Summary
The distribution of nerves showing neuropeptide Y (NPY)-like immunoreactivity in the cardiovascular system of elasmobranchs and teleosts has been investigated. Two species of teleosts, the rainbow trout (Salmo gairdneri) and the Atlantic cod (Gadus morhua), and three species of elasmobranchs, the spiny dogfish (Squalus acanthias), the little skate (Raja erinacea) and the starry ray (Raja radiata), were used in this study. An innervation of the cardiovascular system by an NPY-like substance was found only in the two species of Raja. A rich innervation was encountered in these skates, with the highest density of fibres in the wall of the ventricle, the conus arteriosus, the coeliac artery and smaller mesenterial vessels. In the vessels, the fibres formed a plexus at the adventitio-mediol border. Few fibres were found in the walls of the dorsal aorta, the sinus venosus and the atrium, and no fibres were observed in the walls of the ventral aorta. Falck-Hillarp fluorescence histochemistry showed the presence of a rich innervation of arteries and arterioles of the gut by catecholamine-containing nerve fibres.
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Allen JM, Bircham PMM, Edwards AV, Tatemoto K, Bloom SR (1983) Neuropeptide Y (NPY) reduces myocardial perfusion and inhibits the force of contraction of the isolated perfused rabbit heart. Regul Pept 6:247–253
Allen JM, Gjörstrup P, Björkman J-A, Ek L, Abrahamsson T, Bloom SR (1986) Studies on cardiac distribution and function of neuropeptide Y. Acta Physiol Scand 126:405–411
Andrews PC, Hawke D, Shively JE, Dixon JE (1985) A nonamidated peptide homologous to porcine peptide YY and neuropeptide YY. Endocrinology 116:2677–2681
Balasubramaniam A, Grupp I, Matlib MA, Benza R, Jackson RL, Fisher JE, Grupp G (1988) Comparison of the effects of neuropeptide Y (NPY) and 4-norleucine-NPY on isolated perfused rat hearts; effects of NPY on atrial and ventricular strips ofrat heart and on rabbit heart mitochondria. Regul Pept 21:289–299
Bjenning C, Holmgren S (1988) Neuropeptides in the fish gut. A study of evolutionary trends. Histochemistry 88:155–163
Burkhardt-Holm P, Holmgren S (1988) A comparison of regulatory neuropeptides in the intestine of two stomachless teleosts (Poecilia reticulata and Leuciscus idus melanotus) under feeding and starving conditions. Cell Tissue Res 255:245–254
Corder R, Lowry PJ, Wilkinson SJ, Ramage AG (1986) Comparison of the haemodynamic actions of neuropeptide Y, angiotensin II and noradrenaline in anaesthetized cats. Eur J Pharmacol 121:25–30
Costa M, Buffa R, Furness JB, Solcia E (1980) Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations. Histochemistry 65:157–169
Falck B, Owman Ch (1965) A detailed methodological description of the fluorescence method for the cellular demonstration of biogenic monoamines. Acta Univ Lundensis 7:1–23
Gannon BJ (1971) A study of the dual innervation of teleost heart by a field stimulation technique. Comp Gen Pharmacol 2:175–183
Gibbins IL, Morris JL, Furness JB, Costa M (1988) Innervation of systemic blood vessels. In: Burnstock G, Griffith S (eds) Non-adrenergic innervation of blood vessels. CRC press, Australia (in press)
Gray TS, Morley JE (1986) Neuropeptide Y: Anatomical distribution and possible function in the mammalian nervous system. Life Sci 38:389–401
Holmgren S (1977) Regulation of the heart of a teleost, Gadus morhua, by autonomic nerves and circulating catecholamines. Acta Physiol Scand 99:62–74
Holmgren S (1978) Sympathetic innervation of the coeliac artery from a teleost, Gadus morhua. Comp Biochem Physiol 60C: 27–32
Holmgren S, Nilsson S (1983) Bombesin-, gastrin/CCK-, 5-hydroxytryptamine-, neurotensin-, somatostatin-, and VIP-like immunoreactivity and catecholamine fluorescence in the gut of the elasmobranch, Squalus acanthias. Cell Tissue Res 234:595–618
Kuramoto H (1987) An immunohistochemical study of chromaffin cells and nerve fibres in the adrenal gland of the bullfrog Rana catesbeiana. Arch Histol Jpn 50:15–38
Lundberg JM, Tatemoto K, Terenius L, Hellström PM, Mutt V, Hökfelt T, Hamberger B (1982) Localization of peptide YY (PYY) in gastrointestinal endocrine cells and effects on intestinal blood flow and motility. Proc Natl Acad Sci USA 79:4471–4475
Lundberg JM, Terenius L, Hökfelt T, Tatemoto K (1984) Comparative immunohistochemical and biochemical analysis of pancreatic polypeptide-like peptides with special reference to presence of neuropeptide Y in central and peripheral neurons. J Neurosci 4:2376–2386
Morris JL, Gibbins IL, Campbell G, Murphy R, Furness JB, Costa M (1986) Innervation of the large arteries and heart of the toad (Bufo marinus) by adrenergic and peptide-containing neurons. Cell Tissue Res 243:171–184
Nilsson S (1983) Autonomic nerve function in the vertebrates. Springer, Berlin Heidelberg New York, pp 1–253
Nilsson S, Holmgren S, Grove DJ (1975) Effects of drugs and nerve stimulation on the spleen and arteries of two species of dogfish, Scyliorhinus canicula and Squalus acanthias. Acta Physiol Scand 95:219–230
Noe BD, McDonald JK, Greiner F, Wood JG, Andrews PC (1986) Anglerfish islets contain NPY immunoreactive nerves and produce the NPY analog aPY. Peptides 7:147–154
Opdyke DF, Holcombe RF (1978) Effect of angiotensins and epinephrine on vascular resistance of isolated dogfish gut. Am P Physiol 234:R196-R200
Rioux F, Bachelard H, Martel JC, St-Pierre S (1986) The vasoconstrictor effect of neuropeptide Y and related peptides in the guinea pig isolated heart. Peptides 7:27–31
Stjärne L, Lundberg JM, Åstrand P (1986) Neuropeptide Y — a cotransmitter with noradrenaline and adenosine 5′-triphosphate in the sympathetic nerves of the mouse vas deferens? A biochemical, physiological and electropharmacological study. Neuroscience 18:151–166
Tatemoto K, Carlquist M, Mutt V (1982) Neuropeptide Y — a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature 296:659–660
Uddman R, Ekblad E, Edvinsson L, Håkansson R, Sundler F (1985) Neuropeptide Y-like immunoreactivity in perivascular nerve fibres of the guinea pig. Regul Pept 10:243–257
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Bjenning, C., Driedzic, W. & Holmgren, S. Neuropeptide Y-like immunoreactivity in the cardiovascular nerve plexus of the elasmobranchs Raja erinacea and Raja radiata . Cell Tissue Res. 255, 481–486 (1989). https://doi.org/10.1007/BF00218782
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DOI: https://doi.org/10.1007/BF00218782