Summary
Reflecting chromatophores in the integument of the guppy, Lebistes reticulatus Peters, are of two distinct types, iridophores and leucophores. The iridophores are smaller and fixed, producing a metallic iridescent color. The cytoplasmic organelles involved in the coloration of iridophores are the reflecting platelets, as in the iridophores of other fish and amphibian species on which earlier reports have been made. Spherical granules of pleiomorphic internal structure, quite variable in size but generally 0.2 μm to 1.0 μm in diameter, are also numerous in the iridophores. The nature of these granules remains unknown.
The leucophores are larger, and highly dendritic; their pigment granules are migratory and they exhibit a dull whitish color. Pigment granules of the leucophores are spherical in form, varying from 0.5–0.8 μm in diameter, with a double membrane enclosing the internal fibrous materials. Melamine-treatment of the fish caused degenerative changes in the pigment granules and also the other cytoplasmic organelles of the leucophores, whereas the other kinds of chromatophores, including the iridiophores, remained intact. Some problems in general characterization and classification between these two types of chromatophores were discussed.
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References
Arnott, H.J., Nicol, J.A.C.: Reflection of ratfish skin (Hydrolagus colliei). Canad. J. Zool. 48, 137–151 (1970)
Bagnara, J.T.: Cytology and cytophysiology of non-melanophore pigment cells. Int. Rev. Cytol. 20, 173–205 (1966)
Bagnara, J.T., Ferris, W.: Interrelationships of vertebrate chromatophores. In: Biology of normal and abnormal melanocytes (T. Kawamura, T.B. Fitzpatrick, M. Seiji, eds.), pp. 57–76. Tokyo: Univ. Tokyo Press 1971
Bagnara, J.T., Taylor, J.D., Prota, G.: Color changes, unusual melanosomes, and a new pigment from leaf frogs. Science 182, 1034–1035 (1973)
Best, A.C.G., Nicol, J.A.C.: Reflecting cells of the elasmobranch tapetum lucidum. Contrib. Marine Sci. Univ. Tex. 12, 172–201 (1967)
Coulter, H.D.: Rapid and improved methods for embedding biological tissues in Epon 812 and Araldite 502. J. Ultrastruct. Res. 20, 346–355 (1967)
Dalton, A.L.: A chrome-osmium fixative for electron microscopy. Anat. Rec. 121, 281 (1955)
Elofsson, R., Kauri, T.: The ultrastructure of the chromatophores of Crangon and Pandalus (Crustacea). J. Ultrastruct. Res. 36, 263–270 (1971)
Fries, E.F.B.: Iridescent white reflecting chromatophores (antaugonophores, iridoleucophores) in certain teleost fishes, particularly in Bathygobius. J. Morph. 103, 203–254 (1958)
Hama, T.: Nouvelle démonstration de la coexistence de la drosoptérine et de la purine dans le leucophore de Médaka (Oryzias lalipes, Téléostéen). Compt. R. Soc. Biol. (Paris) 161, 1197–1200 (1967)
Hama, T.: On the coexistence of drosopterin and purine (drosopterinosome) in the leucophore of Oryzias latipes (teleostean fish) and the effect of phenylthiourea and melamine. In: Chemistry and biology of pteridines (K. Iwai, M. Akino, M. Goto, Y. Iwanami, eds.), pp. 391–398. Tokyo: Intern. Acad. Print. Co. 1970
Harris, J.E., Hunt, S.: The fine structure of iridophores in the skin of the Atlantic salmon (Salmo salar L.). Tiss. Cell 5, 479–488 (1973)
Kamei-Takeuchi, I., Eguchi, G., Hama, T.: Ultrastructure of the pteridine pigment granules of the larval xanthophore and leucophore in Oryzias latipes (teleostean fish). Proc. Jap. Acad. 44, 959–963 (1968)
Kawaguti, S., Kamishima, Y.: A supplementary note on the iridophore of the Japanese porgy. Biol. J. Okayama Univ. 12, 57–60 (1966a)
Kawaguti, S., Kamishima, Y.: Electron microscopy on the blue back of a clupeoid fish, Harengula zunasi. Proc. Jap. Acad. 42, 389–393 (1966b)
Kawaguti, S., Kamishima, Y., Sato, K.: Electron microscopic study on the green skin of the tree frog. Biol. J. Okayama Univ. 12, 97–109 (1965)
Kawaguti, S., Takeuchi, T.: Electron microscopy on guanophores of the medaka, Oryzias latipes. Biol. J. Okayama Univ. 14, 55–65 (1968)
Robinson, W.G. Jr., Charlton, J.S.: Microtubules, microfilaments, and pigment movement in the chromatophores of Palaemonetes vulgaris (Crustacea). J. exp. Zool. 186, 279–304 (1973)
Rohlich, S.T.: Fine structural demonstration of ordered arrays of cytoplasmic filaments in vertebrate iridophores. J. Cell Biol. 62, 295–304 (1974)
Rohlich, S.T., Porter, K.R.: Fine structural observations relating to the production of color by the iridophores of a lizard, Anolis carolinensis. J. Cell Biol. 53, 38–52 (1972)
Setoguti, T.: Ultrastructure of guanophores. J. Ultrastruct. Res. 18, 324–332 (1967)
Setoguti, T., Yonemoto, Y., Hagiwara, A.: An electron microscopic study on the guanophore in the iris of the newt, Triturus pyrrhogaster (Boié). Okajimas Fol. Anat. Jap. 46, 253–263 (1969)
Takeuchi, I.K.: Pterinosomes in erythrophores of the guppy, Lebistes reticulatus. Jap. J. Ichthyol. 22, 43–45 (1975a)
Takeuchi, I.K.: Premelanosomes in melanophores of the guppy Lebistes reticulatus Peters. Naturwissenschaften 10, 488–489 (1975b)
Taylor, J.D., Bagnara, J.T.: Melanosomes of the Mexican tree frog Agalychnis dachnicolor. J. Ultrastruct. Res. 29, 323–333 (1969)
Veron, J.E.N., O'Farrell, A.F., Dixon, B.: The fine structure of Odonata chromatophores. Tiss. Cell 6, 613–626 (1974)
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The author wishes to thank Mr. Yoshiro Yamazaki for his assistance in operating the electron microscope, and Dr. Takao Kajishima (Biological Institute, Nagoya University) for his encouragements
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Takeuchi, I.K. Electron microscopy of two types of reflecting chromatophores (iridophores and leucophores) in the guppy, Lebistes reticulatus Peters. Cell Tissue Res. 173, 17–27 (1976). https://doi.org/10.1007/BF00219263
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DOI: https://doi.org/10.1007/BF00219263