Summary
Immunohistochemically, nerve fibers and terminals reacting with anti-N-terminal-specific but not with anti-C-terminal-specific glucagon antiserum were observed in the following rat hypothalamic regions: paraventricular nucleus, supraoptic nucleus, anterior hypothalamus, arcuate nucleus, ventromedial hypothalamic nucleus and median eminence. Few fibers and terminals were demonstrated in the lateral hypothalamic area and dorsomedial hypothalamic nucleus. Radioimmunoassay data indicated that the concentration of gut glucagon-like immunoreactivity was higher in the ventromedial nucleus than in the lateral hypothalamic area. In food-deprived conditions, this concentration increased in both these parts. This was also verified in immunostained preparations in which a marked enhancement of gut glucagon-like immunoreactivity-containing fibers and terminals was observed in many hypothalamic regions. Several immunoreactive cell bodies were found in the ventromedial and arcuate nuclei of starved rats. Both biochemical and morphological data suggest that glucagon-related peptides may act as neurotransmitters or neuromodulators in the hypothalamus and may be involved in the central regulatory mechanism related to feeding behavior and energy metabolism.
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References
Bloom SR, Polak JM (1978) Gut hormone overview. In: Bloom SR (ed) Gut hormones. Churchill Livingstone, Edinburgh London, pp 3–18
Conlon JM, Samson WK, Dobbs RE, Orci L, Unger RH (1979) Glucagon-like polypeptides in canine brain. Diabetes 28:700–702
Dorn A, Bernstein H, Hahn H, Kostmann G, Zieger M (1980) Regional distribution of glucagon-like immunoreactive material in the brain of rats and sand rats. An immunohistochemical investigation. Acta Histochem 66:269–272
Frohman LA, Bernardis LL, Stachura ME (1974) Factors modifying plasma insulin and glucose responses to ventromedial hypothalamic stimulation. Metabolism 23:1047–1056
Gold RM, Jones AP, Sawchenko PE, Kapatos G (1977) Paraventricular area: Critical focus of a longitudinal neurocircuitry mediating food intake. Physiol Behav 18:1111–1119
Hashimoto PH, Hama K (1968) An electron microscope study on protein uptake into brain regions devoid of the blood-brain barrier. Med J Osaka Univ 18:331–346
Hatton TW, Kovacevic N, Dutczak M, Vranic M (1982) Glucagon-like immunoreactants in extracts of the rat hypothalamus. Endocrinology 111:572–577
Imagawa K, Nishino T, Shin S, Uehata S, Hashimura E, Yanaihara C, Yanaihara N (1979) Production of anti-glucagon sera with a C-terminal fragment of pancreatic glucagon. Endocrinol Jpn 26:123–131
Inokuchi A, Oomura Y, Nishimura H (1984) Effect of intracerbroventricularly infused glucagon on feeding behavior. Physiol Behav 33:397–400
Inokuchi A, Oomura Y, Shimizu N, Yamamoto T (1986) Central action of glucagon in the rat hypothalamus. Am J Physiol 250:R120-R126
Leibowitz SP (1978) Identification of catecholamine receptor mechanisms in the perifornical lateral hypothalamus and their role in mediating amphetamine and L-DOPA anorexia. In: Garattini S, Samanin R (eds) Central mechanisms of anorectic drugs. Raven Press, New York, pp 39–82
Loren I, Alumets J, Håkanson R, Sundler F, Thorell J (1979) Gut-type glucagon immunoreactivity in nerves of the rat brain. Histochemistry 61:335–341
Mlekusch W, Paletta B, Truppe W, Paschke E, Grims R (1981) Plasma concentrations of glucose, corticosterone, glucagon and insulin and liver content of metabolic substrates and enzymes during starvation and additional hypoxia in the rat. Horm Metab Res 13:612–614
Oomura Y (1981) Chemosensitive neuron in the hypothalamus related to food intake behavior. Jpn J Pharmacol [Suppl] 31:1–12
Oomura Y (1983) Glucose as a regulator of neuronal activity. In: Szabo AJ (ed) Advances in metabolic disorders. Vol 10. CNS regulation of carbohydrate metabolism. Academic Press, New York, pp 31–65
Oomura Y (1985) Feeding control through bioassay of body chemistry. Jpn J Physiol 35:1–19
Oomura Y, Niijima A (1983) Chemosensitive neurons and neural control of pancreatic secretion. In: Mongola EN (ed) Diabetes 1982. Excerpta Medica, Amsterdam, pp 201–211
Paxinos G, Watson C (1982) The rat brain in stereotaxic coordinates. Academic Press, New York
Sasaki H, Ebitani I, Tominaga M, Yamatani K, Yawata Y, Hara M (1980) Glucagon-like substance in the canine brain. Endocrinol Japan, SRI: 135–140
Shimazu T, Matsushita H, Ishikawa K (1978) Hypothalamic control of liver glycogen metabolism in adult and aged rats. Brain Res 144:343–352
Shimizu N, Oomura Y, Sakata T (1984) Modulation of feeding by endogenous sugar acids acting as hunger or satiety factors. Am J Physiol 246:R542-R550
Tager H, Hohenboken M, Markese J, Dinerstein J (1980) Identification and localization of glucagon-related peptides in ratv brain. Proc Natl Acad Sci USA 77:6229–6233
Tominaga M, Ebitani I, Marubashi S, Kamimura T, Katagiri T, Sasaki H (1981) Species difference of glucagon-like material in the brain. Life Sci 29:1577–1581
Triepel J, Elger KH, Forssmann WG (1982) Glicentin-immunoreactive perikarya and varicosities in the guinea pig central nervous system. Neurosci Lett 30:285–289
Yanaihara N, Nishino T, Kodaira T, Imagawa K, Nishida T, Mihara S, Yanaihara C (1979) Characterization of anti-glucagon sera elicited against a C-terminal fragment of pancreatic glucagon and their use in glucagon radioimmunoassay. In: Baba S, Kaneko T, Yanaihara N (eds) Proinsulin, Insulin, C-Peptide. Excerpta Medica, Amsterdam, pp 426–430
Yanaihara C, Matsumoto T, Nishida T, Uchida T, Kobayashi S, Moody AJ, Orci L, Yanaihara N (1984) Chemical approach to develop glicentin C-terminal specific radioimmunoassay. Biomed Res [Suppl] 5:19–31
Yoshimatsu H, Niijima A, Oomura Y, Yamabe K, Katafuchi T (1984) Effects of hypothalamic lesion on pancreatic autonomic nerve activity in the rat. Brain Res 303:147–152
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Inokuchi, A., Tomida, Y., Yanaihara, C. et al. Glucagon-related peptides in the rat hypothalamus. Cell Tissue Res. 246, 71–75 (1986). https://doi.org/10.1007/BF00219001
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DOI: https://doi.org/10.1007/BF00219001