Summary
In untreated adult male albino rats changes in nuclear volume within the periportal and the perivenous zones of the liver acinus were measured during a full day and night cycle. Only the perivenous zone displayed circadian rhythmic changes as expressed in the sinus-like course of the nuclear volume-distribution graph (polynomial) with a maximum between 16.00 h and 18.00 h and a minimum at 8.00 h. In all groups studied zonal heterogeneity of the means of nuclear volume and the percent-distribution graphs of nuclear volume classes were retained during the full cycle. Within each zone, however, distribution curves of nuclear volume classes were homogeneous indicating an equally directed trend in time-dependent changes in nuclear volume.
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References
Armitage P (1971) Statistical methods in medical research. Blackwell, Oxford London Edinburgh Boston Melbourne
Bucher O, Suppan P (1967) Amitose et fusion nucléaire au cours du rhythme circadien. In: v Mayersbach H (ed) The cellular aspects of biorhythms. Springer, Berlin Heidelberg New York, pp 124–132
Caspersson T, Holmgren Hj (1934) Variationen der Kerngröße während der verschiedenen Phasen der Leberarbeit. Anat Anz 79:53–59
Draper NR, Smith H (1966) Applied regression analysis. Wiley, New York London Sydney
Eger W (1952) Das zentrale und periphere Funktionsfeld des Leberläppchens unter der Einwirkung von Äthyl und Methylalkohol. Med Monatsschr 6:363–367
Eichner D (1963) Zur Rationalisierung der Vermessung und Registrierung von Zellkernen. Z mikrosk anat Forsch 69:381–387
Geissner S, Greenhouse SW (1958) An extension of Box's results on the use of the F distribution in multivariate analysis. Ann Math Stat 29:885–891
Halberg F (1959) Physiologic 24-h periodicity; general and procedural considerations with reference to the adrenal cycle. Z Vitamin Ferment Hormon Forsch 10:225–296
Hildebrand R (1977) Karyometrische Untersuchungen an unbehandelten Ratten. Verh Anat Ges 71:223–228
Hildebrand R (1980) Nuclear volume and cellular metabolism. Adv Anat Embryol Cell Biol 60. Springer, Berlin Heidelberg New York
Hildebrand R, Wegener E (1980) Beitrag zur rationellen Vermessung von Zellkernvolumina. Gegenbaurs Morph Jahrb 126:965–968
Inke G, Palkovits M, Gyarfas J (1958) Über methodische Fragen der Kernvariationsstatistik. VI. Bewertung der Gruppierungsmethoden. Acta Morphol Acad Sci Hung 8:263–271
Jacobj W (1942) Die verschiedenen Arten des gesetzmäßigen Zellwachstums und ihre Beziehung zu Zellfunktion, Umwelt, Krankheit, maligner Geschwulstbildung und innerem Bauplan. W Roux Arch Entw Mech 141:584–692
Jerusalem CH, Eling W, Jap P (1970) Histochemische und elektronenmikroskopische Veränderungen der Leberzelle im Tagesrhythmus und unter experimentellen Bedingungen. Acta Histochem 36:168–206
Jungermann K, Sasse D (1978) Hetrogeneity of liver parenchyma cells. TIBS 3:198–202
Le Bouton AV (1974) Growth, mitosis and morphogenesis of the simple liver acinus in neonatal rats. Dev Biol 41:22–30
Mayersbach H v (1981) Chronobiological aspects in histochemistry. In: Graumann W, Neumann K (eds) Handb d Histochemie Vol I, Allgemeine Methodik, Part 4. Fischer, Stuttgart New York, pp 1–152
Palkovits M, Fischer J (1968) Karyometric investigations. Akadémiai Kiadó, Budapest
Pearse AGE (1968) Histochemistry, theoretical and applied. Vol 1 Churchill, London
Petry G, Hildebrand R (1981) Plastische Rekonstruktion praeterminaler und terminaler Gefäße in der Leber von Mensch und Ratte. Verh Anat Ges 75:719–720
Rappaport AM (1963) Acinar units and the pathophysiology of the liver. In: Rouiller Ch (ed) The liver, morphology, biochemistry, physiology. Vol 1. Academic Press, New York London, pp 265–328
Rappaport AM (1975a) The microcirculatory hepatic unit. In: Gerok W, Sickinger K (eds) Drugs and the liver. Arzneimittel und Leber. Schattauer, Stuttgart New York, pp 425–434
Rappaport AM (1975b) Anatomic considerations. In: Schiff L (ed) Diseases of the liver. Lippincott, Philadelphia, pp 1–50
Rappaport AM, Borowy ZJ, Lougheed WM, Lotto WN (1954) Subdivision of hexagonal liver lobules into a structural and functional unit. Anat Rec 119:11–33
Suppan P (1966) Le cycle diurne hépatique. Acta Anat 65:584–593
Teutsch HF (1981) Chemomorphology of liver parenchyma. Prog Histochem Cytochem 14, No 3. Fischer, Stuttgart New York
Vogt R (1976) Zum Problem der Klasseneinteilung bei der Karyometrie. Inaug Diss Med Fak, Münster
Waerden BL v d (1971) Mathematische Statistik. Springer, Berlin Heidelberg New York
Winer BJ (1972/72) Statistical principles in experimental design. Mc Graw Hill, Tokyo
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Dedicated to Prof. Dietrich Eichner on the occasion of his 60th birthday
Supported by the Deutsche Forschungsgemeinschaft (Hi 318/2-1)
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Schultz, M., Hildebrand, R. Karyometric investigation on circadian rhythmic changes in the periportal and perivenous zones of the acinus of the rat liver. Cell Tissue Res. 231, 643–654 (1983). https://doi.org/10.1007/BF00218122
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DOI: https://doi.org/10.1007/BF00218122