Summary
The ependyma of the IVth ventricle and the central canal of the rat medulla oblongata was investigated using the cytochemical technique for alkaline phosphatase (AlPase) which revealed two types of ependymal cells in the medulla. The central canal type of the ependymal cell occupying the dorsal part of the central canal in the lower medulla exhibited intense AlPase activity with light microscopy. These cells had reaction products in all plasma membranes, including the microvilli and the cilia at the luminal cell surface. Some cells appeared to be tanycytes, since the process reached the basement membrane of the parenchymal blood vessel. The ventricular type of ependymal cells, which form the floor of the IVth ventricle and the central canal, contained no reaction products in any structure of the luminal cell surface.
The possible relationship between the cerebrospinal fluid and the nervous tissues through the ependymal linings is discussed.
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References
Arluison M, Conrath-Verrier M, Tauc M, Mailly P, De la Manche IS, Cesselin F, Bourgoin S, Hamon M (1983) Different localizations of met-enkephalin-like immunoreactivity in rat forebrain and spinal cord using hydrogen peroxide and Triton X-100. Light microscopic study. Brain Res Bull 11:555–571
Bourne GH (1957) Phosphatases in the central nervous system. Nature 179:1247–1248
Brightman MW (1965) The distribution within the brain of ferritin injected into cerebrospinal fluid compartments. I. Ependymal distribution. J Cell Biol 26:99–123
Brightman MW, Palay SL (1963) The fine structure of ependyma in the brain of the rat. J Cell Biol 19:415–439
De Jonge HR, Ghijsen WEJM, Von Os CH (1981) Phosphorylated intermediates of Ca++-ATPase and alkaline phosphatase in plasma membranes from rat duodenal epithelium. Biochem Biophys Acta 647:140–149
Felten DL, Cummings JP, Burnett BT (1981) Ontogeny of caudal fourth ventricle tanycytes in the rabbit brain: A Golgi study. Anat Rec 200:321–330
Felten DL, Harrigan P, Burnett BT, Cummings JP (1981) Fourth ventricular tanycytes: a possible relationship with monoaminergic nuclei. Brain Res Bull 6:427–436
Finley JCW, Maderdrut JL, Petrusz P (1981) The immunocytochemical localization of enkephalin in the central nervous system of the rat. J Comp Neurol 198:541–565
Firth JA, Bock R (1976) Distribution and properties of an adenosine triphosphatase in the tanycyte ependyma of the IIIrd ventricle of the rat. Histochemistry 47:145–157
Fleischhauer K (1972) Ependyma and subependymal layer. In: Bourne GH (ed) The structure and function of nervous system. Academic Press, New York London, pp 1–46
Hirano A, Mastui T, Zimmerman HM (1975) Electron microscopic observation of ependyma. Neurol Surg 3:237–244 (in Japanese)
Hugon J, Borgers M (1968) Fine structural localization of acid and alkaline phosphatase activities in the absorbing cells of the duodenum of rodents. Histochemie 12:42–66
Kaplan MM (1972) Alkaline phosphatase. Gastroenterology 62:452–468
Knight BK, Hayes MMM, Symington RB (1974) Anatomical evidence to support the role of the cerebrospinal fluid as a transmitter medium in the pineal-hypothalamic axis. S Afr J Med Sci 39:25–31
Mayahara H, Hirano H, Saito T, Ogawa K (1967) The new lead citrate method for the ultracytochemical demonstration of ac- tivity of non-specific alkaline phosphatase (orthophosphoric monoester phosphohydrolase). Histochemie 11:88–96
Merchenthaler I, Hynes MA, Vigh S, Shally AV, Petrusz P (1983) Immunocytochemical localization of corticotropin releasing factor (CRF) in the rat spinal cord. Brain Res 275:373–377
Nandy K, Bourne GH (1965) Histochemical studies on the ependyma lining the central canal of the spinal cord in the rat with a note on its functional significance. Acta Anat 60:539–550
Peters A, Palay SL, Webster H deF (1976) The fine structure of the nervous system: The neurons and supporting cells. WB Saunders Camp, Philadelphia London Toronto
Rall DP (1968) Transport through the ependymal linings. Prog Brain Res 29:159–167
Robinson AG, Zimmerman EA (1973) Cerebrospinal fluid and ependymal neurophysin. J Clin Invest 56:1260–1267
Rodríguez EM (1976) The cerebrospinal fluid as a pathway in neuroendocrine integration. J Endocrinol 71:407–443
Sar M, Stumpf WE, Miller RJ, Chang K-J, Cuatrecasas P (1978) Immunohistochemical localization of enkephalin in rat brain and spinal cord. J Comp Neurol 182:17–38
Scott DE, Dudley GK, Knigge KM (1974) The ventricular system in the neuroendocrine mechanisms. II. In vitro monoamine transport by ependyma of the median eminence. Cell Tissue Res 154:1–16
Seitz R, Löhler J, Schwendemann G (1981) Ependyma and meninges of the spinal cord of the mouse. A light- and electronmicroscopic study. Cell Tissue Res 220:61–72
Swarup G, Cohen S, Garbers DL (1981) Selective dephosphorylation of proteins containing phosphoserine by alkaline phosphatase. J Biol Chem 256:8197–8201
Van Deurs B (1980) Structural aspects of brain barriers, with special reference to the permeability of the cerebral endothelium and choroidal epithelium. Int Rev Cytol 65:117–191
Vengesa PB, Hopfer U (1979) Cytochemical localization of alkaline phosphatase and Na+-pump sites in adult rat colon. J Histochem Cytochem 27:1231–1235
Wagner HJ, Pilgrim Ch (1974) Extracellular and transcellular transport of horseradish peroxidase (HRP) through the hypothalamic tanycyte ependyma. Cell Tissue Res 152:477–491
Wood JH (1983) Physiology and Pharmacology of peptides, steroid, and other hormones in cerebrospinal fluid. In: Wood JH (ed) Neurobiology of cerebrospinal fluid 2. Plenum Press, New York London, pp 43–65
Yoshioka T, Inomata K (1983) Cytochemical localization of alkaline phosphatase in the spinal cord ependyma of newborn and adult rats. Acta Histochem Cytochem 16:77–83
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Yoshioka, T., Inomata, K. & Tanaka, O. Cytochemical localization of alkaline phosphatase in the ependyma of the rat medulla oblongata. Cell Tissue Res. 241, 415–420 (1985). https://doi.org/10.1007/BF00217188
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DOI: https://doi.org/10.1007/BF00217188