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Immunohistochemical localization of vasoactive intestinal polypeptide(VIP)-containing neurons in the hypothalamus of the Japanese quail, Coturnix coturnix

Cell and Tissue Research volume 226, pages 13–26 (1982)Cite this article

Summary

The localization of vasoactive intestinal polypeptide (VIP) in the hypothalamus of the quail has been studied by means of light- and electron-microscopic immunohistochemistry. Numerous VIP-immunoreactive perikarya are distributed in the caudal portion of the nucleus infundibularis (n. tuberis) and nucleus mamillaris lateralis, and sparse in the preoptic area, nucleus supraopticus and nucleus paraventricularis. Dense localization of immunoreactive-VIP fibers is observed in the external layer of the median eminence, in close contact with the primary portal capillaries. The main origins of these fiber terminals are VIP-immunoreactive perikarya of the nucleus infundibularis. These neurons are spindle or bipolar and extend one process to the ventricular surface and another to the external layer of median eminence. They are CSF-contacting neurons and apparently constitute the tubero-hypophysial tract that links the third ventricle and the hypophysial portal circulation. VIP-reactive neurons in the nucleus mamillaris lateralis also project axons to the external layer of the median eminence, constituting the posterior bundle of the tuberohypophysial tract. Numerous VIP-immunoreactive perikarya occur also in the nucleus accumbens/pars posterior close to the lateral ventricle. They are also CSF-contacting neurons extending a process to the lateral ventricle. There are moderate distributions of VIP-reactive fibers in the area ventralis and in the area septalis.

Ultrastructurally, the immunoreactive products against VIP are found in the elementary granules, 75–115 nm in diameter, within the nerve fibers in the median eminence.

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Affiliations

  1. Department of Veterinary Anatomy, Iwate University, Morioka, Japan

    Shizuhiro Yamada &  Shin-ichi Mikami

  2. Laboratory of Bioorganic Chemistry, Shizuoka College of Pharmacy, Shizuoka, Japan

    Noboru Yanaihara

Authors
  1. Shizuhiro Yamada
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  2. Shin-ichi Mikami
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  3. Noboru Yanaihara
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Additional information

This investigation was supported by Scientific Research Grants No. 00556196, No. 56360027 and No. 56760183 from the Ministry of Education of Japan to Professor Mikami and Mr. Yamada

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Yamada, S., Mikami, Si. & Yanaihara, N. Immunohistochemical localization of vasoactive intestinal polypeptide(VIP)-containing neurons in the hypothalamus of the Japanese quail, Coturnix coturnix . Cell Tissue Res. 226, 13–26 (1982). https://doi.org/10.1007/BF00217078

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  • DOI: https://doi.org/10.1007/BF00217078

Keywords

  • Hypothalamus
  • Vasoactive intestinal polypeptide (VIP)
  • Immunohistochemistry
  • Japanese quail
  • Tuberohypophysial system