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Comparative ultrastructure of the zona radiata from eggs of six species of salmonids

Cell and Tissue Research volume 250, pages 513–519 (1987)Cite this article

Summary

The zona radiata from unactivated and activated eggs from chinook salmon (Oncorhynchus tshawytscha), chum salmon (O. kisutch), pink salmon (O. gorbuscha), brown trout (Salmo trutta), rainbow trout (S. gairdneri) and lake trout (Salvelinus namaycush) were examined using scanning and transmission microscopy. The zona radiata in all species examined consisted of an outer adhesive coating, a thin densely staining zona radiata externa with pore canal plugs and a thick, fibrous zona radiata interna with a fibrous network on the inner surface. There was a two layer adhesive coating over the zona radiata externa in all species except pink salmon in which only one layer was observed. There were structural differences among species in the adhesive layer, zona radiata externa and plugs in the pore-canal openings.

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References

  • Bell GR, Hoskins GE, Bagshaw JW (1969) On the structure and enzymatic degradation of the external membrane of the salmon egg. Can J Zool 47:146–148

    Google Scholar 

  • Bond CE (1979) Biology of Fishes. WB Saunders Company Philadelphia, pp 291–301

    Google Scholar 

  • Eddy FB (1974) Osmotic properties of the perivitelline fluid and some properties of the chorion of Atlantic salmon eggs (Salmo salar). J Zool Lond 174:237–243

    Google Scholar 

  • Flugel H (1967) Lichtund elektronenmikroskopische Untersuchungen an Oozyten und Eiern einiger Knochenfische. Z Zellforsch 83:82–116

    Google Scholar 

  • Ginsburg AS (1963) Sperm-egg association and its relationship to the activation of the egg in salmonid fishes. J Embryo Exp Morphol 2 part 1:13–33

    Google Scholar 

  • Graybill JR, Horton HF (1969) Limited fertilization of chinook and coho salmon eggs with cryopreserved sperm. J Fish Res Board Can 16:1400–1404

    Google Scholar 

  • Grierson JP, Neville AC (1981) Helicoidal architecture offish eggshell. Tissue Cell 13:819–830

    Google Scholar 

  • Hagstrom BE, Lonning S (1968) Electron microscopic studies of unfertilized and fertilized eggs from marine teleosts. Sarsia 33:73–80

    Google Scholar 

  • Harvey B, Kelley RN, Ashwood-Smith MJ (1983) Permeability of intact and dechorionated zebra fish embryos to glycerol and dimethyl sulfoxide. Cryobiology 20:432–439

    Google Scholar 

  • Hayat MA (1981) Principles and Techniques of Electron Microscopy. Biological Appications, Volume 1. Baltimore: University Park Press

    Google Scholar 

  • Hurley DA, Fisher KC (1966) The structure and development of the external membrane in young eggs of the brook trout, Salvelinus fontinalis (Mitchill). Can J Zool 44:173–190

    Google Scholar 

  • Ivankov VN, Kurdyayeva VP (1973) Systematic differences and the ecological importance of the membranes in fish eggs. J Ichthol 13:864–873

    Google Scholar 

  • Kanoh Y (1957) Notes on the mechanism of the monospermic fertilization in fish eggs. J Fac Sci Hokkaido Univ Ser VI Zool 13:394–398

    Google Scholar 

  • Kobayashi W (1982) The fine structure and amino acid composition of the envelope of the chum salmon egg. J Fac Sci Hokkaido Univ Ser VI Zool 23:1–12 plus 2 plates

    Google Scholar 

  • Kudo S (1982) Ultrastructure and ultracytochemistry of the fertilization envelope formation in the carp egg. Dev Growth Differ 24:327–339

    Google Scholar 

  • Lonning S (1972) Comparative electron microscopic studies of teleostean eggs with special reference to the chorion. Sarsia 49:41–48

    Google Scholar 

  • Lonning S (1981) Comparative electron microscope studies of the chorion of the fish egg. Rapp P-V Reum Cons Int Explor Mer 178:560–564

    Google Scholar 

  • Lonning S, Davenport J (1980) The swelling egg of the long rough dab, Hippoglossoides platessoides limandoides (Bloch). J Fish Biol 17:359–378

    Google Scholar 

  • Manery JF, Fisher KC, Moore E (1947) Water intake and membrane hardening of fish eggs. Fed Proc 65:163

    Google Scholar 

  • Osanai K (1977) Scanning electron microscopy of the envelopes surrounding the chum-salmon oocytes. Bull Mar Biol St Asamusiii 16:21–25

    Google Scholar 

  • Riehl R (1980) Micropyle of some salmonins and coregonins. Env Biol Fish 5:59–66

    Google Scholar 

  • Riehl R, Schulte E (1977) Vergleichende rasterelektronenmikroskopische Untersuchungen an den Mikropylen ausgewählter Süsswasser-Teleosteer. Arch Fisch Wiss 28:95–107

    Google Scholar 

  • Schmehl MK (1985) Studies on the structural properties and chemistry of the zona radiata of salmoniforms fish ova. Ph.D. Thesis, University of Minnesota, St. Paul, MN

    Google Scholar 

  • Stehr CM, Hawkes JW (1979) The comparative ultrastructure of the egg membrane and associated pore structures in the starry flounder, Platichthys stellatus (Pallas), and pink salmon, Onchorhynchus gorbuscha (Walbaum). Cell Tissue Res 202:347–356

    Google Scholar 

  • Toshimori K, Yasuzumi F (1976) The morphology and the function of the oocyte chorion in the teleost, Plecoglossus altivelis. J Electron Microsc 25:210

    Google Scholar 

  • Yamamoto K (1951) Activation of the egg of the dog-salmon by water and the associated phenomena. Jour Fac Sci Hokkaido Univ Ser VI Zool 10:303–319

    Google Scholar 

  • Young EG, Smith DG (1956) The amino acids in the inchthulokeratin of salmon eggs. J Biol Chem 219:161–164

    Google Scholar 

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Affiliations

  1. Department of Animal Physiology, University of Minnesota, St. Paul, Minnesota, USA

    M. K. Schmehl &  E. F. Graham

Authors
  1. M. K. Schmehl
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  2. E. F. Graham
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Additional information

Scientific Journal Series, Paper No. 14,627, Minnesota Agricultural Experiment Station

Partially funded by Minnesota Sea Grant NA-82-AA 12-000-39, Project RF-12, Minnesota Sea Grant Contribution 168

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Schmehl, M.K., Graham, E.F. Comparative ultrastructure of the zona radiata from eggs of six species of salmonids. Cell Tissue Res. 250, 513–519 (1987). https://doi.org/10.1007/BF00218942

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  • DOI: https://doi.org/10.1007/BF00218942

Key words

  • Oocytes
  • Structural patterns
  • Pore canals
  • Ultrastructure
  • Salmonids
  • Onchorhynchus tshawytscha, O. kisutch, O. gorbuscha, Salmo trutta, S. gairdneri, Salvelinus namaycush