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Cell and Tissue Research

, Volume 256, Issue 2, pp 365–372 | Cite as

Specific adhesion between pheochromocytoma (PC12) cells and adrenal medullary endothelial cells in co-culture

  • Yaffa Mizrachi
  • Peter I. Lelkes
  • Richard L. Ornberg
  • Gertrude Goping
  • Harvey B. Pollard
Article

Summary

Chromaffin cells in the adrenal medulla are found in close proximity to capillary endothelial cells, thereby forming the classical endocrine complex. To examine the possible chemical basis of their interaction in more detail, we have grown bovine adrenal medullary endothelial (BAME) cells in monolayer cultures and added to them pheochromocytoma (PC12) cells, a chromaffin tumor cell line of rats. The PC12 cells were chosen because of the similarities they share with adrenal medullary chromaffin cells. PC12 cells rapidly attached to BAME cells cultures, their rate of adhesion being significantly enhanced over binding of PC12 cells to either uncoated plates or to monolayers of unrelated cell cultures. Consistent with this observation, we noted that the extracellular matrix (ECM) derived from the BAME cells did not enhance PC12 cell adhesion and did not promote neurite sprouting as previously described for ECM derived from corneal endothelial cells. The specific adhesion between PC12 and BAME cells could be abolished by cell surface extracts derived from these two cells but not by extracts derived from unrelated cell types. This activity was heat-labile, sensitive to trypsin and, to a lesser extent, to neuraminidase. We therefore conclude that PC12 cells may interact with BAME cells by specific proteinaceous adhesive factors associated with their plasma membranes. These interactions might represent the formative role of cell-cell contacts in the organization of the developing adrenal gland.

Key words

Adrenal medullary endothelial cells Pheochromocytoma (PC12) cells Co-culture Cell surface extracts Adhesion Cell-cell interactions Bovine Rat 

Abbreviations

BAME

bovine adrenal medullary endothelial cells

DMEM

Dulbecco's modified essential medium

ECM

extracellular matrix

EMEM

Eagle's modified essential medium

FCS

fetal calf serum

PBS

phosphate-buffered saline

PC12

rat pheochromocytoma cells

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Yaffa Mizrachi
    • 1
  • Peter I. Lelkes
    • 1
  • Richard L. Ornberg
    • 1
  • Gertrude Goping
    • 1
  • Harvey B. Pollard
    • 1
  1. 1.Laboratory of Cell Biology and Genetics, NIDDK, National Institutes of HealthBethesdaUSA

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