Summary
The secretory dynamics of the Crustacean Hyperglycemic Hormone (CHH)-producing cells in the eyestalk of the crayfish Astacus leptodactylus were studied during the daily cycle (12 h light/12 h dark). The different secretory stages of individual cells were determined by means of immunocytochemistry combined with morphometric analysis at the light-microscopic level. The data obtained were correlated with the 24-h rhythmicity of blood glucose concentration. The results suggest the following hypothesis. The synthetic activity of the CHH cells receives a stimulus 2 h before the beginning of the dark period, resulting in a pronounced transfer of CHH granules into the axons. These CHH granules reach the axon terminals after the onset of the dark period. At that time a burst of exocytotic activity occurs, causing a strong release of CHH into the hemolymph. Four hours later this CHH release results in hyperglycemia. The same process, though with less intensity, is repeated and causes a second smaller glucose peak at the beginning of the light period.
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Chandra Sehara Reddy D, Raghupathi M, Purusotham KR, Naidu BP (1981) Daily rhythms in levels of blood glucose and hepatopancreatic glycogen in the freshwater field crab Oziotelphusa senex senex (Fabricius). Ind J Exp Biol 19:403–404
Drach P (1944) Etude préliminaire sur le cycle d'intermue et son conditionnement hormonal chez Leander serratus (Pennant). Bull Biol Fr Belg 78:40–62
Gorgels-Kallen JL, Van Herp F (1981) Localization of crustacean hyperglycemic hormone (CHH) in the X-organ sinus gland complex in the eyestalk of the crayfish, Astacus leptodactylus (Nordmann, 1842). J Morphol 170:347–355
Gorgels-Kallen JL, Van Herp F (1982) Localization and characterization of the crustacean hyperglycemic hormone-producing cells in the eyestalk of decapod Crustacea. J Endocrinol 94:(Suppl)55P, 109 (abstract)
Gorgels-Kallen JL, Voorter CEM (1984) Secretory stages of individual CHH-producing cells in the eyestalk of the crayfish Astacus leptodactylus, determined by means of immunocytochemistry. Cell Tissue Res 237:291–298
Gorgels-Kallen JL, Van Herp F, Leuven RSEW (1982) A comparative immunocytochemical investigation of the crustacean hyperglycemic hormone (CHH) in the eyestalk of some decapod crustacea. J Morphol 174:161–168
Hamann A (1974) Die neuroendokrine Steuerung tagesrhythmischer Blutzuckerschwankungen durch die Sinusdrüse beim Flusskrebs. J Comp Physiol 89:197–214
Jaros PP (1977) Untersuchungen zur Histologie und Physiologie der Neurosekretion in den optischen Ganglien des Flusskrebses Orconectes limosus. Thesis Universität Ulm (MNH), Germany, 93 pp
Jaros PP (1979) Immunocytochemical demonstration of the neurosecretory X-organ complex in the eyestalk of the crab Carcinus maenas. Histochemistry 63:303–310
Jaros PP (1980a) Axonal transport of neurosecretory material in the X-organ sinus gland tract of the crayfish Orconectes limosus. Verh Dtsch Zool Ges, p 348
Jaros PP (1980) Cellular rhythmicity of RNA and protein synthesis in the crustacean hyperglycemic hormone producing neurosecretory perikarya of the X-organ studied by autoradiography. Eur J Cell Biol 22:499
Jaros PP, Keller R (1979) Immunocytochemical identification of hyperglycemic hormone-producing cells in the eyestalk of Carcinus maenas. Cell Tissue Res 204:379–385
Kleinholz LH, Keller R (1979) Endocrine regulation in Crustacea. In: Barrington EJW (ed) Hormones and evolution. Academic Press, New York, vol 1, pp 160–213
Luft JH (1961) Improvements in epoxy resin embedding sections. J Biophys Biochem Cytol 9:409–414
Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212
Sternberger LA (1974) Immunocytochemistry. Prentice-Hall, Inc., Englewood Cliffs, New Jersey, p 246
Strolenberg GECM (1979) Functional aspects of the sinus gland in the neurosecretory system of the crayfish Astacus leptodactylus. An ultrastructural approach. Thesis Catholic University Nijmegen, The Netherlands, p 140
Strolenberg GECM, Van Herp F (1977) Mise en évidence du phénomène d'exocytose dans la glande du sinus d'Astacus leptodactylus (Nordmann) sous l'influence d'injections de sérotonine. C R Acad Sci D 284:57–60
Strolenberg GECM, Van Helden HPM, Van Herp F (1977) The ultrastructure of the sinus gland of the crayfish Astacus leptodactylus (Nordmann). Cell Tissue Res 180:203–210
Van Herp F, Strolenberg GECM (1980) Functional aspects of the neurosecretory system in the eyestalk of the crayfish Astacus leptodactylus with special reference to the hyperglycemic hormone. Gen Comp Endocrinol 40:364 (abstract)
Van Herp F, Van Buggenum HJM (1979) Immunocytochemical localization of hyperglycemic hormone (HGH) in the neurosecretory system of the eyestalk of the crayfish Astacus laptodactylus. Experientia 35:1527–1528
Van Herp F, Van Wormhoudt A, Van Venrooy WAJ, BellonHumbert C (1984) Immunocytochemical study of Crustacean Hyperglycemic Hormone (CHH) in the eyestalks of the prawn Palaemon serratus (Pennant) and some other Palaemonidae, in relation to variations in the blood glucose level. J Morphol (in press)
Weibel ER (1979) Stereological methods. Practical methods for biological morphometry. Academic Press, London, vol 1, p 415
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Gorgels-Kallen, J.L., Voorter, C.E.M. The secretory dynamics of the CHH-producing cell group in the eyestalk of the crayfish, Astacus leptodactylus, in the course of the day/night cycle. Cell Tissue Res. 241, 361–366 (1985). https://doi.org/10.1007/BF00217181
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DOI: https://doi.org/10.1007/BF00217181