Summary
Cells from the dog's tracheal mucosa formed confluent epithelial sheets in culture. Typical tight junctions separated the apical from the basolateral portion of the cell membrane. The apical portion of the cell contained numerous short microvilli and a pronounced glycocalyx. The basolateral portion of the plasma membrane was unspecialized except for extensive gap junctions between cells. Freeze-fracture showed that the cultured cells lacked the basolateral square (orthogonal) arrays of the original tissue, particles previously implicated in ion transport. Formation of domes indicated the presence of active fluid absorption. Domes appeared between days 4 and 8 of culture and persisted for about 1 week. Cell sheets showed a transepithelial resistance of ≈ 400 Ω·cm2 and a short-circuit current (Isc) of ≈ 5 μA·cm−2. The effects of transport inhibitors indicated that both active Na absorption and active Cl secretion were present. Isc was increased by isoproterenol, prostaglandins E2 and F2α, vasoactive intestinal peptide, dibutyryl cyclic AMP, leukotrienes C4 and D4, and bradykinin. These changes were probably due to stimulation of active Cl secretion.
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References
Abaza NA, Leighton J, Schultz SG (1974) Effects of ouabain on the function and structure of a cell line (MDCK) derived from canine kidney. I. Light microscopic observations of monolayer growth. In Vitro 10:172–183
Al-Bazzaz FJ (1981) Role of cyclic AMP in regulation of chloride secretion by canine tracheal mucosa. Am Rev Respir Dis 123:295–298
Al-Bazzaz FJ, Al-Awqati Q (1979) Interaction between sodium and chloride transport in canine tracheal mucosa. J Appl Physiol 46:111–119
Al-Bazzaz FJ, Cheng E (1979) Effect of catecholamines on ion transport in dog tracheal epithelium. J Appl Physiol 47:397–403
Al-Bazzaz FJ, Yadava VP, Westenfelder C (1981) Modification of Na and Cl transport in canine tracheal mucosa by prostaglandins. Am J Physiol 240:F101–F105
Bisbee CA, Machen TE, Bern HA (1979) Mouse mammary epithelial cells on floating collagen gels: transepithelial ion transport and effects of prolactin. Proc Natl Acad Sci USA 76:536–540
Boucher RC, Stutts MF, Gatzy JT (1981) Regional differences in bioelectric properties and ion flow in excised canine airways. J Appl Physiol 51:706–714
Burg MB, Green N, Sohraby S, Steele R, Handler J (1982) Differentiated function in cultured epithelia derived from thick ascending limbs. Am J Physiol 242:C229–C233
Coleman DL, Tuet IK, Widdicombe JH (1984) Electrical properties of dog tracheal epithelial cells grown in monolayer culture. Am J Physiol 246:C355–C359
Gordon RE (1985) Orthogonal arrays in normal and injured respiratory airway epithelium. J Cell Biol 100:648–651
Handler JS, Perkins FM, Johnson JP (1980) Studies of renal cell function using cell culture techniques. Am J Physiol 238:F1–F9
Johnson JP, Steele RE, Perkins FM, Wade JB, Preston AS, Green SW, Handler JS (1981) Epithelial organization and hormone sensitivity of toad urinary bladder cells in culture. Am J Physiol 241:F129–F138
Leikauf GD, Ueki IF, Nadel JA, Widdicombe JH (1985) Bradykinin stimulates chloride secretion and prostaglandin E2 release by canine tracheal epithelium. Am J Physiol 248:F48–F55
Leikauf GD, Ueki IF, Widdicombe JH, Nadel JA (1986) Alteration of chloride secretion across canine tracheal epithelium by lipoxygenase products of arachidonic acid. Am J Physiol 250:F47–F53
Lever JE (1979a) Regulation of dome formation in differentiated epithelial cell cultures. J Supramol Struct 12:259–272
Lever JE (1979b) Inducers of mammalian cell differentiation stimulate dome formation in a differentiated kidney epithelial cell line (MDCK). Proc Natl Acad Sci USA 76:1323–1327
Logsdon CD, Bisbee CA, Rutten MJ, Machen TE (1982) Fetal rabbit gastric epithelial cell cultured on floating collagen gels. In Vitro 18:233–242
Lowy RJ, Dawson DC, Ernst SA (1985) Primary culture of duck salt gland. II. Neurohumoral stimulation of active transport. Am J Physiol 249:C41–C47
Mason RJ, Williams MC, Widdicombe JH, Sanders M, Misfeldt DS, Berry L (1982) Transepithelial transport by pulmonary alveolar type II cells in primary culture. Proc Natl Acad Sci USA 79:6033–6037
Misfeldt DS, Hamamoto ST, Pitelka DR (1976) Transepithelial transport in cell culture. Proc Natl Acad Sci USA 73:1212–1216
Nadel JA, Widdicombe JH, Peatfield AC (1985) Regulation of airway secretions, ion transport and water movement. In: Fishman AP, Fisher AB (eds) Handbook of physiology, section 3: The respiratory system, volume I. Circulation and nonrespiratory functions. The American Physiological Society, Bethesda, pp 419–445
Nathanson I, Widdicombe JH, Barnes PJ (1983) Effect of vasoactive intestinal peptide on ion transport across dog tracheal epithelium. J Appl Physiol 55:1844–1848
Olver RE, Davis B, Marin MG, Nadel JA (1975) Active transport of Na+ and Cl− across the canine tracheal epithelium in vitro. Am Rev Resp Dis 112:811–815
Widdicombe JH, Welsh MJ (1980) Ion transport by dog tracheal epithelium. Fed Proc 39:3062–3066
Widdicombe JH, Basbaum CB, Highland E (1981) Ion contents and other properties of isolated cells from dog tracheal epithelium. Am J Physiol 241:C184–C192
Widdicombe JH, Nathanson IT, Highland E (1983) Effects of “loop” diuretics on ion transport by dog tracheal epithelium. Am J Physiol 245:C388–C396
Widdicombe JH, Coleman DL, Finkbeiner WE, Tuet IK (1985) Electrical properties of monolayers cultured from cells of human tracheal mucosa. J Appl Physiol 58:1729–1735
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Widdicombe, J.H., Coleman, D.L., Finkbeiner, W.E. et al. Primary cultures of the dog's tracheal epithelium: Fine structure, fluid, and electrolyte transport. Cell Tissue Res. 247, 95–103 (1987). https://doi.org/10.1007/BF00216551
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DOI: https://doi.org/10.1007/BF00216551