Summary
An autoradiographic study was performed on the effects of hydrocortisone, reserpine, propranolol and phentolamine on the uptake of tritiated amines by adrenal medullary cells of the mouse. Oral feeding of hydrocortisone had no significant effect on the normal uptake pattern of dopamine, noradrenaline or adrenaline by medullary cells of different type (A cells or NA cells) or location (marginal or central), although the overall amounts taken up were markedly reduced. Handling the animals led to similar reductions in the uptake of all three amines and was thus clearly shown to be the important factor in this effect. Reserpine reduced the uptake of [3H] noradrenaline to 25 % of the control value although the relative distribution remained unchanged. Propranolol and phentolamine had no observed effect on [3H] noradrenaline uptake. These results are discussed in the light of the previously reported action of ACTH in reversing the effects of hypophysectomy on medullary amine uptake (Hirano and Kobayashi 1978), and it is concluded that ACTH must exert this effect directly on the adrenal medulla rather than through the secretion of adrenal corticosteroids. It is also suggested that reserpine acts, as in neurons, by blocking amine uptake into intracellular granules rather than by blocking uptake into the cell itself.
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References
Bertler Å, Hillarp N-Å, Rosengren E (1960) Some observations on the synthesis and storage of catecholamines in the adrenaline cells of the suprarenal medulla. Acta Physiol Scand 50:124–131
Bertler Å, Hillarp N-Å, Rosengren E (1961) Effect of reserpine on the storage of new formed catecholamines in the adrenal medulla. Acta Physiol Scand 52:44–48
Budd GC, Salpeter MM (1969) The distribution of labeled norepinephrine within sympathetic nerve terminals studied with electron microscopic radioautography. J Cell Biol 41:21–32
Callingham BA, Mann M (1958) Replacement of adrenaline and noradrenaline in the innervated and denervated adrenal gland of the rat following depletion with reserpine. Nature 182:1020–1021
Callingham BA, Mann M (1962) Depletion and replacement of the adrenaline and noradrenaline contents of the rat adrenal gland, following treatment with reserpine. Br J Pharmacol 18:139–149
Carlsson A, Hillarp N-Å, Waldeck B (1962) A Mg+ + -ATP dependent storage mechanism in the amine granules of the adrenal medulla. J Biol Chem 227:243–252
Carlsson A, Hillarp N-Å, Waldeck B (1963) Analysis of the Mg+ +-ATP dependent storage mechanism in amine granules of the adrenal medulla. Acta Physiol Scand 59, (Suppl) 215:1–38
Coupland RE (1968) Corticosterone and methylation of noradrenaline by extra-adrenal chromaffin tissue. J Endocrinol 41:487–490
Coupland RE, MacDougall JDB (1966) Adrenaline formation in noradrenaline-storing chromaffin cells in vivo induced by corticosterone. J Endocrinol 36:317–324
Dahlström A, Fuxe K, Hillarp N-Å (1965) Site of action of reserpine. Acta Pharmacol Toxicol (KBH) 22:277–292
Eränkö L, Eränkö O (1972) Effect of hydrocortisone on histochemically demonstrable catecholamines in the sympathetic ganglia and extra-adrenal chromaffin tissue of the rat. Acta Physiol Scand 84:125–133
Eränkö O, Lempinen M, Räisänen L (1966) Adrenaline and noradrenaline in the organ of Zuckerkandl and adrenals of newborn rats treated with hydrocortisone. Acta Physiol Scand 66:253–254
Giachetti A, Hollenbeck RA, Shore PA (1974) Localization and binding of reserpine in the membrane of adrenomedullary amine storage granules. Naunyn Schmiedeberg Arch Pharmacol 283:263–275
Graham LA (1966) Effect of phenoxybenzamine and hydergine on urinary catecholamines in rats during restraint. Acta Physiol. Scand 68:18–22
Hirano T, Kobayashi S (1978) The effects of hypophysectomy on the uptake and distribution of [3H] dopamine in the mouse adrenal medulla: an autoradiographic study. Arch Histol Jpn 41:401–410
Hirano T, Kobayashi S (1980) Stress effects on the localization of exogenous [3H] dopamine in the mouse adrenal medulla. Brain Res 192:281–286
Hofmann FG (1956) Observations on in vitro adrenal steroid synthesis in the albino mouse. Endocrinology 59:712–715
Kent C, Coupland RE (1981) On the uptake of exogenous catecholamines by adrenal chromaffin cells and nerve endings. Cell Tissue Res 221:371–383
Kley H, Herberg L, Kruskemper H (1976) Evaluation of adrenal function in mice by measurement of urinary excretion of free corticosteroids. J Steroid Biochem 7:138–185
Kvetňanský R, Mikulaj L (1970) Adrenal and urinary catecholamines in rats during adaptation to repeated immobilization stress. Endocrinology 87:738–743
Leduc J (1961) Catecholamine production and release in exposure and acclimation to cold. Acta Physiol Scand (Suppl) 53:183 1–101.
Lempinen M (1964) Extra-adrenal chromaffin tissue of the rat and the effect of cortical hormones on it. Acta Physiol Scand (Suppl) 62:231 1–91
Sugawara K, Takami N, Maemura S, Niwa M, Ozaki M (1980) β-Adrenoceptor blocking agents release catecholamines from rat adrenal medulla. Eur J Pharmacol 62:287–295
Volina EV (1977) Some physiological mechanisms of the regulation of noradrenaline-H3 incorporation into the adrenal glands of rats. Problem Endokrinology (Moskva) 23:87–93
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Kent, C., Monkhouse, W.S. & Coupland, R.E. Effect of hydrocortisone, reserpine, propranolol and phentolamine on in vivo uptake of exogenous amines by adrenal chromaffin cells. Cell Tissue Res. 221, 385–393 (1981). https://doi.org/10.1007/BF00216742
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DOI: https://doi.org/10.1007/BF00216742