Abstract
The calcium dependence of contraction and NADH flurorescence was investigated in rabbit bladder stimulated with bethanechol or KCl. The absence of calcium in the bathing solution induced a rightward shift in the dose response to bethanechol for both contraction and NADH flurorescence. The contractile response was shifted to a greater degree than the fluorescence response and the maximal response to bethanechol was reduced by 80% for contraction but only 20% for NADH fluorescence. This rightward shift was also induced by the benzothiazepine calcium antagonist diltiazem (200 μM) and again the contractile response was shifted significantly more than the fluorescence response. The combination of zero calcium and 200 μM diltiazem virtually abolished contractions but only inhibited the NADH fluorescence by 65% at maximally effective bethanechol concentrations. Unlike the effect of diltiazem on the response to bethanechol, diltiazem (200 μM) shifted both the contraction and fluorescence curves to the right equally in response to KCl stimulation. These results indicate that a metabolic response to muscarinic stimulation (decreased NADH) can occur in the absence of any observable contractile response. This metabolic response may be due to post receptor signal processing events. For KCl stimulation, the NADH response is probably secondary to and a result of the contractile response.
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Abbreviations
- ATP:
-
Adenosine Triphosphate
- KCl:
-
Potassium Chloride
- HPLC:
-
High Performance Liquid Chromatography
- NADH:
-
reduced nicotinamide Adenine Dinucleotide
- NAD:
-
Oxidized Nicotinamide Adenine Dinucleotide
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Ruggieri, M.R., Wein, A.J., Hypolite, J.A. et al. Dissociation of the metabolic from the contractile response to muscarinic stimulation in the rabbit urinary bladder. Mol Cell Biochem 81, 137–143 (1988). https://doi.org/10.1007/BF00219316
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DOI: https://doi.org/10.1007/BF00219316