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Effect of brief vascular perfusion on the ultrastructure and activity of mitochondria isolated from the rat heart

Cell and Tissue Research volume 240, pages 247–249 (1985)Cite this article

Summary

Mitochondria isolated from heart tissue after a 1-min perfusion with Hanks medium were found to have significantly lower rates of State-3 respiration and respiratory control ratios compared to mitochondria isolated from non-perfused hearts. Examination of the mitochondrial preparations by electron microscopy revealed that a large proportion of the mitochondria isolated from perfused heart tissue were swollen and broken compared to mitochondria from non-perfused hearts.

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Affiliations

  1. Departments of Chemistry and Biological Sciences, Illinois State University, Normal, Ill, USA

    J. L. Staecker, M. J. Nadakavukaren &  A. Richardson

Authors
  1. J. L. Staecker
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  2. M. J. Nadakavukaren
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  3. A. Richardson
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Staecker, J.L., Nadakavukaren, M.J. & Richardson, A. Effect of brief vascular perfusion on the ultrastructure and activity of mitochondria isolated from the rat heart. Cell Tissue Res. 240, 247–249 (1985). https://doi.org/10.1007/BF00217581

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  • DOI: https://doi.org/10.1007/BF00217581

Key words

  • Heart
  • Mitochondria
  • Perfusion
  • Ultrastructure
  • Rat