Summary
Most studies on human sperm chromosomes from normal men involve the heterologous fertilization of zona free hamster eggs by unselected human sperm. In this work, we have performed cytogenetic studies of highly motile sperm, selected by a swim-up method. A total of 505 motile human sperm complements from three normal donors was analysed. The total frequency of sperm with chromosomal abnormalities (10.9%; 6.9% structural aberrations and 4.0% aneuploidy) and the sex ratio (50.4% X∶49.6% Y) were similar to those obtained from whole semen samples. Our results indicate that the selection of motile sperm does not imply chromosomal selection.
Similar content being viewed by others
References
Andolz P, Bielsa MA, Genescà A, Benet J, Marina S, Egozcue J (1987) Improvement of sperm quality in abnormal semen samples using a modified swim-up procedure. Hum Reprod 2:99–101
Barbi G, Steinbach P, Vogel W (1984) Nonrandom distribution of methotrexate-induced aberrations on human chromosomes. Detection of further folic acid sensitive fragile site. Hum Genet 68:290–294
Benet J, Genescà A, Navarro J, Egozcue J, Templado C (1986) Gbanding of human sperm chromosomes. Hum Genet 73:181–182
Benet J, Fuster C, Genescà A, Navarro J, Miró R, Egozcue J, Templado C (1989) Expression of fragile sites in human sperm and lymphocyte chromosomes. Hum Genet 81:239–242
Benet J, Navarro J, Genescà A, Egozcue J, Templado C (1991) Chromosome abnormalities in human sperm after albumin or TEST-Yolk capacitation. Human Reprod 6:369–375
Brandriff BF, Carrano AV (1987) Nondisjuntion: studies with spermatozoa. In: Vig BK, Sandberg AA (eds) Aneuplody, part A: Incidence and etiology. Liss, New York, pp 51–65
Brandriff B, Gordon L, Ashworth L, Watchmaker G, Moore D II, Wyrobek AJ, Carrano AV (1985) Chromosomes of human sperm: variability among normal individuals. Hum Genet 70:18–24
Brandriff BF, Gordon LA, Haendel S, Ashworth LK, Carrano AV (1986) The chromosomal constitution of human sperm selected for motility. Fertil Steril 46:686–690
Brandriff B, Gordon LA, Moore II D, Carrano AV (1988) An analysis of structural aberrations in human sperm chromosomes. Cytogenet Cell Genet 47:29–36
Brandriff BF, Gordon LA, Carrano AV (1990) Cytogenetics of human sperm: structural aberrations and DNA replication. In: Mendelsohn ML, Albertini RJ (eds) Mutation and the environment, part B. Wiley Liss, New York, pp 425–434
Egozcue J, Templado C, Caballín MR, Navarro J, Miró R, Benet J, Genescà A (1989) Genetics of human spermatozoa. Proceedings of XIII World Congress on Fertility and Sterility, Marrakesh 1989, pp 608
Estop A, Català V, Santaló J (1990) Chromosome constitution of highly motile mouse sperm. Mol Reprod Dev 27:168–172
Ford JH (1987) Chromosome displacement hypothesis. In: Vig BK, Sandberg AA (ed) Aneuploidy, part A: Incidence and etiology. Liss, New York, pp 287–248
Fuster C, Miró R, Templado C, Barrios L, Egozcue J (1989) Expression of folate-sensitive fragile sites in lymphocyte chromosomes. Hum Genet 81:243–246
Genescà A, Miró R, Caballín MR, Benet J, Navarro J, Templado C, Bonfill X, Egozcue J (1987) Expression of a possible constitucional “hot spot” in sperm chromosomes of a patient treated for wilms' tumor. Cancer Genet Cytogenet 29:91–96
ISCN (1985) An international system for human cytogenetic nomenclature. Harnden DG, Klinger HP (eds) Published in collaboration with Gytogenet Cell Genet. Karger, Basel
Jenderny J, Röhrborn G (1987) Chromosome analysis of human sperm. I. First results with a modified method. Hum Genet 76:385–388
Kamiguchi Y, Mikamo K (1986) An improved, efficient method for analyzing human sperm chromosomes using zona-free hamster ova. Am J Hum Genet 38:724–740
Martin RH, Rademaker A (1990) The frequency of aneuploidy individual chromosomes in 6821 human sperm chromosome complements. Cytogenet Cell Genet 53:103–107
Martin RH, Balkan W, Burns K, Rademaker AW, Lin CC, Rudd NL (1983) The chromosome constitution of 1000 human spermatozoa. Hum Genet 63:305–309
Martin RH, Rademaker AW, Hildebrand K, Long-Simpson L, Peterson D, Yamamoto J (1987) Variation in the frequency and type of sperm chromosomal abnormalities among normal men. Hum Genet 77:108–114
Martin RH, Templado C, Ko E, Rademaker A (1990) Effect of culture conditions and media on the frequency of chromosomal abnormalities in human sperm chromosome complements. Mol Reprod Dev 26:101–104
Pellestor F (1988) Recherche sur les chromosomes des gamètes de l'espèce humaine. PhD thesis. Université Scientifique et Médicale, Grenoble
Rao PN, Heerema NA, Palmer CG (1988) Fragile sites induced by FUdR, caffeine, and aphidicolin. Their frequency, distribution and analysis. Hum Genet 78:21–26
Rudak R, Jacobs PA, Yanagimachi R (1978) Direct analysis of the chromosome constitution of human spermatozoa. Nature 274:911–913
Sutherland GR, Ledbetter DH (1989) Report of the committee on cytogenetic markers. (10th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 51:452–458
Tarkowski AK (1966) An air drying method for chromosome preparation from mouse eggs. Cytogenetics 5:394–400
Templado C, Benet J, Genescà A, Navarro J, Caballín MR, Miró R, Egozcue J (1988) Human sperm chromosomes. Hum Reprod 3:133–138
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Benet, J., Genescà, A., Navarro, J. et al. Cytogenetic studies in motile sperm from normal men. Hum Genet 89, 176–180 (1992). https://doi.org/10.1007/BF00217119
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00217119