Summary
Human pituitary adenomas proliferate neither in cell culture nor in athymic “nude” mice. We propose that one or several of the humoral factors necessary for the growth of pituitary adenomas is missing in these experimental environments. The purpose of our experiments was to examine the possible influence of the hypothalamus in supporting cellular proliferation, and thus adenoma growth. Fragments from four human pituitary adenomas (three pituitary prolactinomas; one ACTH-secreting adenoma) were transplanted into the pituitary fossa of total-body irradiated, hypophysectomized rats. The rats were killed after two weeks and perfused with a mixture of formalin and India ink. Histologic examination of serial sagittal sections through the pituitary fossa and the adjacent brain showed: vascularization of the grafts from the pituitary stalk and from the scar tissue in the sphenoid bone; survival of some adenomas; and numerous mitoses in an ACTH-secreting specimen obtained from a patient who had Cushing's disease. We conclude from these experiments that as yet unidentified hypothalamic factors are essential for the growth of certain types of pituitary adenomas.
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Batzdorf U, Gold V, Mathews N, Brown J (1971) Human growth hormone in cultures of human pituitary tumors. J Neurosurg 34:741–748
Bullard DE, Bigner DE (1979) The use of athymic “nude” mice for the in vivo study of human nervous system tumors. J Neuropathol Exp Neurol 38:306
Harris GW (1950) Oestrous rhythm. Pseudopregnancy and the pituitary stalk in the rat. J Physiol 111:347–360
Harris GW (1955) Neural control of the pituitary gland. Edward Arnold, London, pp 46–59
Harris GW, Jacobsohn D (1952) Functional grafts of the anterior pituitary gland. Proc R Soc Lond (Biol) 139:263–276
Kameya T, Shimosato Y, Hayashi H (1977) Growth and differentiation of hormone-producing human tumors in the nude mice. In: Nomura T, Ohsawa N, Tamaoki N, Fujiwara K (eds) Proceedings of the Second International Workshop on Nude Mice, Stuttart, G Fischer Verlag, New York, pp 405–416
Landolt AM (1973) Regeneration of the human pituitary. J Neurosurg 39:35–41
Landolt AM (1980) Biology of pituitary adenomas. In: Faglia G, Giovanelli MA, McLeod RM (eds) Pituitary Microadenomas, Proceedings of the Serono Symposia (vol 29). Academic Press, London, New York, San Francisco, pp 107–109
Landolt AM, Kistler GS, Barker M, Deen D, Wilson CB (1980) Transplantation of human pituitary adenomas into experimental animals. In: Derome PJ, Jedynak CP, Peillon F (eds) Pituitary Adenomas: Biology, physiopathology and treatment — Second European Workshop. Asclepios Publishers, Paris, pp 49–57
Lipson LG, Beitins IZ, Kornblith PL, McArthur JW, Frieson HG, Kliman B, Kjellberg RN (1979) Tissue culture studies of human pituitary tumours: Long term release of anterior pituitary hormones into the culture medium. Acta Endocrinol (Kbh) 90:421–423
Magoun HW, Fisher C, Ranson SW (1939) The neurohypophysis and water exchange in the monkey. Endocrinology 25:161–174
Ohsawa N, Ueyama Y, Morita K (1977) Heterotransplantation of human functioning tumors to nude mice. In: Nomura T, Ohsawa N, Tamaoki K, Fujiwara K (eds) Proceedings of the Second International Workshop on Nude Mice. G Fischer Verlag, Stuttgart, New York, pp 395–404
O'Sullivan JP, Alexander KM, Jenkins JS (1978) Maintenance of functioning human pituitary tumours in “nude” athymic mice. J Endocrinol 79:139–140
Peillon F, Cesselin F, Garnier PE, Brandi AM, Donnadieu M, L'Hermite M, Dubois MP (1978) Prolactin secretion and synthesis in short- and long-term organ culture of pituitary tumours from acromegalic patients. Acta Endocrinol (Kbh) 87:701–715
Saeger W, (1977) Die Morphologie der paraadenomatösen Adenohypophyse. Virchows Arch (Pathol Anat) 372:299–314
Sato GH (1975) The role of serum in cell culture. In: Litwack G (ed) Biochemical actions of hormones, Academic Press, New York, Vol 3, pp 391–396
Sato GH, Reid L (1978) The replacement of serum in cell culture by hormones. Int Rev Biochem 20:219–251
Shimosato Y (1977) List of human tumors transplanted to nude mice. In: Nomura T, Ohsawa N, Tamaoki N, Fujiwara K (eds) Proceedings of the Second International Workshop on Nude Mice, Stuttgart, G Fischer Verlag, New York, pp 590–591
Shin S, Reid LC, Colburn PC (1977) Development of transplantable functional endocrine tumor lines in nude mice. In: Nomura T, Ohsawa N, Tamaoki N, Fujiwara K (eds) Proceedings of the Second International Workshop on Nude Mice, Stuttgart, G Fischer Verlag, New York, pp 375–393
Smith PE (1930) Hypophysectomy and replacement therapy in the rat. Am J Anat 45:205–273
Sweet WH, Field RA (1968) Pituitary stalk section for angiopathic diabetic retinopathy: technique, mortality, morbidity, postoperative management. In: Goldberg MF, Fine SL (eds) Symposium on the treatment of diabetic retinopathy. US Department of Health, Education, and Welfare, Washington DC, pp 355–364
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This study was supported by a grant to Dr. Landolt from the Jubiläumsspende of the University of Zürich, Switzerland.
The results were presented in part at the Second European Workshop on Pituitary Adenomas, Paris, September 20–22, 1979
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Landolt, A.M., Barker, M., Deen, D.F. et al. Subhypothalamic grafts of human pituitary adenomas in total-body irradiated rats. Cell Tissue Res. 221, 269–277 (1981). https://doi.org/10.1007/BF00216731
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DOI: https://doi.org/10.1007/BF00216731