Summary
To examine the morphological evidence of the nature of hormone release from castration cells the author gonadectomized neonatal male and female rats. Twenty-eight days after the operation the animals were pretreated with estrogen, progesterone, or estrogen and progesterone in combination, every other day for one week, and then injected with LHRH 30 min before sacrifice. The administration of LHRH elevated remarkably the serum levels of gonadotrophins (LH and FSH) of neonatally gonadectomized rats. The steroid-pretreated animals showed higher serum levels of gonadotrophins than controls. Simultaneously, active exocytosis of secretory granules was observed in the hypertrophic gonadotrophs or castration cells of neonatally gonadectomized and steroid-treated rats following the injection of LHRH. These results indicate that hypertrophic gonadotrophs or castration cells also release hormone(s) by exocytosis of secretory granules.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arimura A, Debuljuk L, Shiino M, Rennets EG, Schally AV (1973) Follicular stimulation by chronic treatment with synthetic LH-releasing hormone in hypophysectomized female rats bearing pituitary grafts. Endocrinology 92:1507–1514
Debeljuk L, Arimura A, Shiino M, Rennels EG, Schally AV (1973) Effects of chronic treatment with LH/FSH-RH in hypophysectomized pituitary-grafted male rats. Endocrinology 92:921–930
Goldman BD, Grazia YR, Kamberi IA, Porter JC (1971) Serum gonadotropin concentrations in intact and castrated neonatal rats. Endocrinology 88:771–776
Kurosumi K (1968) Functional classification of cell types of the anterior pituitary gland accomplished by electron microscopy. Arch Histol Jpn 29:329–362
Kurosumi K, Inoue K (1980) Surface pits of typical gonadotrophs and castration. Cells of the rat anterior pituitary suggestive of exocytosis and micropinocytosis. Arch Histol Jpn 43:373–382
Kurosumi K, Oota Y (1968) Electron microscopy of two types of gonadotrophs in the anterior pituitary glands of persistent estrous and diestrous rats. Z Zellforsch 85:34–46
McCann SM, Porter SC (1969) Hypothalamic pituitary stimulating and inhibiting hormones. Physiol Rev 49:240–284
Midgley Jr AR (1967) Radioimmunoassay for human follicle stimulating hormone. J Clin Endocrinol Metab 27:295–299
Niswender GD, Midgley Jr AR, Monroe SE, Reichert Jr LE (1968) Radioimmunoassay for rat luteinizing hormone with antiovine LH serum and ovine LH-131I. Proc Soc Exp Biol 128:807–811
Pelletier G, Peillon F, Vila-Procile E (1971) An ultrastructural study of sites of granule extrusion in the anterior pituitary of the rat. Z Zellforsch 115:501–507
Rennels EG, Bogdanove EM, Arimura A, Saito M, Schally AV (1971) Ultrastructural observations of rat pituitary gonadotrophs following injection of purified porcine LH-RH. Endocrinology 88:1318–1326
Shiino M, Rennels EG (1974) Ultrastructural observations on pituitary gonadotrophs following gonadectomy or administration of LH/FSH-releasing hormone in neonatal rats. Tex Rep Biol Med 32:562–567
Shiino M, Arimura A, Schally AV, Rennels EG (1971) Ultrastructural observations of granule extrusion from rat anterior pituitary cells after injection of LH-releasing hormone. Z Zellforsch 128:152–161
Author information
Authors and Affiliations
Additional information
Supported by NIH Grant HD 11826 and PRO 7187
The author wishes to express his thanks to John Rhode of RIA Core of the Center for Research in Reproductive Biology for his technical help of radioimmunoassay and Shelley Bohne in our laboratory for her excellent assistance of EM study. The author thanks Dr. Edward G. Rennels for his help in preparing the manuscript
Rights and permissions
About this article
Cite this article
Shiino, M. Ultrastructural evidence of gonadotrophin release from castration cells following injection of LHRH in the rat. Cell Tissue Res. 222, 213–222 (1982). https://doi.org/10.1007/BF00218301
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218301