Summary
An ontogenetic approach was used to obtain information about the relation between structure and function of lymphoid tissues. In particular the development of the capacity to trap immune complexes was studied in relation to the development of the lymphoid compartments. For this purpose isologous horseradish (HRP)-anti-HRP complexes were injected into neonatal rats, and their fate was studied in the spleen and popliteal lymph nodes. Immune-complex trapping occurred as soon as primary follicles could be recognized; without follicles no trapping was observed. Several explanations for this simultaneous development of trapping capacity and follicular structure are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- i.v. :
-
intravenous(ly)
- s.c. :
-
subcutaneous(ly)
- HRP :
-
horseradish peroxidase
- MZ :
-
marginal zone
- PALS :
-
periarteriolar lymphocyte sheath
References
Brächet J (1953) The use of basic dyes and ribonuclease for the cytochemical detection of ribonucleic acid. J Micr Sci 94:1–10
Brown JC, Harris G, Papamichail M, Sljivić VS, Holborow EJ (1973) The localization of aggregated human γ-globulin in the spleens of normal mice. Immunology 24:955–968
Chen LL, Adams JC, Steinman RM (1978) Distribution of horseradish peroxidase (HRP) — anti-HRP immune complexes in mouse spleen with special reference to follicular dendritic cells. J Cell Biol 77:148–164
Davis BJ, Ornstein L (1959) High resolution enzyme localization with a new diazo reagent “Hexazonium Pararosaniline”. J Histochem Cytochem 7:297–298
Eikelenboom P (1978) Dendritic cells in rat spleen follicles. A combined immuno — and enzyme histochemical study. Cell Tissue Res 190:79–87
Eikelenboom P, Levenbach MGE, van den Brink HR, Streefkerk JG (1979) Development of T and B cellareas in peripheral lymphoid organs of the rat. Anat Rec 194:523–538
Groscurth P (1980) Non-lymphatic cells in the lymph node cortex of the mouse. II Postnatal development of the interdigitating cells and the dendritic reticular cells. Pathol Res Pract 169:235–254
Hanna MG, Szakal AK (1968) Localization of 125I-labeled antigen in germinal centers of mouse spleen: histologic and ultrastructural autoradiographic studies of the secondary immune reaction. JImmunol 101(5):949–962
Heusermann U, Zurborn K-H, Schroeder L, Stutte HJ (1980) The origin of the dendritic reticulum cell. An experimental enzyme — histochemical and electron microscopic study on the rat spleen. CellTissue Res 209:279–294
Humphrey JH, Frank MM (1967) The localization of non-microbial antigens in the draining lymphnodes of tolerant, normal and primed rabbits. Immunolgy 13(1):87–100
Kamperdijk EAW, Raaijmakers EM, de Leeuw JHS, Hoefsmit EChM (1978) Lymph node macro-phages and reticulum cells in the immune response. I The primary response to parathyroid vaccine. Cell Tissue Res 192(1):1–23
Klaus GGB (1978) The generation of memory cells. II Generation of B memory cells with preformedantigen-antibody complexes. Immunology 34:643–652
Klaus GGB, Humphrey JH, Kunkl A, Dongworth DW (1980) The follicular dendritic cell: its role inantigen presentation in the generation of immunological memory. Immunol Rev 53:3–28
Lowry OH, Rosebrough NJ, Farr AL, Randail RJ (1951) Protein measurement with the Folin phenolreagent. J Biol Chem 193:265–275
Mandel TE, Phipps RP, Abbot A, Tew JG (1980) The follicular dendritic cell: long term antigen retention during immunity. Immunol Rev 53:29–60
Marshall AHE (1956) Outline of the cytology and pathology of the reticular tissue. Edinburgh: Oliver and Boyd
Nossal GJV, Ada GL (1971) Antigens, lymphoid cells and the immune response. In: Dixon FJ jr, Kunkel HG, Acad Press, New York and London
Nossal GJV, Abbot A, Mitchel J, Lummus Z (1968) Antigens in immunity XV Ultrastructural features of antigen capture in primary and secondary follicles. J Exp Med 127:277–289
Papamichail M, Gutierrez C, Embling P, Holborow EJ, Pepys MB (1975) Complement dependence of localization of aggregated IgG in germinal centres. Scand J Immunol 4:343–347
Pearse AGE (1968) Histochemistry, theoretical and applied VolI, 3rd ed, Edinburgh: Churchill Livingstone
Rooijen N van (1972) Antigens in the spleen. The non-specificity of the follicles in the process of antigen trapping and the role of antibody. Immunology 22:757–765
Rooijen N van (1974) Mechanism of follicular antigen trapping. Evidence for a two cell mechanismusing double isotope autoradiography. Immunology 27:617–622
Rooijen N van (1975) Immune complexes in the spleen. The difference between competitive inhibition ofimmune complex trapping in spleen follicles and inhibition by paratyphoid vaccine. Immunology 28:115–1163
Rooijen N van (1976) Autoradiography and immuno-peroxidase techniques applied to the same tissuesection. J Immunol Methods 10:379–383
Rooijen N van (1978) Binding of labelled antigens and immune complexes to macrophages and dendriticcells in cryostat sections of normal mouse spleen. Acta Morphol Neerl Scand 16:121–127
Rooijen N van (1980) Immune complex trapping in lymphoid follicles: a discussion on possiblefunctional implications. In: Manning, MJ (ed) Phylogeny of immunological memory. Prog Int SympImmunological memory, Elsevier/North-Holland, Amsterdam New York Oxford
Veerman AJP (1975) The postnatal development of the white pulp in the rat spleen and the onset of immunocompetence against a thymus independent and a thymus dependent antigen. Z Immun Forsch 150:45–59
Veerman AJP, van Ewijk (1975) White pulp compartments in the spleen of rats and mice. A light and electron microscopic study of lymphoid and non-lymphoid celltypes in T and B areas. Cell Tissue Res 156:417–441
Veerman AJP, Rooijen N van (1975) Lymphocyte capping and lymphocyte migration as associatedevents in the in vivo antigen trapping process. An electron microscopic autoradiographic study in the spleen of mice. Cell Tissue Res 161:211–217
Williams AM, Nossal GJV (1966) Ontogeny of the immune response. I. The development of the follicular antigen trapping mechanism. J Exp Med 124:47–56
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dijkstra, C.D., van Tilburg, N.J. & Döpp, E.A. Ontogenetic aspects of immune-complex trapping in the spleen and popliteal lymph nodes of the rat. Cell Tissue Res. 223, 545–552 (1982). https://doi.org/10.1007/BF00218475
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218475