Cell and Tissue Research

, Volume 178, Issue 3, pp 333–339 | Cite as

DNA-synthesizing cells in human fetal thymus

  • C. Röpke
  • B. van Deurs
  • P. E. Høyer


Fragments and suspensions of human fetal thymus were incubated in the presence of 3H-TdR to permit study of the distribution and morphology of DNA-synthesizing cells. Results of light and EM autoradiography showed that 1. although DNA-synthesizing cells were present in the medulla, the vast majority of these cells were localized in the thymic cortex, 2. cells with the typical EM appearance of small lymphocytes and lymphoid blast cells both synthesized DNA, and 3. cells in S-phase were predominantly 8 to 12 μm in size.

Key words

Thymus, Human fetus Lymphocytes DNA synthesis Autoradiography Electron microscopy 


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  1. Borum, K.: Pattern of cell production and cell migration in mouse thymus studied by autoradiography. Scand. J. Haemat. 5, 339–352 (1968)Google Scholar
  2. Cleveland, W.W., Fogel, B.J., Brown, W.T., Kay, H.E.M.: Foetal thymic transplant in a case of diGeorge's syndrome. Lancet 1968 II, 1211–1214Google Scholar
  3. Everett, N.B., Tyler, R.W.: Lymphopoiesis in the thymus and other tissues: Functional implications. Int. Rev. Cytol. 22, 205–237 (1967)Google Scholar
  4. Hinrichsen, K.: Zellteilungen und Zellwanderungen im Thymus der erwachsenen Maus. Z. Zellforsch. 68, 427–444 (1965)Google Scholar
  5. Osmond, D.G.: The origins, lifespans and circulation of lymphocytes. In: Proc. of the Sixth Leucocyte Cult. Conf. Edit. by M.R. Schwarz, pp. 3–32. New York: Academic Press, Inc. 1970Google Scholar
  6. Osmond, D.G., Miller, S.C., Yoshida, Y.: Kinetic and haemopoietic properties of lymphoid cells in the bone marrow. In: Ciba Found. Symp. 13. Edit. by J.F. Loutit, pp. 131–156. Amsterdam: Ass. Scient. Publ. (1973)Google Scholar
  7. Papiernik, M.: Ontogeny of the human lymphoid system: Study of the cytological maturation and the incorporation of tritiated thymidine and uridine in the foetal thymus and lymph node and in the infantile thymus. J. cell. Physiol. 80, 235–242 (1972)Google Scholar
  8. Potmesil, M., Goldfeder, A.: Nucleolar morphology and cell proliferation kinetics of thymic lymphocytes. Exp. Cell Res. 77, 31–40 (1973)Google Scholar
  9. Raff, M.C.: T and B lymphocytes and immune responses. Nature (Lond.) 242, 19–23 (1973)Google Scholar
  10. Röpke, C, Everett, N.B.: Life span of small lymphocytes in the thymolymphatic tissues of normal and thymus-deprived BALB/C mice. Anat. Rec. 183, 83–94 (1975)Google Scholar
  11. Sainte-Marie, G., Leblond, C.P.: Thymus-cell population dynamics. In: The thymus in immunobiology. Edit. by R.A. Good, A.E. Gabrielsen, pp. 207–235. New York: Harper & Row 1964Google Scholar
  12. Stites, D.P., Caldwell, J., Carr, M.C., Fudenberg, H.H.: Ontogeny of immunity in humans. Clin. Imm. & Immunopath. 4, 519–527 (1975)Google Scholar
  13. Wara, D.W., Golbus, M.S., Ammann, A.J.: Fetal thymus glands obtained from prostaglandin-induced abortions. Transplantation 18, 387–390 (1974)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • C. Röpke
    • 1
  • B. van Deurs
    • 1
  • P. E. Høyer
    • 1
  1. 1.Anatomy Department AUniversity of CopenhagenDenmark

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