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Polymorphism in the rotifer Asplanchna sieboldi

Fine structure of saccate, cruciform and campanulate females

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Summary

The morphology of saccate, cruciform, and campanulate females of Asplanchna sieboldi was analyzed by light and electron microscopy. No major qualitative variations were observed in the ultrastructure of the hypodermis, gastric gland and yolk gland of females from the different morphotypes. Campanulate females did, however, possess larger secretory granules in their gastric gland as well as larger and more numerous nuclei in their gastric and yolk glands than both saccate and cruciform females.

The structure of the secretory elements in the gastric gland varied between the two clones investigated.

Zusammenfassung

Sackförmige, kreuzförmige und Riesenweibchen von Asplanchna sieboldi wurden mit cytologischen und elektronenmikroskopischen Methoden untersucht. In der Ultrastruktur der Hypodermis, der Magendrüse und des Vitellariums sind die drei Arten ähnlich. Riesenweibchen besitzen im Gegensatz zu den anderen Weibchensorten in der Magendrüse größere Sekretvakuolen. Sie haben in der Magendrüse und im Vitellarium eine größere Anzahl von Zellkernen und eine größere Kernmasse.

Die Feinstruktur der Sekretgranula variiert zwischen den zwei untersuchten Klonen.

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References

  • Akeson, R., Herschman, H.R.: Clonal variations in murine neuroblastoma. Morphologic and antigenic differentiation. Expt. Cell Res. 93, 492–495 (1975)

    Google Scholar 

  • Amano, T., Richelson, E., Nirenbert, M.: Neurotransmitter synthesis by neuroblastoma clones. Proc. nat. Acad. Sci. (Wash.) 69, 258–263 (1972)

    Google Scholar 

  • Beauchamp, P.M.: Recherches sur les rotifères: Les formations tégumentaires et l'appareil digestif. Arch. Zool. Exp. Gén. 10, 1–410 (1909)

    Google Scholar 

  • Bentfeld, M.E.: Studies of oogenesis in the rotifer, Asplanchna. I. Fine structure of the female reproductive system. Z. Zellforsch. 115, 165–183 (1971a)

    Google Scholar 

  • Bentfeld, M.E.: Studies of oogenesis in the rotifer. Asplanchna. II. Oocyte growth and development. Z. Zellforsch. 115, 184–195 (1971b)

    Google Scholar 

  • Birky, C.W., Jr.: The developmental genetics of polymorphism in the rotifer Asplanchna. I. Dietary vitamin E control of mitosis and morphogenesis in embryos. J. exp. Zool. 169, 205–210 (1968)

    Google Scholar 

  • Brodie, A.E.: Development of the cuticle in the rotifer A. brightwelli. Z. Zellforsch. 105, 515–525 (1970)

    Google Scholar 

  • Gilbert, J.J.: Dietary control of sexuality in the rotifer Asplanchna brightwelli Gosse. Physiol. Zool. 41, 14–43 (1968)

    Google Scholar 

  • Gilbert, J.J.: The adaptive significance of polymorphism in the rotifer Asplanchna. Humps in males and females. Oecologia (Berl.) 13, 135–146 (1973a)

    Google Scholar 

  • Gilbert, J.J.: Induction and ecological significance of gigantism in the rotifer Asplanchna sieboldi. Science 181, 63–66 (1973b)

    Google Scholar 

  • Gilbert, J.J.: Effect of tocopherol on the growth and development of rotifers. Amer. J. clin. Nutr. 27, 1005–1016 (1974a)

    Google Scholar 

  • Gilbert, J.J.: Dormancy in rotifers. Trans. Amer. micr. Soc. 93, 490–513 (1974b)

    Google Scholar 

  • Gilbert, J.J.: Polymorphism and sexuality in the rotifer Asplanchna, with special reference to the effects of prey-type and clonal variation. Arch. Hydrobiol. 75, 442–483 (1975)

    Google Scholar 

  • Gilbert, J.J.: Polymorphism in the rotifer Asplanchna sieboldi: Biomass, growth and reproduction rate of the saccate and campanulate morphotypes. Ecology (in press)

  • Gilbert, J.J., Thompson, G.A.: Alpha-tocopherol control of sexuality and polymorphism in the rotifer Asplanchna. Science 159, 734–736 (1968)

    Google Scholar 

  • Glick, M.C., Kimhi, Y., Littauer, U.Z.: Glycopeptides from surface membranes of neuroblastoma cells. Proc. nat. Acad. Sci. (Wash.) 70, 1682–1687 (1973)

    Google Scholar 

  • Goel, S.C., Jurand, A.J.: EM studies on developing cartilage. II. Demonstration and distribution of glycogen in chick and mouse epiphyseal cartilage. J. Embryol. exp. Morph. 28, 153–162 (1972)

    Google Scholar 

  • Herold, R.C., Meadow, N.D.: Age related changes in ultrastructure and histochemistry of rotiferan organs. J. Ultrastruct. Res. 33, 203–218 (1970)

    Google Scholar 

  • Herman, L., Sato, T., Fitzgerald, P.J.: The pancreas. In: Electron microscopic anatomy (S.M. Kurtz, ed.), pp. 59–74. New York-London: Academic Press 1964

    Google Scholar 

  • Hewlett-Packard: HP-65 Stat. Pac. 1, pp. 1–123. Cupertino, California: Hewlett Packard 1974

    Google Scholar 

  • Koehler, J.K.: A fine-structure study of the rotifer integument. J. Ultrastruct. Res. 12, 113–134 (1965)

    CAS  PubMed  Google Scholar 

  • Luft, J.H.: Improvements in epoxy embedding methods. J. biophys. biochem. Cytol. 9, 409–427 (1961)

    Google Scholar 

  • Molenaar, I., Vos, J., Hommes, F.A.: Effect of vitamin E deficiency on cellular membranes. In: Vitamins and hormones, Vol. 30 (Harris, R.S., Diczfalusy, E., Munson, P.L., Glover, J., eds.), pp. 45–82. New York-London: Academic Press 1972

    Google Scholar 

  • Peacock, J., Minna, J., Nelson, P., Nirenberg, M.W.: Use of aminopterin in selecting electrically active neuroblastoma cells. Expt. Cell Res. 73, 367–377 (1972)

    Google Scholar 

  • Powers, J.H.: A case of polymorphism in Asplanchna simulating a mutation. Amer. Natural. (N.Y.) 46, 441–462 and 526–552 (1912)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Rohlf, F.J., Sokal, R.R.: Statistical tables, pp. 253. San Francisco: Freeman and Co. 1969

    Google Scholar 

  • Sakiyama, H., Gross, S.K., Robbins, P.W.: Glycolipid synthesis in normal and virus-transformed hamster cell lines. Proc. nat. Acad. Sci. (Wash.) 69, 872–876 (1972)

    Google Scholar 

  • Siekevitz, P., Palade, G.E.: A cytochemical study on the pancreas of the guinea pig. II. Functional variations in the enzymatic activity of microsomes. J. biophys. biochem. Cytol. 4, 309–318 (1958)

    Google Scholar 

  • Sokal, R.R., Rohlf, F.J.: Biometry (Emerson, R., Kennedy, D., Park, R.B. eds.), pp. 1–776. San Francisco: W.H. Freeman and Co. 1969

    Google Scholar 

  • Spurlock, B.O., Skinner, M.S., Kattine, A.A.: A simple rapid method for staining epoxy-embedded specimens for light microscopy with a polychromatic stain Paragon-1301. Amer. J. clin. Path. 46, 252–258 (1966)

    Google Scholar 

  • Spurr, A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969)

    Google Scholar 

  • Spurr, A.R., Harris, W.M.: Ultrastructure of chloroplasts and chromoplasts in Capsicuum annuum. I. Thylakoid membrane changes during fruit ripening. Amer. J. Bot. 55, 1210–1224 (1968)

    Google Scholar 

  • Storch, V., Welsch, U.: Über den Aufbau des Rotatorienintegumentes. Z. Zellforsch. 95, 405–414 (1969)

    Google Scholar 

  • Vye, M.V., Fishman, D.A.: The morphological alteration of particulate glycogen by en bloc staining with uranyl acetate. J. Ultrastruct. Res. 33, 278–291 (1970)

    Google Scholar 

  • Waniczek, H.: Untersuchungen über einige Arten der Gattung Asplanchna Gosse (A. girodi de Guerne, A. brightwelli Gosse, A. priodonta Gosse). Ann. Mus. Zool. Pol. 8, 109–322 (1930)

    Google Scholar 

  • Yogeeswaran, G., Murray, R.K.: Glycosphingolipids of clonal lines of mouse neuroblastoma and neuroblastoma XL hybrids. J. biol. Chem. 248, 1231–1239 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Dartmouth College, Hanover, New Hampshire, USA

    Elizabeth Samodai Wurdak & John J. Gilbert

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  1. Elizabeth Samodai Wurdak
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  2. John J. Gilbert
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Additional information

Supported by Public Health Service research grant 1 R01 HD 08210 from the National Institute of Child Health and Human Development, and by Public Health Service research career development award K04-GM-7055 from the National Institute of General Medical Sciences granted to John J. Gilbert

We thank M.E. Kabay for advice on statistics and both him and T.M. Frost for their valuable criticism of the manuscript

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Wurdak, E.S., Gilbert, J.J. Polymorphism in the rotifer Asplanchna sieboldi . Cell Tissue Res. 169, 435–448 (1976). https://doi.org/10.1007/BF00218145

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