACTH antisera are bound to different antigens in the corticotropic cell, and (2) anti-FSHβ staining is specific for a FSH-like antigen. In the ACTH/FSH cells both hormones are stored in the same secretory granules, distributed among other granules that contain only ACTH.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Closset J, Hennen G (1978) Porcine follitropin. Isolation and characterization of the native hormone and its α and β subunits. Europ J Biochem 86:105–113
Dacheux F, Dubois MP (1976) Ultrastructural localization of prolactin, growth hormone and luteinizing hormone by immunocytochemical techniques in the bovine pituitary. Cell Tissue Res 174:245–260
Dacheux F (1978) Ultrastructural localization of gonadotrophic hormones in the porcine pituitary using the immunoperoxidase technique. Cell Tissue Res 191:219–232
Dacheux F (1980a) Ultrastructural localization of LH and FSH in the porcine pituitary. In: Jutisz M, McKerns W (eds) Synthesis and release of adenohypophyseal hormones. 187–195
Dacheux F (1980b) Ultrastructural localization of corticotropin, β-lipotropin and α- and β-endorphin in the porcine anterior pituitary. Cell Tissue Res (in press)
Moriarty GC, Garner LL (1977) Immunocytochemical studies of cells in the rat adenohypophysis containing both ACTH and FSH. Nature 265:356–358
Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antiperoxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Vandalem JL, Bodart Ch, Pirens G, Closset J, Jennen G (1979) Development and application of homologous radioimmunoassays for porcine gonadotrophins. J Endocrinol 81:1–10
Zamboni L, de Martino C (1967) Buffered picric acid formaldehyde: a new rapid fixation for electron microscopy. J Cell Biol 35:148A
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dacheux, F. Evidence for FSH-like material in ACTH granules of certain corticotropic cells in the pituitary of the pig. Cell Tissue Res. 217, 497–503 (1981). https://doi.org/10.1007/BF00219360
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00219360