Summary
In order to identify the cell-type responsible for the production of hypocalcin (the recently isolated hypocalcemic hormone of teleost fish), the corpuscles of Stannius (CS) of trout, flounder and goldfish, were immunocytochemically stained with antisera raised against trout hypocalcin. The secretory granules of the type-1 cells of the CS, considered to be the hypocalcin-producing cells, showed intense immunoreactivity in all species examined. However, in trout and flounder, the secretory granules produced by the type-2 cells, which have been suggested to represent a functionally different cell-type, also showed an intense immunoreactivity. In goldfish, no type-2 cells were observed. We tentatively conclude that type-1 and type-2 cells represent structurally different forms of the same functional cell-type.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aida K, Nishioka RS, Bern HA (1980) Degranulation of the Stannius corpuscles of coho salmon (Oncorhynchus kisutch) in response to ionic changes in vitro. Gen Comp Endocrinol 4:305–313
Bhattacharya TK, Butler DB (1978) Fine structure of the corpuscles of Stannius in the toadfish. J Morphol 155:271–286
Bhattacharya TK, Butler DG, Youson JH (1982) Ultrastructure of the corpuscles of Stannius in the garpike (Lepisosteus platyrhynchus). Gen Comp Endocrinol 46:29–41
Butkus A, Roche PJ, Fernley RT, Haralambides J, Penschow JD, Ryan GB, Trahair JF, Tregear GW, Coghlan JP (1987) Purification and cloning of corpuscles of Stannius protein from Anguilla australis. Mol Cell Endocrinol 54:123–133
Cohen RS, Pang PKT, Clark NB (1975) Ultrastructure of the corpuscles of Stannius of the killifish, Fundulus heteroclitus, and its relation to calcium regulation. Gen Comp Endocrinol 27:413–423
Fenwick JC (1982) Some evidence concerning the nature of the hypocalcemic factor in the Stannius corpuscles. In: Oguro C, Pang PKT (eds) Comparative Endocrinology of Calcium Regulation. Jpn Sci Soc Press, Tokyo, pp 167–172
Flik G, Perry SF (1988) Cortisol stimulates whole body calcium uptake and the branchial calcium pump in freshwater rainbow trout. J Endocrinol 119 (in press)
Fontaine M (1964) Corpuscules de Stannius et régulation ionique (Ca, K, Na) du milieu intérieur de l'anguille (Anguilla anguilla L). CR Acad Sci (Paris) 259:875–878
Hanssen RGJM, Lafeber FPJG, Flik G, Wendelaar Bonga SE (1988) Ionic and total calcium levels in the blood of the European eel (Anguilla anguilla): effects of stanniectomy and replacement therapy. J Exp Biol (in press)
Kaneko T, Kobayashi M, Aida K, Hanyu I (1985) Ultrastructural immunocytochemistry of gonadotrophs in the goldfish pituitary gland. Cell Tissue Res 239:337–342
Kaneko T, Fraser RA, Labedz T, Harvey S, Lafeber FPJG, Pang PKT (1988) Characterization of antisera raised against hypocalcin (Teleocalcin) purified from corpuscles of Stannius of rainbow trout, Salmo gairdneri. Gen Comp Endocrinol 69:238–245
Krishnamurthy VG (1976) Cytophysiology of corpuscles of Stannius. Int Rev Cytol 46:177–246
Krishnamurthy VG, Bern HA (1969) Correlative histological study of the corpuscles of Stannius and the juxtaglomerular cells of teleost fishes. Gen Comp Endocrinol 13:313–335
Lafeber FPJG (1988) Comparison of PTH and hypocalcin in mammalian and teleost bioassays. Doctoral Thesis, University of Nijmegen
Lafeber FPJG, Perry SF (1988) Experimental hypercalcemia induces hypocalcin release and inhibits branchial Ca2+ influx in freshwater trout. Gen Comp Endocrinol 72:136–143
Lafeber FPJG, Hanssen RHGM, Choy YM, Flik G, Herrmann-Erlee MPM, Pang PKT, Wendelaar Bonga SE (1988a) Identification of hypocalcin (teleocalcin) isolated from trout Stannius corpuscles. Gen Comp Endocrinol 69:19–30
Lafeber FPJG, Wendelaar Bonga SE, Flik G, Perry SF (1988b) Hypocalcin from Stannius corpuscles inhibits branchial calcium uptake in trout. Am J Physiol 254:R891-R896
Lopez E, Tisserand-Jochem E, Eyquem A, Milet C, Hillyard C, Lallier F, Vidal B, MacIntyre I (1984) Immunocytochemical detection in eel corpuscles of Stannius of a mammalian parathyroid-like hormone. Gen Comp Endocrinol 53:28–36
Meats M, Ingleton PM, Chester Jones I, Garland HO, Kenyon CJ (1978) Fine structure of corpuscles of Stannius of the trout Salmo gairdneri. Structural changes in response to increased environmental salinity and calcium ions. Gen Comp Endocrinol 36:451–461
Olivereau M, Olivereau J (1978) Prolactin, hypercalcemia and corpuscles of Stannius in seawater eel. Cell Tissue Res 186:81–96
Pang PKT (1973) Endocrine control of calcium metabolism in teleosts. Am Zool 13:775–792
Pang PKT, Schreibman MP, Griffith RW (1973) Pituitary regulation of serum calcium levels in the killifish, Fundulus heteroclitus. Gen Comp Endocrinol 21:536–542
Pang PKT, Pang RK, Sawyer WH (1974) Environmental calcium and the sensitivity of killifish (Fundulus heteroclitus) in bioassays for the hypocalcemic response to Stannius corpuscles from killifish and cod (Gadus morrhua). Endocrinology 94:548–555
Pang PKT, Griffith RW, Maetz J, Pic P (1980) Calcium uptake in fishes. In: Lahlou B (ed) Epithelial Transport in the Lower Vertebrates. Cambridge Univ. Press, Cambridge, pp 121–131
Roth J, Bendayan M, Orci L (1978) Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem 26:1074–1081
Urasa FM, Wendelaar Bonga SE (1985) Stannius corpuscles and plasma calcium levels during the reproductive cycle in the cichlid teleost fish Oreochromis mossambicus. Cell Tissue Res 159:219–227
Urasa FM, Wendelaar Bonga SE (1987) Effects of calcium and phosphate on the corpuscles of Stannius of the teleost fish, Oreochromis mossambicus. Cell Tissue Res 249:681–690
Van Putten LJA, Kiliaan AJ (1988) Immuno electron-microscopic study of the prolactin cells in the pituitary gland of male Wistar rats during aging. Cell Tissue Res 251:353–358
Wagner GF, Hampong M, Park CM, Copp DH (1986) Purification, characterization and bioassay of teleocalcin: a glycoprotein from salmon corpuscles of Stannius. Gen Comp Endocrinol 63:481–491
Wendelaar Bonga SE, Flik G (1982) Prolactin and calcium metabolism in a teleost fish. In: Oguro C and Pang PKT (eds) Comparative endocrinology of calcium regulation. Jpn Sci Soc Press, Tokyo, pp 21–26
Wendelaar Bonga SE, Greven JAA (1975) A second cell type in Stannius bodies of two euryhaline teleost species. Cell Tissue Res 159:287–290
Wendelaar Bonga SE, Pang PKT (1986) Stannius Corpuscles. In: Pang PKT, Schreibman MD (eds) Vertebrate Endocrinology: Fundamental and Biomedical Implications. Vol 1. Academic Press, New York, pp 439–464
Wendelaar Bonga SE, Greven JAA, Veenhuis M (1976) The relation between ionic composition of the environment and secretory activity of the endocrine cell types of Stannius corpuscles in the teleost Gasterosteus aculeatus. Cell Tissue Res 175:297–312
Wendelaar Bonga SE, Van der Mey JCA, Pang PKT (1980) Evidence for two secretory cell types in the Stannius bodies of the teleost Fundulus heteroclitus and Carassius auratus. Cell Tissue Res 212:295–306
Yoshimura F (1986) A new concept of anterior pituitary cell classification in the rat based on both cell differentiation and the secretory cycle. In: Yoshimura F, Gorbman A (eds) Pars distalis of the pituitary gland structure, function and regulation. Elsevier Science Publishers, Amsterdam, pp 59–69
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bonga, S.E.W., Smits, P.W.J.M., Flik, G. et al. Immunocytochemical localization of hypocalcin in the endocrine cells of the corpuscles of Stannius in three teleost species (trout, flounder and goldfish). Cell Tissue Res. 255, 651–656 (1989). https://doi.org/10.1007/BF00218804
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218804