Summary
The choroid plexus consists of the choroidal epithelium, a derivative of the neural tube, and the choroidal stroma, which originates from the embryonic head mesenchyme. This study deals with epithelio-mesenchymal interactions of these two components leading to the formation of the organ. Grafting experiments of the prospective components have been performed using the quail-chicken marker technique. Prospective choroidal epithelium of quail embryos, forced to interact with mesenchyme of the body wall of chicken embryos, gives rise to a choroid plexus showing normal morphogenesis and differentiation. The choroidal epithelium induces the differentiation of organtypical fenestrated capillaries, which are highly permeable to intravenously injected horseradish peroxidase. The choroidal epithelium of the grafts constitutes a blood-cerebrospinal fluid barrier. On top of the choroidal epithelium, there are epiplexus cells displaying a typical ultrastructure. The experimental results show that these cells do not originate from the transplanted neural epithelium. Prospective choroidal stroma of chicken embryos does not exert a choroid plexus-inducing influence upon a quail embryo's neural epithelium isolated from parts of the brain that normally do not develop a choroid plexus. The experiments show that the choroidal epithelial cells are determined at least three days before the first organ anlage is detectable.
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References
Bargmann W, Oksche A, Fix JD, Haymaker W (1982) Meninges, choroid plexus, ependyma, and their reactions. Part I: Histology and functional considerations. In: Haymaker W, Adams RD (eds) Histology and Histopathology of the nervous system. Vol I. Ch.C. Thomas, Springfield, Ill, pp 560–641
Becker NH, Novikoff AB, Zimmerman HM (1967) Fine structure observations of the uptake of intravenously injected peroxidase by the rat choroid plexus. J Histochem Cytochem 15:160–165
Benninghoff A (1985) Makroskopische und mikroskopische Anatomie des Menschen, Bd. 3. In: Zenker W (ed) Nervensystem, Haut und Sinnesorgane. Urban und Schwarzenberg, München Wien Baltimore
Birge WJ (1962) Induced choroid plexus development in the chick metencephalon. J Comp Neurol 118:89–95
Bouldin TW, Krigman MR (1975) Differential permeability of cerebral capillary and choroid plexus to lantanum ion. Brain Res 99:444–448
Brightman MW (1967) Intracerebral movement of proteins injected into the blood and cerebrospinal fluid. Anat Rec 157:219
Brightman MW, Reese TS (1969) Junctions between intimately apposed cell membranes in the vertebrate brain. J Cell Biol 40:648–677
Cancilla PA, DeBault LE (1983) Neutral amino acid transport properties of cerebral endothelial cells in vitro. J Neuropathol Exp Neurol 42:191–199
Carpenter SJ, McCarthy LE, Borison HL (1970) Electron microscopic study on the epiplexus (Kolmer) cells of the cat choroid plexus. Z Zellforsch 110:471–486
Castel M, Sahar A, Erlij D (1974) The movement of lanthanum across diffusion barriers in the choroid plexus of the cat. Brain Res 67:178–184
Cohen H, Davies S (1938) The development of the CSF spaces and the choroid plexuses in the chick. J Anat 72:23–53
De Bault LE, Cancilla PA (1980) γ-Glutamyl transpeptidase in isolated brain endothelial cells: Induction by glial cells in vitro. Science 207:653–655
Dermietzel R (1976) Die Darstellung eines komplexen Systems endothelialer und perivaskulärer Membrankontakte im Plexus chorioideus. Verh Anat Ges 70:461–469
El-Gammal S (1983) Regional surface changes during the development of the telencephalic choroid plexus in the chick. Cell Tissue Res 231:251–263
Feulgen R, Rossenbeck H (1924) Mikroskopisch-chemischer Nachweis einer Nucleinsäure vom Typ der Thymonucleinsäure und die darauf beruhende elektive Färbung von Zellkernen in mikroskopischen Präparaten. Hoppe Seylers Z Physiol Chem 135:203–252
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Exp Zool 80:347–389
Kahle W, Leonhardt H, Platzer W (1986) Taschenatlas der Anatomie. III: Nervensystem und Sinnesorgane, Georg Thieme Verlag, Stuttgart
Kappers JA (1953) Beitrag zur experimentellen Untersuchung von Funktion und Herkunft der Kolmerschen Zellen des Plexus choroideus beim Axolotl und Meerschweinchen. Z Anat Entwickl Gesch 117:1–19
Kolmer WW (1921) Über eine eigenartige Beziehung von Wanderzellen zu dem Chorioideal-plexus des Gehirns der Wirbeltiere. Anat Anz 54:15–19
LeDouarin NM (1969) Particularités du noyau interphasique chez la caille japonaise (Coturnix coturnix japonica). Utilisation de ces particularités comme “marquage biologique” dans les recherches sur les interactions tissulaires et les migrationes cellulaires au cours de l'ontogenèse. Bull Biol Fr Belg 103:435–452
Leonhardt H (1980) Ependym und circumventrikuläre Organe. In: Oksche A, Vollrath L (eds) Handbuch der mikroskopischen Anatomie des Menschen, Bd. IV/10. Springer, Berlin Heidelberg New York, pp 177–666
Meller K, Wechsler W (1965) Elektronenmikroskopische Untersuchung der Entwicklung der telencephalen Plexus chorioides des Huhnes. Z Zellforsch 65:420–444
Meller K, Wagner HH, Breipohl W (1969) Das Verhalten trypsinisierter Plexus-chorioideus-Zellen in Gewebekultur. Z Zellforsch 97:392–402
Milhorat TH, Davis DA, Lloyd BT Jr (1973) Two morphologically distinct blood-brain barriers preventing entry of cytochrome c into cerebrospinal fluid. Science 180:76–78
Netsky MG, Shuangshoti S (1970) Studies on the choroid plexus. Neurosci Res 3:131–173
Pardanaud L, Altmann C, Kitos P, Dieterlen-Lièvre F, Buck CA (1987) Vasculogenesis in the early quail blastodisc as studied with a monoclonal antibody recognizing endothelial cells. Development 100:339–349
Reese TS, Karnovsky MJ (1967) Fine structural localisation of a blood brain barrier to exogenous peroxidase. J Cell Biol 34:207–217
Schmid H (1929) Anatomischer Bau und Entwicklung der Plexus chorioidei in der Wirbeltierreihe und beim Menschen. Z Mikrosk Anat Forsch 16:413–498
Schwarze EW (1975) The origin of (Kolmer's) epiplexus cells. Histochemistry 44:103–104
Soenarjo DVM (1972) Growth and development of the choroid plexus of the domestic fowl (Gallus domesticus). Dissertation; University of Kentucky, Lexington, Kentucky
Stewart PA, Wiley MJ (1981) Developing nervous tissue induces formation of blood-brain barrier characteristics in invading endothelial cells. Dev Biol 84:183–192
Sturrock RR (1978) A developmental study of epiplexus cells and supraependymal cells and their possible relationship to microglia. Neuropathol Appl Neurobiol 4:307–322
Tennyson VM, Pappas GD (1964) Fine structure of the developing telencephalic and myelencephalic choroid plexus in the rabbit. J Comp Neurol 123:379–411
Wakai S, Hirokawa N (1981) Development of blood-cerebrospinal fluid barrier to horseradish peroxidase in the avian choroidal epithelium. Cell Tissue Res 214:271–278
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This work was supported by the Deutsche Forschungsgemeinschaft (grant Ch 44/7-1)
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Wilting, J., Christ, B. An experimental and ultrastructural study on the development of the avian choroid plexus. Cell Tissue Res. 255, 487–494 (1989). https://doi.org/10.1007/BF00218783
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DOI: https://doi.org/10.1007/BF00218783