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Studies on silk secretion in the trichoptera (F. Limnephilidae)

II. Structure and amino acid composition of the silk

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Summary

The ultrastructure and amino acid composition of the secreted silk of two species of trichopteran larvae, Pycnopsyche guttifer (Walk.) and Neophylax concinnus McL., were investigated. The spinnerets of these two animals were also examined by scanning electron microscopy. The silk consists of double-stranded, flat ribbons (1–4 μ wide), composed of bundles of 15–25 Å filaments. There are two components of the silk: the fiber proper and a surrounding coat thought to be a silk “gum”. Only the outer coat is positive to the EM PATP technique of Thiery (1967), which indicated the presence of neutral sugars. Amino acid analyses of Pycnopsyche silk show that, like other silks, two predominant amino acids are glycine and serine. Arginine, unexpectedly, is the third most abundant and there are a large number of basic and long side-chain amino acids. X-ray diffraction studies of the silk indicate that it has a less crystalline, more amorphous structure than that of other silks.

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Authors and Affiliations

  1. Department of Biological Sciences, State University of New York, Albany, New York, USA

    Maryann S. Engster

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  1. Maryann S. Engster
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Submitted to the Department of Biological Sciences of the State University of New York at Albany in partial fulfillment of the requirements for the degree of Doctor of Philosophy

Acknowledgements. This study was supported in part by a National Institutes of Health Graduate Student Traineeship grant # GM-02014. The author would like to express sincere gratitude to Dr. Stephen Brown for his encouragement and help during the course of this study. I would also like to thank Dr. Curtis Hemenway and Mr. Douglas Halgren of Dudley Observatory for the use of their scanning electron microscope as well as Dr. Helen Ghiradella and Mr. William Radigan for help with the scanning electron microscopy. I owe special appreciation to Dr. Y. Myer of the Chemistry Department of SUNYA for doing an amino acid analysis of the silk and to Dr. K.M. Rudall of the University of Leeds for doing the X-ray diffraction studies of the silk samples

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Engster, M.S. Studies on silk secretion in the trichoptera (F. Limnephilidae). Cell Tissue Res. 169, 77–92 (1976). https://doi.org/10.1007/BF00219309

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  • DOI: https://doi.org/10.1007/BF00219309

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