Summary
Gap junctions exist in the septa between the segments of the lateral giant axons in the ventral nerve cord of the crayfish Procambarus. A large increase in the resistance (uncoupling) of these gap junctions was brought about by mechanical injury to the axonal segments. Both thin sections and freeze-fracture preparations were used to monitor the morphological changes which occurred up to 45 min after injury.
There was no apparent change in the organization (a loose polygonal array) of the intramembrane particles which make up the junctional complex up to 45 min after injury. In some instances, however, the intramembrane particles appeared to have moved away from the junctional area. Other junctional regions were internalized and appeared similar to what have been called annular gap junctions. Also at this time (20–25 min after injury), a dense cytoplasmic plug formed in uninjured axon near the junctional region. It is concluded that the gap junctions that exhibit a loose polygonal organization of the intramembrane particles may be either in a state of low resistance (coupled) or a state of high resistance (uncoupled).
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References
Albertini DF, Anderson E (1974) The appearance and structure of intercellular connections during the ontogeny of the rabbit ovarian follicle with particular reference to gap junctions. J Cell Biol 63:234–250
Asada Y, Bennett MVL (1971) Experimental alteration of coupling resistance at an electrotonic synapse. J Cell Biol 49:159–172
Baldwin K (1979) Cardiac gap junction configuration after an uncoupling treatment as a function of time. J Cell Biol 82:66–75
Campbell KL, Albertini DF (1981) Freeze-fracture analysis of gap junction disruption in rat ovarian granulosa cells. Tissue Cell 13(4):651–668
Dahl G, Isenberg G (1980) Decoupling of heart muscle cells: correlation with increased cytoplasmic calcium activity and with changes of nexus ultrastructure. J Membr Biol 53:63–75
Hanna RB (1979) An improved method for cleaning and handling freeze-fracture replicas. (ed) CW Bailey, 37th Ann Proc Electron Microscopy Soc of America: pp 364–365
Hanna RB, Keeter JS, Pappas GD (1978a) The fine structure of a rectifying electrotonic synapse. J Cell Biol 79:764–773
Hanna RB, Spray DC, Model PG, Harris AL, Bennett MVL (1978b) Ultrastructure and physiology of gap junctions of an amphibian embryo, effects of CO2. Biol Bull 155:442
Hanna RB, Pappas GD, Bennett MVL (1979) Structural changes associated with increased coupling resistance in the septate axon of the crayfish. Biol Bull 157(2):370
Hanna RB, Reese TS, Ornberg RL, Spray DC, Bennett MVL (1981) Fresh frozen gap junctions: Resolution of structural detail in the coupled and uncoupled states. J Cell Biol 91:125a
Kistler J, Bullivant S (1980) The connexon order in isolated lens gap junction. J Ultrastruct Res 72:27–381
Lane NJ, Swales LS (1978) Changes in the blood-brain barrier of the central nervous system in the blowfly during development, with special reference to the formation and disaggregation of gap and tight junctions. Dev Biol 62:415–431
Lee WM, Cran DG, Lane NJ (1982) Carbon dioxide induced disassembly of gap-junctional plaques. J Cell Sci 57:215–228
Makowski L, Caspar DLD, Phillips WC, Goodenough DA (1977) Gap junction structures. II. Analysis of the X-ray diffraction data. J Cell Biol 74:629–645
Merk FB, Albright JT, Botticelli CR (1973) The fine structure of granulosa cell nexuses in rat ovarian follicles. Anat Rec 175:107–125
Pappas GD, Asada Y, Bennett MVL (1971) Morphological correlates of increased coupling resistance at an electrotonic synapse. J Cell Biol 49:173–188
Peracchia C (1977) Gap junctions. Structural changes after uncoupling procedures. J Cell Biol 72:628–641
Peracchia C, Dulhunty AF (1976) Low-resistance junctions in crayfish. Structural changes with functional uncoupling. J Cell Biol 70:419–439
Peracchia C, Mittler BS (1972) Fixation by means of glutaraldehyde hydrogen peroxide reaction products. J Cell Biol 53:234–238
Peracchia C, Peracchia L (1981) Gap junction dynamics: reversible effects of divalent cations. J Cell Biol 87:708–718
Raviola E, Goodenough DA, Raviola G (1980) Structure of rapidly frozen gap junctions. J Cell Biol 87:273–279
Sikerwar S, Malhotra S (1981) Structural correlates of glutaraldehyde induced uncoupling in mouse liver gap junctions. Eur J Cell Biol 25:319–323
Van Harreveld A (1936) A physiological solution for fresh water crustaceans. Proc Soc Exp Biol Med 34:428
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Hanna, R.B., Pappas, G.D. & Bennett, M.V.L. The fine structure of identified electrotonic synapses following increased coupling resistance. Cell Tissue Res. 235, 243–249 (1984). https://doi.org/10.1007/BF00217847
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DOI: https://doi.org/10.1007/BF00217847