Cell and Tissue Research

, Volume 234, Issue 1, pp 193–200 | Cite as

Electron microscopy of extracellular materials during the development of a sea star, Patiria miniata (Echinodermata: Asteroidea)

  • R. Andrew Cameron
  • Nicholas D. Holland
Article

Summary

The fine structure of conspicuous extracellular materials during the life history of a sea star (Patiria miniata) is described. The outer surface of the developing sea star is covered by two morphologically different cuticles that appear sequentially during ontogeny. The primary cuticle, which is about 120 nm thick and two-layered, is present from mid-blastula through the end of the larval stage. The secondary cuticle, which is about 1 μm thick and three-layered, first appears on the epidermis of the rudiment region of the larva and, after metamorphosis, covers the entire epidermis of the juvenile and adult stages. During ontogeny, there are only two conspicuous gut cuticles: the first lines the newly invaginated archenteron at the start of the gastrula stage, and the second lines the esophagus during the larval stage. A blastocoelic basal lamina first appears at mid-blastula and persists as subectodermal and subendodermal basal laminae. Ruthenium red-positive granules are detectable between the lateral surfaces of adjacent ectodermal cells during part of the gastrula stage; this transient intercellular material may possibly aid in lateral adhesion between cells.

Key words

Sea star Development Cuticle Extracellular materials Ultrastructure 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • R. Andrew Cameron
    • 1
  • Nicholas D. Holland
    • 2
  1. 1.Center for Coastal Marine Studies, University of California at Santa CruzSanta CruzUSA
  2. 2.Marine Biology Research DivisionScripps Institution of OceanographyLa JollaUSA
  3. 3.Department of Marine SciencesUniversity of Puerto RicoMayaguezUSA

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