Summary
Nuclei isolated from D. discoideum and incubated in vitro with 3H-NAD synthesise poly(ADP-Rib). The optimum incubation conditions for the poly(ADP-Rib) polymerase were determined. The Km of the enzyme is 18 μm NAD and it is inhibited by nicotinamide. Most of the poly(ADP-Rib) synthesised is attached to nuclear proteins.
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Rickwood, D., Osman, M.S. Characterisation of poly(ADP-Rib) polymerase activity in nuclei from the slime mould dictyostelium discoideum. Mol Cell Biochem 27, 79–84 (1979). https://doi.org/10.1007/BF00218351
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DOI: https://doi.org/10.1007/BF00218351