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Polymorphism in human IL-1 receptor antagonist gene intron 2 is caused by variable numbers of an 86-bp tandem repeat

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Abstract

We have investigated the polymorphism in intron 2 of the interleukin-1 receptor antagonist gene and identified two new alleles of the system. We have shown that the polymorphism is caused by the variable copy number of an 86-bp sequence, by using the polymerase chain reaction and primers immediately flanking the repeat region, and by direct sequencing. The repeat region contains three potential protein-binding sites and therefore the variable copy number may have functional significance.

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Authors and Affiliations

  1. Section of Molecular Medicine, Department of Medicine, University of Sheffield, Royal Hallamshire Hospital, Sheffield, UK

    Joanna K. Tarlow, Alexandra I. F. Blakemore & Gordon W. Duff

  2. Yamanouchi Research Institute (UK), Oxford, UK

    Andrew Lennard & Roberto Solari

  3. Department of Biological Sciences, The Manchester Metropolitan University, Manchester, UK

    Howard N. Hughes

  4. MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford, UK

    Alexander Steinkasserer

Authors
  1. Joanna K. Tarlow
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  2. Alexandra I. F. Blakemore
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  3. Andrew Lennard
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  4. Roberto Solari
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  5. Howard N. Hughes
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  6. Alexander Steinkasserer
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  7. Gordon W. Duff
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Tarlow, J.K., Blakemore, A.I.F., Lennard, A. et al. Polymorphism in human IL-1 receptor antagonist gene intron 2 is caused by variable numbers of an 86-bp tandem repeat. Hum Genet 91, 403–404 (1993). https://doi.org/10.1007/BF00217368

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  • DOI: https://doi.org/10.1007/BF00217368

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