Abstract
The HLA-A9 family has been characterized as possessing two well defined specificities; HLA-A23 and A24. Serological studies have suggested the presence of a third member of this family HLA-A9.3, however there is doubt surrounding the existence of this specificity. HLA-A23, A24, and the putative A9.3 proteins were analyzed biochemically by immunoprecipitation and isoelectric focusing. Both HLA-A24 and A9.3 have identical isoelectric points whereas A23 is different. We have sequenced cDNA encoding HLA-A23, A24, and A9.3. From the observed protein sequences, we found A9.3 to differ from A24 by two amino acid substitutions located in the α2 helix of the class I molecule. These substitutions are expected to significantly change the shape of the peptide binding cleft.
References
Bjorkman, P. J. and Parham, P.: Structure, function, and diversity of class I major histocompatibility complex molecules. Annu Rev Biochem 59: 253–258, 1990
Bodmer, J. G.: HLA-A9. In J. Dausset and J. Colombani (eds.): Histocompatibility Testing 1972, p 637, Munksgaard, Copenhagen, 1972
Bodmer, J. G.: The ABC of HLA. In F. Kissmeyer-Nielsen (ed.): Histocompatibility Testing 1975, p 21, Munksgaard, Copenhagen, 1975
Cheung, K. W. K., King, L. S., Coffing, M. J. N. M. N., Boral, L. I., Oldfather, J. W., Chen, S., Strothman, R., Hsia, S., Johnson, U., Clarkholm, B., Low, B., Yang, E. K., Merrart, H., Masniuk, J., Rush, D., Perrier, P., Marwick, J., Ye, G., Middleton, D., Good, R. A., Benson, J. K., Carpenter, C. B., Belcher, R., Lin, J., and Riley, M.: Antigen Society report 1 (A1, Aw36, A3, A11, A9, A23, A24). In B. Dupont (ed.): Immunology of HLA, Vol I: Histocompatibility Testing 1987, pp. 116–119, Springer, New York, 1989
Cowan, E. P., Jelachich, M. L., Biddison, W. E., and Coligan, J. E.: DNA sequence of HLA-A11: remarkable homology with HLA-A3 allows identification of residues involved in epitopes recognized by antibodies and T cells. Immunogenetics 25: 241–250, 1987
Dausset, J.: The genetics of transplantation antigens. Transplant Proc 3: 8–14, 1971
Ennis, P. D., Zemmour, J., Salter, R. D., and Parham, P.: Rapid cloning of HLA-A, B cDNA by using the polymerase chain reaction: Frequency and nature of errors produced in amplification. Proc Natl Acad Sci USA 87: 2833–2837, 1990
Garrett, T. P. J., Saper, M. A., Bjorkman, P. J., Strominger, J. L., and Wiley, D. C.: Specificity pockets for the side chains of peptide antigens in HLA-Aw68. Nature 342: 692–696, 1989
Kato, K., Trapani, J. A., Allopenna, J., Dupont, B., and Yang, S. Y.: Molecular analysis of the serologically defined HLA-Aw19 antigens. J Immunol 143: 3371–3378, 1989
Lau, M. and Terasaki, P. I.: Detection of new HLA-ABC variants through the International cell exchange, 1988–1989. Abstract 15th Annual ASHI Meeting 1989. Hum Immunol: 3, 1989
Neefies, J. J., Breur-Vriesendorp, B. S., van Seventer, G. A., Ivanyi, P., Ploegh, H. L.: An improved biochemical method for the analysis of HLA-class I antigens. Hum Immunol 16: 169–181, 1986
N'Guyen, C., Sodoyer, R., Trucy, J., Strachan, T., and Jordan, B. R.: The HLA-Aw24 gene: sequence, surroundings and comparison with the HLA-A2 and HLA-A3 genes. Immunogenetics 21: 479–489, 1985
Pease, L. R., Horton, R. M., Pullen, J. K., and Cai, Z.: Structure and diversity of class I antigen presenting molecules in the mouse. Crit Rev Immunol 11: 1–32, 1991
WHO Committee for factors of the HLA system: Nomenclature for factors of the HLA system, 1987. Immunogenetics 28: 391–398, 1988
Yang, S. Y.: A standardized method for detection of HLA-A and HLA-B alleles by one-dimensional isoelectric focusing (IEF) gel electrophoresis. In B. Dupont (ed.): Immunobiology of HLA, Vol I: Histocompatibility Testing 1987, pp. 332–335, Springer, New York, 1989a
Yang, S. Y.: Population analysis of class I HLA antigens by one-dimensional isoelectric focusing gel electrophoresis: Workshop Summary Report. In B. Dupont (ed.): Immunobiology of HLA, vol I: Histocompatibility Testing 1987, pp. 309–331, Springer, New York, 1989b
Zemmour, J., Koller, B. H., Ennis, P. D., Geraghty, D. E., Lawlor, D. A., Orr, H. T., and Parham, P.: HLA-AR, an inactivated antigen-presenting locus related to HLA-A. J Immunol 144: 3619–3629, 1990
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The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers M64740 (HLA-A *2402); M64741 (HLA-A *2403); M64742 (HLA-A *2301).
Address correspondence and offprint requests to: P. Parham.
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Little, AM., Madrigal, J.A. & Parham, P. Molecular definition of an elusive third HLA-A9 molecule: HLA-A9.3. Immunogenetics 35, 41–45 (1992). https://doi.org/10.1007/BF00216625
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DOI: https://doi.org/10.1007/BF00216625