Summary
Effects of a short-term exposure to continuous darkness on 24-h morphological variations in pinealocytes in the superficial pineal of the Chinese hamster (Cricetulus griseus) were examined. Pinealocytes contained type-1, -2 and -3 synaptic ribbons (SR), which had a central dense structure showing rod-like, various and ring-like profiles, respectively, and the quantity of each type of SR was expressed by SR index. 24-h changes in the type-1 and type-3 SR indices persisted in darkness and thus may be endogenous in nature. As under alternating light and dark (LD) conditions, the type-2 SR indices were almost constant over a 24-h period under continuous darkness, but the indices were larger in animals under darkness than in those under LD conditions. The 24-h variations in the nuclear and cytoplasmic volumes were abolished after exposing animals to darkness for 7 days, suggesting that these rhythms may be regulated exogenously. The amount of condensed chromatin exhibited a circadian change; this rhythm persisted under darkness. The results suggest that 24-h variations in the nuclear and cytoplasmic volumes in pinealocytes of the Chinese hamster are regulated by mechanisms different from those controlling the rhythms in SR and chromatin, and that the changes in the nuclear and cytoplasmic volumes and chromatin are related to the change in synthetic activity of pinealocytes.
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References
Abe K, Matsushima YS, Mita M (1983) Estimation of the mean size of spheres from the profiles in ultrathin sections: a stereological method. J Electron Microsc 32:57–60
Axelrod J (1974) The pineal gland: a neurochemical transducer. Science 184:1341–1348
Becker UG, Vollrath L (1983) 24-hour-variation of pineal gland volume, pinealocyte nuclear volume and mitotic activity in male Sprague-Dawley rats. J Neural Transm 56:211–221
Brownstein M, Axelrod J (1974) Pineal gland: 24-hour rhythm in norepinephrine turnover. Science 184:163–165
Derenzini M, Bonetti E (1975) Cycloheximide-induced ultrastructural changes in hepatocyte nuclei in partially hepatectomized rats. Virchows Arch B Cell Pathol 19:115–125
Derenzini M, Marinozzi V, Novello F (1976) Effects of α-amanitine on chromatin in regenerating rat hepatocytes. A biochemical and morphologic study. Virchows Arch B Cell Path 20:307–318
Derenzini M, Pession-Brizzi A, Bonetti E, Novello F (1979) Relationship between ultrastructure and function of hepatocyte chromatin: a study with adrenalectomized rats after cortisol administration. J Ultrastruct Res 67:161–179
Diehl BJM (1981) Time-related changes in size of nuclei of pinealocytes in rats. Cell Tissue Res 218:427–438
Diehl BJM, Heidbüchel U, Welker HA, Vollrath L (1984) Day/night changes of pineal gland volume and pinealocyte nuclear size assessed over 10 consecutive days. J Neural Transm 60:19–29
Fakan S, Puvion E (1980) The ultrastructural visualization of nucleolar and extranucleolar RNA synthesis and disbribution. Int Rev Cytol 65:255–299
Frenster JH (1974) Ultrastructure and function of heterochromatin and euchromatin. In: Busch H (ed) The cell nucleus. Vol I, Academic Press, New York London, pp 565–580
Hewing M (1980) Synaptic ribbons in the pineal system of normal and light deprived golden hamsters. Anat Embryol 159:71–80
Kachi T, Ito T (1977) Neural control of glycogen content and its diurnal rhythm in mouse pineal cell. Am J Physiol 232:E584-E589
Kachi T, Matsushima S, Ito T (1974) Effect of continuous darkness on diurnal rhythm in glycogen content in pineal cells of the mouse: a semi-quantitative histochemical study. Anat Rec 179:405–410
King TS, Dougherty WJ (1982a) Age-related changes in pineal “synaptic” ribbon populations in rats exposed to continuous light or darkness. Am J Anat 163:169–179
King TS, Dougherty WJ (1982b) Effect of denervation on “synaptic” ribbon populations in the rat pineal gland. J Neurocytol 11:19–28
Krzyzowska-Gruca S, Zborek A, Gruca S (1983) Distribution of interchromatin granules in nuclear matrices obtained from nuclei exhibiting different degree of chromatin condensation. Cell Tissue Res 231:427–437
Kurumado K, Mori W (1980) Pineal synaptic ribbons in blinded rats. Cell Tissue Res 208:229–235
Lew GM, Payer A, Quay WB (1982) The pinealocyte nucleolus. Ultrastructural and stereological analysis of twenty-four-hour changes. Cell Tissue Res 224:195–206
Lew GM, Washko K, Quay WB (1984) Quantitation of ultrastructural twenty-four-hour changes in pineal nuclear dimensions. J Pineal Res 1:61–68
Lues G (1971) Die Feinstruktur der Zirbeldrüse normaler, trächtiger und experimentell beeinflußter Meerschweinchen. Z Zellforsch 114:38–60
Martinez Soriano F, Welker HA, Vollrath L (1984) Correlation of the number of pineal “synaptic” ribbons and spherules with the level of serum melatonin over a 24-hour period in male rabbits. Cell Tissue Res 236:555–560
Matsushima S, Morisawa Y, Aida I, Abe K (1983a) Circadian variations in pinealocytes of the Chinese hamster, Cricetulus griseus. A quantitative electron-microscopic study. Cell Tissue Res 228:231–244
Matsushima S, Sakai Y, Aida I (1983b) Effects of melatonin on synaptic ribbons in pinealocytes of the Chinese hamster, Cricetulus griseus. A quantitative electron-microscopic study. Cell Tissue Res 233:59–67
McNulty JA (1981) Synaptic ribbons in the pineal organ of the goldfish: circadian rhythmicity and the effects of constant light and constant darkness. Cell Tissue Res 215:491–497
Milner GR, Hayhoe FGJ (1968) Ultrastructural localization of nucleic acid synthesis in human blood cells. Nature 218:785–787
Morisawa Y, Matsushima S (1979) Effects of continuous lighting or continuous darkness on large granulated vesicles in sympathetic nerve fibers of the mouse pineal — quantitative electron microscopic observations. J Neural Transm 46:291–301
Mukai S, Matsushima S (1980) Effect of continuous darkness on diurnal rhythms in small vesicles in sympathetic nerve endings of the mouse pineal — quantitative electron microscopic observations. J Neural Transm 47:131–143
Nir I, Hirschmann N, Sulman FG (1971) Diurnal rhythms of pineal nucleic acids and protein. Neuroendocrinology 7:271–277
Quay WB (1974) Pineal chemistry in cellular and physiological mechanisms. Charles C Thomas, Springfield, pp 233–241
Quay WB, Renzoni A (1966) Twenty-four-hour rhythms in pineal mitotic activity and nuclear and nucleolar dimensions. Growth 30:315–324
Romijn HJ (1975) The ultrastructure of the rabbit pineal gland after sympathectomy, parasympathectomy, continuous illumination, and continuous darkness. J Neural Transm 36:183–194
Snedecor GW, Cochran WG (1980) Statistical methods. The Iowa State University Press, Ames
Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Tokuyasu K, Madden SC, Zeldis LJ (1968) Fine structural alterations of interphase nuclei of lymphocytes stimulated to growth activity in vitro. J Cell Biol 39:630–660
Vázquez-Nin GH, Echeverria OM, Molina E, Fragoso J (1978) Effects of ovariectomy and estradiol injection on nuclear structures of endometrial epithelial cells. Acta Anat 102:308–318
Vic P, Garcia M, Humeau C, Rochefort H (1980) Early effect of estrogen on chromatin ultrastructure in endometrial nuclei. Mol Cell Endocrinol 19:79–92
Vollrath L (1973) Synaptic ribbons of a mammalian pineal gland. Circadian changes. Z Zellforsch 145:171–183
Vollrath L, Howe C (1976) Light and drug induced changes of epiphysial synaptic ribbons. Cell Tissue Res 165:383–390
Vollrath L, Huss H (1973) The synaptic ribbons of the guinea-pig pineal gland under normal and experimental conditions. Z Zellforsch 139:417–429
Vollrath L, Schultz RL, McMillan PJ (1983) “Synaptic” ribbons and spherules of the guinea pig pineal gland: inverse day/night differences in number. Am J Anat 168:67–74
Welsh MG, Cameron IL, Reiter RJ (1979) The pineal gland of the gerbil, Meriones unguiculatus. II. Morphometric analysis over a 24-hour period. Cell Tissue Res 204:95–109
Wurtman RJ, Axelrod J, Sedvall G, Moore RY (1967) Photic and neural control of the 24-hour norepinephrine rhythm in the rat pineal gland. J Pharmacol Exp Ther 157:487–492
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Sakai, Y., Aida, I. & Matsushima, S. Effect of continuous darkness on circadian morphological rhythms in pinealocytes of the Chinese hamster, Cricetulus griseus . Cell Tissue Res. 245, 127–134 (1986). https://doi.org/10.1007/BF00218093
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DOI: https://doi.org/10.1007/BF00218093