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Distribution of anti-Leu-7, anti-Leu-11a and anti-Leu-M1 immunoreactivity in the brain of the adult rat

Cell and Tissue Research volume 248, pages 305–313 (1987)Cite this article

Summary

This study reports a specific cross-reactivity of the three anti-human-hematopoetic-cell monoclonal antibodies, anti-Leu-7 (HNK-1), anti-Leu-11a (NKP-15), and anti-Leu-M1 (MMA), with different epitopes in the brain of the adult rat. The distribution of these epitopes in rat brain is determined by means of immunohistochemistry in paraffin-embedded frontal serial sections.

The reaction pattern of anti-Leu-11a monoclonal antibody is very similar to that of polyclonal antibodies against the myelin basic protein. Both antisera give a specific reaction with myelinated fibers. Immunoreaction products with the anti-Leu-7 monoclonal antibody are found as diffuse, mostly punctiform material in the neuropil and even more evident as small granules coating the cell surface of many neurons. In the white matter anti-Leu-7 reveals a moderate reactivity, which occurs predominantly as spots and fine-stranded material within the myelinated fiber tracts.

Anti-Leu-M1 immunoreactivity is present between myelinated fiber bundles of the white matter, where it has a reticulate appearance, and as fine-granulated material within the grey matter of the cortex and the nuclei. The characteristic feature in the grey matter is that of irregularly shaped immunopositive plaques, which are often located around small blood vessels. The cytoplasm of glial and neuronal cells appeared negative with this MAB.

The exact topographical distribution of the Leu-7 and Leu-M1 epitopes throughout the rat brain is described. The present hypotheses concerning the nature of this shared antigenicity between hematopoetic cells and nervous tissue are discussed.

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Affiliations

  1. Department of Neuropathology, University of Düsseldorf, Düsseldorf, Federal Republic of Germany

    Guido Reifenberger & Wolfgang Wechsler

  2. C. u. O. Vogt-Institute for Brain Research and Anatomy I, University of Düsseldorf, Düsseldorf, Federal Republic of Germany

    Jürgen K. Mai

  3. Department of Neuropathology, Polish Academy of Sciences, Medical Research Centre, Warszawa, Poland

    Stanislaw Krajewski

Authors
  1. Guido Reifenberger
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  2. Jürgen K. Mai
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  3. Stanislaw Krajewski
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  4. Wolfgang Wechsler
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Supported by the Deutsche Forschungsgemeinschaft, SFB 200

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Reifenberger, G., Mai, J.K., Krajewski, S. et al. Distribution of anti-Leu-7, anti-Leu-11a and anti-Leu-M1 immunoreactivity in the brain of the adult rat. Cell Tissue Res. 248, 305–313 (1987). https://doi.org/10.1007/BF00218197

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  • DOI: https://doi.org/10.1007/BF00218197

Key words

  • Brain
  • Immunohistochemistry
  • Natural killer cells
  • Monocytes
  • Granulocytes
  • Rat