Summary
The central projections of primary afferents in the terminal ganglion of the crayfish can be seen when an axonal filling with nickel chloride with subsequent silver intensification was used for identification. We describe here the topological relationships of the projections to the landmark structures of the neuropil.
The terminal ganglion has five pairs of sensory nerves associated with the mechanosensory hairs and internal proprioceptors. The projection fields of the primary sensory neurons in the nerves Rl and R2 are almost entirely restricted to the ipsilateral half of the ganglion, whereas those of the nerves R3, R4 and R5 cross the midline to form three sensory commissures, A6SCI, A7SCI and A7SCII. The projection fields are segregated from each other, although all are restricted to the ventral neuropil which lies under the ventral intermediate tract (VIT). The intersegmental projections that ascend via the connective ipsilateral to their origins could be observed. This pattern of projection correlates well with the receptive fields exhibited by several mechanosensory interneurons on the body surface of the final segment.
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References
Altman JS, Tyrer NM (1977) The locust wing hinge stretch receptors. II. Variation, alternative pathways and “mistakes” in the central arborizations. J Comp Neurol 172:431–440
Bacon JP, Altmann JS (1977) A silver intensification method for cobalt-filled neurones in wholemount preparations. Brain Res 138:359–363
Burrows M, Siegler MVS (1985) Organization of receptive fields of spiking local interneurons in the locust with inputs from hair afferents. J Neurophysiol 53:1147–1157
Calabrese RL (1976a) Crayfish mechanoreceptive interneurons: I. The nature of ipsilateral excitatory input. J Comp Physiol 105:83–102
Calabrese RL (1976b) Crayfish mechanoreceptive interneurons: II. Bilateral interactions and inhibition. J Comp Physiol 105:103–114
Delcomyn F (1981) Nickel chloride for intracellular staining of neurons in insects. J Neurobiol 12:623–627
Gewecke M (1979) Central projection of antennal afferents for the flight motor in Locusta migratoria (Orthoptera, Acrididae). Entomol Gen 5:317–320
Hisada M, Takahata M, Nagayama T (1984a) Structure and output connection of local non-spiking interneurons in crayfish. Zool Sci 1:41–49
Hisada M, Takahata M, Nagayama T (1984b) Local non-spiking interneurons in the arthropod motor control systems. Zool Sci 1:681–700
Hustert R (1978) Segmental and interganglionic projections from primary fibers of insect mechanoreceptors. Cell Tissue Res 194:337–351
Kien J (1980) Morphology of locust neck muscle motoneurons and some of their inputs. J Comp Physiol 140:321–336
Kondoh Y, Hisada M (1986) Neuroanatomy of the terminal (sixth abdominal) ganglion of the crayfish, Procambarus clarkii Girard. Cell Tissue Res 243:273–288
Letourneau JG (1976a) Addition of sensory structures and associated neurons to the crayfish telson during development. J Comp Physiol 110:13–23
Letourneau JG (1976b) Somatotopic organization of afferent axons in peripheral nerves. J Comp Physiol 110:25–32
Murphy RK (1981) The structure and development of a somatotopic map in crickets: the cereal afferent projection. Dev Biol 88:236–246
Palka J, Lawrence PA, Hart HS (1979) Neural projection patterns from homeotic tissue of Drosophila studied in bithorax mutants and mosaics. Dev Biol 69:549–575
Pipa RL, Cook EF, Richards AG (1959) Studies on the hexapod nervous system. II. The histology of the thoracic ganglia of the adult cockroach, Periplaneta americana (L.). J Comp Neurol 113:410–433
Pflüger HJ, Bräunig P, Hustert R (1981) Distribution and specific central projections of mechanoreceptors in the thorax and proximal leg joints of locust. II. The external mechanoreceptors: hair plates and tactile hairs. Cell Tissue Res 216:79–96
Quicke DLJ, Brace RC (1979) Differential staining of cobalt and nickel-filled neurones using rubeanic acid. J Microsc 115:161–163
Reichert H, Plummer MR, Wine JJ (1983) Identified non-spiking local interneurons mediate nonrecurrent, lateral inhibition of crayfish mechanosensory interneurons. J Comp Physiol 151:261–276
Sandeman DC, Okajima A (1973) Statocyst induced eye movements in the crab Scylla serrata. III. The anatomical projections of sensory and motor neurons and the responses of the motor neurons. J Exp Biol 59:17–38
Strausfeld NJ (1976) Atlas of an insect brain. Springer, Berlin Heidelberg New York
Sigvardt KA, Hagiwara G, Wine JJ (1982) Mechanosensory integration in the crayfish abdominal nervous system: structural and physiological differences between interneurons with single and multiple spike initiating sites. J Comp Physiol 148:143–157
Takahata M, Hisada M (1982) Statocyst interneurons in the crayfish Procambarus clarkii Girard. II. Directional sensitivity and its mechanism. J Comp Physiol 149:301–306
Takahata M, Yoshino M, Hisada M (1985) Neuronal mechanisms underlying crayfish steering behavior as an equilibrium response. J Exp Biol 114:599–617
Tyrer NM, Bacon JP, Davies CA (1979) Sensory projections from wind-sensitive head hairs of the locust Schistocerca gregaria. Cell Tissue Res 203:79–92
Wiersma CAG, Hughes GM (1961) On the functional neuroanatomy of neuronal units in the abdominal cord of the crayfish, Procambarus clarkii (Girard). J Comp Neurol 116:209–228
Wiese K (1976) Mechanoreceptors for near-field water displacement in crayfish. J Neurophysiol 39:816–833
Wiese K, Calabrese RL, Kennedy D (1976) Integration of directional mechanosensory input by crayfish interneurons. J Neurophysiol 39:834–843
Wiese K, Schultz R (1982) Intrasegmental inhibition of the displacement-sensitive pathway in the crayfish (Procambarus clarkii). J Comp Physiol 147:447–454
Wilkens LA, Larimer JL (1972) The CNS photoreceptor of crayfish: morphology and synaptic activity. J Comp Physiol 80:389–407
Wilkens LA, Marzelli GA (1979) Central inhibition of an identified mechanosensory interneuron in the crayfish. J Neurobiol 10:247–254
Wine JJ (1984) The structural basis of an innate behavioral pattern. J Exp Biol 112:283–319
Wine JJ, Hagiwara G (1977) Crayfish escape behavior. I. The structure of efferent and afferent neurons involved in abdominal extension. J Comp Physiol 121:145–172
Wine JJ, Krasne FB (1982) The cellular organization of crayfish escape behavior. In: Sandeman DC, Atwood HL (eds) The Biology of Crustacea. Academic Press Inc. 4:241–292
Yoshino M, Kondoh Y, Hisada M (1983) Projection of Statocyst sensory neurons associated with crescent hairs in the crayfish Procambarus clarkii Girard. Cell Tissue Res 230:37–48
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Kondoh, Y., Hisada, M. The topological organization of primary afferents in the terminal ganglion of crayfish, Procambarus clarkii . Cell Tissue Res. 247, 17–24 (1987). https://doi.org/10.1007/BF00216542
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DOI: https://doi.org/10.1007/BF00216542