Summary
The stomach, small intestine and large intestine of the toad, Bufo marinus, were processed for formaldehyde-induced fluorescence histochemistry. After extrinsic denervation or pretreatment with 6-hydroxydopamine to remove catecholamine fluorescence, yellow fluorescence typical of 5-hydroxytryptamine was observed in neurones in the small intestine only. The cell bodies and their processes were confined to the myenteric plexus. Additional pretreatment with 5-hydroxytryptamine enhanced the fluorescence of neurones in the small intestine and revealed yellowfluorescent nerve fibres, but not cell bodies, in the longitudinal and circular muscle layers and myenteric plexus of the large intestine. No fluorescent neurones were observed in the stomach. Following reserpine treatment, which removed native yellow fluorescence in the small intestine, exposure to 5-hydroxytryptophan produced yellow fluorescence in axons in both small and large intestine; exposure to tryptophan never restored fluorescence. The neurotoxin, 5,7-dihydroxytryptamine had no effect on the distribution of yellow-fluorescent neurones in the small and large intestine. No 5-HT-containing mast cells were present in either the small or large intestine. Thin layer chromatography with three different mobile phases showed a 5-hydroxytryptamine-like compound in extracts of mucosa-free small and large intestine but not of stomach.
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References
Anderson C (1983) Evidence for 5-HT-containing intrinsic neurons in the teleost intestine. Cell Tissue Res 185:387–397
Baumgarten HG, Björklund A, Lachenmeyer L, Nobin A, Rosengren E (1973) Evidence for the existence of serotonin-, dopamine- and noradrenaline-containing neurons in the gut of Lampetra fluviatilis. Z Zellforsch 141:33–54
Baumgarten HG, Jenner S, Björklund A, Kleem HP, Schlossberger HG (1982) Serotonin neurotoxins. In: Osborne NN (ed), Biology of serotonergic transmission. John Wiley and Sons, Chichester, pp 249–278
Blessing WW, Chalmers JP, Howe PRC (1978) Distribution of catecholamine-containing cell bodies in the rabbit central nervous system. J Comp Neurol 179:407–424
Boadle-Biber MC (1982) Biosynthesis of serotonin. In: Osborne NN (ed), Biology of serotonergic transmission. John Wiley and Sons, Chichester, pp 63–94
Costa M, Furness JB, Cuello AC, Verhofstad AAJ, Steinbusch HWJ, Elde RP (1982) Neurons with 5-hydroxytryptamine-like immunoreactivity in the enteric nervous system: their visualization and reactions to drug treatment. Neuroscience 7:351–364
Furness JB, Costa M (1978) Distribution of intrinsic cell bodies and axons which take up aromatic amines and their precursors in the small intestine of the guinea pig. Cell Tissue Res 188:527–543
Furness JB, Costa M, Wilson AJ (1977) Water soluble fluorophores produced by reactions with aldehyde solutions for the histochemical localization of catechol- and indolethylamines. Histochemistry 52:159–170
Gershon MD (1982) Serotonergic neurotransmission in the gut. Scand J Gastroenterol 17 [Suppl] 71:27–42
Gillard SM, Read JB (1971) Fluorescent histochemical studies on the effects of 6-hydroxydopamine on adrenaline-containing nerves in the toad. Z Zellforsch 118:493–511
Goodrich JT, Bernd P, Sherman D, Gershon MD (1980) Phylogeny of serotonergic neurons. J Comp Neurol 190:15–28
Holmgren S, Nilsson S (1983) Bombesin-, gastrin/CCK-, 5-hydroxytryptamine-, neurotensin-, somatostatin-, and VIP-like immunoreactivity and catecholamine fluorescence in the gut of the elasmobranch, Squalus acanthias. Cell Tissue Res 234:595–613
Honma S (1970) Presence of monoaminergic neurons in the spinal cord and intestine of the lamprey. Arch Histol Jpn 32:383–393
Read JB, Burnstock G (1968a) Comparative histochemical studies of adrenergic nerves in the enteric plexuses of vertebrate large intestine. Comp Biochem Physiol 27:505–512
Read JB, Burnstock G (1968b) Fluorescence histochemical studies of the mucosa of the vertebrate gastrointestinal tract. Histochemie 16:324–332
Sakharov DA, Salimova NB (1980) Serotonin neurons in the peripheral nervous system of the larval lamprey, Lampetra planeri. A histochemical, microspectrofluorimetric and ultrastructural study. Zool Jhb Physiol 84:231–239
Salimova NB, Feher E (1982) Innervation of the alimentary tract in chondrostean fish (Acipenseridae). A histochemical, microspectrofluorimetric and ultrastructural study. Acta Morphol Acad Sci Hung 30:213–222
Tonelli D, Gattarechia E, Gandolfi M (1982) Thin layer Chromatographic determinations of indolic tryptophan metabolites in human urine using Sep-Pak C18 extraction. J Chromatogr 231:283–290
Watson AHD (1979) Fluorescence histochemistry of the teleost gut: Evidence for the presence of serotonergic neurons. Cell Tissue Res 197:155–164
Wong WC, Sit KH, Ng KKF, Chin KN (1971) The submucous plexus in the small intestine of the toad (Bufo melanostictus). Acta Anat 79:60–69
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Anderson, C., Campbell, G. Evidence for 5-hydroxytryptamine in neurones in the gut of the toad, Bufo marinus . Cell Tissue Res. 238, 313–317 (1984). https://doi.org/10.1007/BF00217303
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DOI: https://doi.org/10.1007/BF00217303