Summary
Developmental changes of thyrotropin-releasing hormone (TRH)-immunoreactive structures in the brain of mallard embryos were studied by means of immunocytochemistry (PAP technique). The primary antibody was generated against synthetic TRH. Immunoreactive neurons were first detected in the hypothalamus of 14-day-old embryos. By day 20, increasing numbers of immunoreactive perikarya were observed in the paraventricular nucleus, anterior preoptic region and supraoptic region. Immunoreactive fiber projections were seen in the median eminence as early as embryonic day 20; they occurred also in some extrahypothalamic regions (lateral septum, accumbens nucleus). The number and staining intensity of the cell bodies increased up to hatching, and continued to increase during the first week after hatching.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bøhler J, Enzmann F, Folkers K, Bowers CY, Schally AV (1969) The identity of chemical and hormonal properties of the thyrotropin-releasing hormone and pyroglutamyl-histidyl-proline amide. Biochem Biophys Res Commun 37:705–710
Breneman WR, Rathkamp W (1973) Release of thyroid stimulating hormone from chick anterior pituitary glands by thyrotropin releasing hormone (TRH). Biochem Biophys Res Commun 52:189–194
Burgus R, Dunn TF, Desiderio D, Ward DN, Vale W, Guillemin R (1970) Characterization of ovine hypothalamic hypophysiotropic TSH-releasing factor. Nature (Lond) 226:321–325
Daugeras-Bernard N, Lachiver F (1983) Effect of hypophysectomy by partial decapitation and treatment with thiourea on iodine metabolism in the developing chick embryo. J Endocrinol 98:113–119
Erlanger BF, Borek F, Beiser SM, Lieberman S (1957) Steroid-protein conjugates. I. Preparation and characterization of conjugates of bovine serum albumin with testosterone and with corticosterone. J Biol Chem 228:713–727
Faivre-Bauman A, Grouselle D, Nemeskeri A, Tixier-Vidal A (1978) Ontogenesis of thyroliberin in the mouse hypothalamus. Brain Res 154:382–387
Hall TR, Chadwick A, Bolton NJ, Scanes CG (1975) Prolactin release in vitro and in vivo in the pigeon and domestic fowl following administration of synthetic thyrotropin-releasing factor (TRF). Gen Comp Endocrinol 25:298–306
Harvey S, Scanes CG, Chadwick A, Bolton NJ (1978) The effect of thyrotropin-releasing hormone (TRH) and somatostatin (GHRIH) on growth hormone and prolactin secretion in vitro and in vivo in the domestic fowl (Gallus domesticus). Neuroendocrinology 26:249–260
Jackson IMD, Reichlin S (1974) Thyrotropin-releasing hormone (TRH): distribution in hypothalamic and extrahypothalamic brain tissues of mammalian and submammalian chordates. Endocrinology 95:854–862
Józsa R, Korf HW, Csernus V, Mess B (1988) Thyrotropin-releasing hormone (TRH)-immunoreactive structures in the brain of the domestic mallard. Cell Tissue Res 251:441–449
Mess B, Straznicky K (1964) Functional differentiation of the thyroid gland investigated by 131J autoradiography in decapitated chick embryos bearing hypophyseal homografts. Acta Biol Acad Sci Hung 15:77–85
Mess B, Straznicky K (1970) Differentiation and function of the hypophyseal-target organ system in chicken embryos. Stud Biol Hung (Budapest) 9:1–100
Nistico G, Rotiroti D, DeSarro A, Stephenson JD (1978) Behavioral, electrocortical and body temperature effects after intracerebral infusion of the TRH in fowls. Eur J Pharmacol 50:253–260
Pethes Gy, Scanes CG, Rudas P (1979) Effect of synthetic thyrotropin-releasing hormone on the circulating growth hormone concentration in cold and heat stressed ducks. Acta Vet Acad Sci Hung 27:175–177
Proudman JA, Opel H (1981) Effect of food or water restriction on basal and TRH stimulated growth hormone secretion in growing turkey poults. Poult Sci 60:659–662
Scanes CG (1974) Some in vitro effects of synthetic thyrotropin-releasing factor on the secretion of thyroid stimulating hormone from the anterior pituitary gland of the domestic fowl. Neuroendocrinology 15:1–9
Schaffer JM, Brownstein MJ (1980) Ontogeny of TRH-like material in several regions of the rat brain. Brain Res 182:207–210
Stefanini M, DeMartino C, Zamboni L (1967) Fixation of ejaculated spermatozoa for electron microscopy. Nature 216:173–174
Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Thommes RC, Tonetta SA (1979) Hypothalamo-adenohypophyseal-thyroid interrelationships in the chick embryo. II. Effect of thiourea treatment on plasma total thyroxine levels and thyroidal 125J uptake. Gen Comp Endocrinol 37:167–176
Thommes RC, Vieth RL, Lavasseur S (1977) The effects of hypophysectomy by means of surgical decapitation on thyroid function in the developing chick embryo. I. Plasma thyroxine. Gen Comp Endocrinol 31:29–36
Thommes RC, Martens JB, Hopkins WE, Caliendo J, Sorrentino MJ, Woods JE (1983) Hypothalamo-adenohypophyseal-thyroid interrelationships in the chick embryo. IV. Immunocytochemical demonstration of TSH in the hypophyseal pars distalis. Gen Comp Endocrinol 51:434–443
Thommes RC, Williams J, Woods JE (1984) Hypothalamo-adenohypophyseal-thyroid interrelationships in the chick embryo. VI. Mid-gestational adenohypophyseal sensitivity to thyrotropinreleasing hormone. Gen Comp Endocrinol 55:275–279
Thommes RC, Caliendo J, Woods JE (1985) Hypothalamo-adenohypophyseal-thyroid interrelationships in the developing chick embryo. VII. Immunocytochemical demonstration of thyrotropin releasing hormone. Gen Comp Endocrinol 37:1–9
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Józsa, R., Mess, B. & Csernus, V. Ontogenetic development of thyrotropin-releasing hormone (TRH)-immunoreactive structures in the brain of the mallard embryo. Cell Tissue Res. 255, 657–662 (1989). https://doi.org/10.1007/BF00218805
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218805