Summary
The temporal pattern of the formation and dissolution of vinculin patches during experimental manipulation of the state of actin within the cell was studied. Cytochalasin D-induced retraction and disappearance of stress fibers is followed, with a brief delay, by the dissolution of vinculin-containing patches and the coordinated redistribution of both actin and vinculin into newly formed amorphous aggregates or foci. Recovery from cytochalasin treatment begins with a transformation of these foci into doughnut-shaped assemblies in which actin and vinculin are precisely co-localized. The emergence and growth of filament bundles is paralleled by the appearance of faint vinculin patches that gradually increase in size in parallel with the stress fibers. If stress fibers are stabilized by microinjected rhodamine-phalloidin against stimuli that normally induce a coordinated redistribution of actin and vinculin, also the vinculin patches persist. These observations indicate that treatments influencing the state of actin in the cell have corresponding effects on the stability of vinculin patches and suggest a strong interdependency of actin and vinculin organization.
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Abercrombie M, Heaysman JEM, Pegrum SM (1971) The locomotion of fibroblasts in culture. IV. Electron microscopy of the leading lamella. Exp Cell Res 67:359–367
Avnur Z, Small JV, Geiger B (1983) Actin-dependent association of vinculin with the cytoplasmic aspect of the plasma membrane in cell contact areas. J Cell Biol 96:1622–1630
Blumberg PM (1980) In vitro studies on the mode of aciton of the phorbol esters, potent tumor promoters. CRC Crit Rev Toxicol 8:153–197
Buckley IK, Porter KR (1967) Cytoplasmic fibrils in living cultured cells: A light and electron microscope study. Protoplasma 64:349–380
Burridge K, Connell L (1983) A new protein of adhesion plaques and ruffling membranes. J Cell Biol 97:359–367
Burridge K, Feramisco J (1980) Microinjection and localization of a 130k protein in living fibroblasts: a relationship to actin and fibronectin. Cell 19:587–595
David-Pfeuty T, Singer J (1980) Altered distribution of the cytoskeletal proteins vinculin and alpha actinin in cultured fibroblasts transformed by Rous sarcoma virus. Proc Natl Acad Sci USA 77:6687–6691
Domnina LV, Gelfand VI, Ivanova OY, Leonova EV, Pletjuschkina OY, Vasiliev JM, Gelfand IM (1982) Effects of small doses of cytochalasins on fibroblasts: Preferential changes of active edges and focal contacts. Proc Nal Acad Sci USA 79:7754–5557
Evans RR, Robson RM, Stromer MH (1984) Properties of smooth muscle vinculin. J Biol Chem 259:3916–3924
Füchtbauer A, Jockusch BM, Maruta H, Kilimann MW, Isenberg G (1983) Microfilament organization is disrupted by F-actin capping proteins injected into living tissue culture cells. Nature 304:361–364
Geiger B (1979) A 130k protein from chicken gizzard: Its localization at the termini of microfilament bundles in cultured chicken cells. Cell 18:193–205
Geiger B (1982) Involvement of vinculin in contact-induced cytoskeletal interactions. Cold Spring Harbor Symp Quant Bio 46:671–682
Geiger B (1983) Membrane-cytoskeleton interaction. Biochim Biophys Acta 737:305–341
Greassmann M, Greassmann A (1976) Early simian virus 40-specific RNA contains information for tumor antigen formation and chromatin replication. Proc Natl Acad Sci USA 73:366–370
Hartwig JH, Stossel TP (1979) Cytochalasin B and the structure of actin gels. J Mol Biol 134:539–553
Heath JP, Dunn GA (1978) Cell-to-substratum contacts of chick fibroblasts and their relation to the microfilament system. A correlated interference reflexion and high voltage electron microscope study. J Cell Sci 29:197–225
Jockusch BM, Isenberg G (1981) Interaction of alpha-actinin and vinculin with actin: Opposite effects on filament network formation. Proc Natl Acad Sci USA 78:3005–3009
Kellie S, Holme TC, Bissel MJ (1985) Interaction of tumor promoters with epithelial cells in culture. Exp Cell Res 160:259–274
Lazarides E, Burridge K (1975) Alpha-actinin: immunofluorescent localization of a muscle structural protein in non-muscle cells. Cell 6:289–298
Lehtonen E, Lehto VP, Badley RA, Virtanen I (1983) Formation of vinculin plaques precedes other cytoskeletal changes during retinoic-acid-induced teratocarcinoma cell differentiation. Exp Cell Res 144:191–197
Marchisio PC, Capasso O, Nitsch L, Cancedda R, Giotti E (1984) Cytoskeleton and adhesion patterns of cultures chick embryo chondrocytes during cell spreading and Rous sarcoma virus transformation. Exp Cell Res 151:332–343
Oesch B, Birchmeier W, (1982) New surface component of fibroblast's focal contacts identified by a monoclonal antibody. Cell 31:671–679
Otto JJ (1983) Detection of vinculin-binding proteins with an 1251-vinculin gel overlay technique. J Cell Biol 97:1283–1287
Rogalski AA, Singer SJ (1985) An integral glycoprotein associated with the membrane attachment sites of actin microfilaments. J Cell Biol 101:785–801
Rosenfeld GA, Hou DC, Dingus J, Mega I, Bryan J (1985) Isolation and partial characterization of human platelet vinculin. J Cell Biol 100:669–676
Schliwa M (1982) Action of cytochalasin D on cytoskeletal networks. J Cell Biol 92:79–91
Schliwa M (1986) The Cytoskeleton. An Introductory Survey. Cell Biology Monographs, Vol. 13, Springer, Wien New York
Schliwa M, van Blerkom J (1981) Structural interaction of cytoskeletal components. J Cell Biol 90:222–235
Schliwa M, Nakamura T, Porter KR, Euteneuer U (1984) A tumor promoter induces rapid and coordinated reorganization of actin and vinculin in cultured cells. J Cell Biol 99:1045–1059
Sefton BM, Hunter T, Ball EH, Singer SJ (19) Vinculin: a cytoskeletal target of the tranforming protein of Rous sarcoma virus. Cell 24:165–174
Shriver K, Rohrschneider L (1981) Organization of pp60 src and selected cytoskeletal proteins within adhesion plaques and junctions of rous sarcoma virus-transformed cells. J Cell Biol 89:525–535
Wehland J, Weber K (1981) Actin rearrangement in living cells revealed by microinjection of a fluorescent phalloidin derivative. Eur J Cell Biol 24:176–183
Wehland J, Osborn M, Weber K (1977) Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth. Proc Natl Acad Sci USA 74:5613–5617
Wehland J, Osborn M, Weber K (1980) Phalloidin associates with mircofilaments after mircoinjection into tissue culture cells. Eur J Cell Biol 21:188–194
Wilkins JA, Lin S (1982) High-affinity interaction of vinculin with actin filaments in vitro. Cell 28:83–90
Wilkins JA, Lin S (1986) A re-examination of the interaction of vinculin with actin. J Cell Biol 102:1085–1092
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Schliwa, M., Potter, M. Relationship between the organization of actin bundles and vinculin plaques. Cell Tissue Res. 246, 211–218 (1986). https://doi.org/10.1007/BF00219020
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DOI: https://doi.org/10.1007/BF00219020