Summary
Uterine tissue from neonatal mice was incubated in vitro at 0° C for 1 h in a medium containing 1×10-8 M 3H-estradiol with or without 1×10-4M dibutyryl cyclic AMP. In some incubations the temperature was raised to 37° C for 15 min after incubation in the cold, in others the temperature was kept at 0° C during this 15 min period. The tissue was frozen in liquid propane cooled in liquid nitrogen, sectioned at 2 or 4 microns, and autoradiograms prepared according to the dry-mount procedure. cAMP increases the cellular uptake of 3H-estradiol in uterine tissue. After rising the temperature to 37° C, grains appeared over the nuclei. cAMP at low temperature increased the cellular uptake of 3H-estradiol, but the grains were not associated with the nuclei. In the autoradiograms the grain number above the epithelium was markedly less than above the stroma.
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This work has been supported by grants from the Norwegian Research Council for Science and the Humanities, and from the Norwegian Cancer Society (Landsforeningen mot Kreft)
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Eide, A., Fossberg, T.M. The effect of cyclic adenosine 3′,5′-monophosphate on the uptake of estradiol by the neonatal mouse uterus. Cell Tissue Res. 169, 1–6 (1976). https://doi.org/10.1007/BF00219302
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DOI: https://doi.org/10.1007/BF00219302