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Synaptic connections of substance P-immunoreactive nerve terminals in the substantia nigra of the rat

A correlated light- and electron-microscopic study

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Summary

A monoclonal antibody that recognises the C-terminal part of substance P was used to study immunoreactive structures in the substantia nigra by the unlabeled antibody, peroxidase-antiperoxidase procedure. Immunoreactivity was present in nerve fibres in all parts of the substantia nigra, particularly in the pars reticulata and pars lateralis. Electron microscopically two types of bouton immunoreactive for substance P were found: Type 1 contained large electron-lucent vesicles, occasional large granulated vesicles and formed symmetrical synapses with dendrites. Type 2 boutons contained smaller, round electron-lucent vesicles, many large granular vesicles and formed asymmetrical synapses (having prominent postjunctional dense bodies) with dendrites and perikarya.

Immunoreactive fibres with varicosities that had been identified light microscopically were studied in serial sections in the electron microscope. Each identified varicosity contained synaptic vesicles and formed a single synapse. An individual fibre formed boutons of only one kind (type 1 or type 2) and could form multiple synapses with the same neuron. Thus, an identified fibre in the pars compacta had eight varicosities, each of which was in synaptic contacts (type 2) with the dendrites or soma of the same neuron.

The results are consistent with the concept that substance P is a synaptic transmitter in the substantia nigra and indicate that neurons in this region may receive a significant input from substance P-containing afferents, and that there are at least two types of such afferent fibres.

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Authors and Affiliations

  1. First Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary

    Peter Somogyi

  2. Department of Human Anatomy, Oxford University, Oxford, UK

    J. V. Priestley & A. C. Cuello

  3. Department of Pharmacology, Oxford University, Oxford, UK

    J. V. Priestley, A. C. Cuello, A. D. Smith & J. P. Bolam

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  1. Peter Somogyi
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  2. J. V. Priestley
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  3. A. C. Cuello
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  4. A. D. Smith
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  5. J. P. Bolam
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Somogyi, P., Priestley, J.V., Cuello, A.C. et al. Synaptic connections of substance P-immunoreactive nerve terminals in the substantia nigra of the rat. Cell Tissue Res. 223, 469–486 (1982). https://doi.org/10.1007/BF00218469

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