Summary
S-100 protein-immunoreactive cells were demonstrated by immunocytochemical procedures in the pancreatic islets of Langerhans in the monkey Macaca irus. By use of antibodies against human S-100 protein or bovine S-100 protein, these cells were observed in all islets in the head and tail portions of the pancreas. Immunostained cells were usually located in the center of the islets or sometimes found in a more widely distributed form, but they were never arranged in a regular concentric fashion. The number of immunoreactive cells varied from one islet to another but it was relatively limited making up only 0.75%–6.3% of all insular cells. With the use of the double-immunoenzymatic procedure for demonstration of the four main endocrine cell types (insulin-, glucagon-, somatostatin-and pancreatic polypeptide producing elements), it was possible to establish that S-100 protein-immunoreactive cells represent a distinct cell type. Antibodies against S-100 protein-stained neuroinsular complexes. The present findings speak in favor of a new cell type to be added to the large variety of S-100 protein-immunoreactive cells outside the central nervous system.
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Alumets J, Håkanson R, Sundler F (1983) Ontogeny of endocrine cells in porcine gut and pancreas: an immunocytochemical study. Gastroenterology 85:1359–1372
Agulleiro B, Ayala AG, Abad ME (1985) An immunocytochemical and ultrastructural study of the endocrine pancreas of Pseudemys scripta elegans (Chelonia). Gen Comp Endocrinol 60:95–103
Appel MC, Like AA (1982) Functional morphology of the islets of Langerhans. In: Brodoff BN, Beicher SJ (eds) Diabetes mellitus and obesity. Williams and Wilkins, Baltimore, pp 95–107
Baskin DG, Gorray KC, Fujimoto WY (1984) Immunocytochemical identification of cells containing insulin, glucagon, somatostatin-like and pancreatic polypeptide in the islets of Langerhans of the guinea pig pancreas with light and electron microscopy. Anat Rec 208:567–578
Buchan AM (1984) An immunocytochemical study of endocrine pancreas of snakes. Cell Tissue Res 235:657–661
Buchan AMJ, Lance V, Polak JM (1982) The endocrine pancreas of Alligator mississippiensis. An immunocytochemical investigation. Cell Tissue Res 224:117–128
Carrillo M, Zanuy S, Duve H, Thorpe A (1986) Identification of hormone-producing cells of the endocrine pancreas of the sea bass, Dicentrarchus labrax, by ultrastructural immunocytochemistry. Gen Comp Endocrinol 61:287–301
Cowap J (1985) The first appearance of endocrine cells in the splenic lobe of the embryonic chick pancreas. Gen Comp Endocrinol 60:131–137
DeLeo JA, Schweisthal MR (1984) Use of the peroxidase-antiperoxidase technique for differential staining of multiple cell types in the rat pancreatic islet. Anat Rec 209:417–422
Donev SR (1984) Ultrastructural evidence for the presence of a glial sheath investing the islets of Langerhans in the pancreas of mammals. Cell Tissue Res 237:343–348
El-Salhy M (1984) Immunocytochemical investigation of the gastro-entero-pancreatic (GEP) ncurohormonal peptides in the pancreas and gastrointestinal tract of the dogfish Squalus acanthias. Histochemistry 80:193–205
El-Salhy M, Grimelius L (1981) Histological and immunocytochemical studies of the endocrine pancreas of lizards. Histochemistry 72:237–247
El-Salhy M, Abu-Sinna G, Wilander E (1983) The endocrine pancreas of a squamate reptile, the desert lizard (Chalcides ocellatus). A histological and immunocytochemical investigation. Histochemistry 78:391–397
Erlandsen SL (1980) Types of pancreatic islet cells and their immunocytochemical identification. In: Fitzgerald PJ, Morrison AB (eds) Pancreas (Int Acad Pathol Monogr Pathol n∘ 21), Williams and Wilkins, Baltimore, pp 140–155
Fujita T (1959) Histological studies on the neuro-insular complex in the pancreas of some mammals. Z Zellforsch 50:94–109
Girod C, Trouillas J, Dubois MP (1985) Immunocytochemical localization of S-100 protein in stellate cells (folliculo-stellate cells) of the anterior lobe of the normal human pituitary. Cell Tissue Res 241:505–511
Grube D, Bohn R (1983) The microanatomy of human islets of Langerhans, with special reference to somatostatin (D-) cells. Arch Histol Jpn 46:327–353
Grube D, Eckert I, Speck PT, Wagner HJ (1983) Immunohistochemistry and microanatomy of the islets of Langerhans. In: Fujita T, Kanno T, Yanaihara N (eds) Brain-gut axis. Biomed Res [suppl] 4:25–36
Hara M, Tsutsumi Y (1986) Immunohistochemical studies of endocrine cells in heterotopic pancreas. Virchows Arch [A] 408:385–394
Kaung HC (1985) Electron microscopic immunocytochemical localization of glucagon and pancreatic polypeptide in rat pancreas: characterization of a population of islet cells containing both peptides. Anat Rec 212:292–300
Kaung HC, Elde RP (1980) Distribution and morphometric quantitation of pancreatic endocrine cell types in the frog, Rana pipiens. Anat Rec 196:173–181
Klein C, Van Noorden S (1978) Use of immunocytochemical staining of somatostatin for correlative light and electron microscopic investigation of D cells in the pancreatic islet of Xiphophorus helleri H (Teleostei). Cell Tissue Res 194:399–404
Klein C, Van Noorden S (1980) Pancreatic polypeptide (PP)-and glucagon cells in the pancreatic islet of Xiphophorus helleri H (Teleostei). Correlative immunohistochemistry and electron microscopy. Cell Tissue Res 205:187–198
Klöppel G, Bommer G, Commandeur G, Heitz P (1978) The endocrine pancreas in chronic pancreatitis. Immunocytochemical and ultrastructural studies. Virchows Arch [A] 377:157–174
Kramer B, Andrew A (1981) Further investigation into the source of pancreatic endocrine cell types. Gen Comp Endocrinol 44:279–287
L'Hermite A, Ferrand E, Dubois MP, Andersen AC (1985) Detection of endocrine cells by immunofluorescence method in the gastroenteropancreatic system of the adult eel, glass-eel, and leptocephalic larva (Anguilla anguilla L.). Gen Comp Endocrinol 58:347–359
McNeill DL, Brinn JE, Fletcher DJ (1984) An immunocytochemical study of the pancreatic islet system of the channel catfish. Anat Rec 209:381–384
Mikami S, Taniguchi K, Ishikawa T (1985) Immunocytochemical localization of the pancreatic islet cells in the Japanese quail, Coturnix coturnix japonica. Jpn J Vet Sci 47:357–369
Munger BL (1981) Morphological characterization of islet cell diversity. In: Cooperstein SJ, Watkins D (eds) The islets of Langerhans. Academic Press, New York, pp 3–34
Nakajima T, Kameya T, Watanabe S, Hirota T, Shimosato Y (1984) S-100 protein distribution in normal and neoplastic tissues. In: DeEellis RA (ed) Advances in immunohistochemistry. Masson Publ USA, New York, pp 141–158
Orci L (1984) Patterns of cellular and subcellular organization in the endocrine pancreas. J Endocrinol 102:3–11
Orci L (1985) The insulin factory: a tour of the plant surrounding and a visit to the assembly line. Diabetologia 28:528–546
Pelletier G (1977) Identification of four cell types in the human endocrine pancreas by immunoelectron microscopy. Diabetes 26:749–756
Reddy SN, Bibby NJ, Elliott RB (1985) Cellular distribution of insulin, glucagon, pancreatic polypeptide hormone and somatostatin in the fetal and adult pancreas of the guinea pig: a comparative immunohistochemical study. Eur J Cell Biol 38:301–305
Rhoten WB (1984) Immunocytochemical localization of four hormones in the pancreas of the garter snake, Thamnophis sirtalis. Anat Rec 208:233–242
Rhoten WB, Hall CE (1981) Four hormones in the pancreas of the lizard, Anolis carolinensis. Anat Rec 199:89–97
Rhoten WB, Hall CE (1982) An immunocytochemical study of the cytogenesis of pancreatic endocrine cells in the lizard, Anolis carolinensis. Am J Anat 163:181–193
Rhoten WB, Smith PH (1978) Localization of four polypeptide hormones in the saurian pancreas. Am J Anat 151:595–602
Rombout JHWM, Taverne-Thiele JJ (1982) An immunocytochemical and electron-microscopical study of endocrine cells in the gut and pancreas of a stomachless teleost fish, Barbus conchonius (Cyprinidae). Cell Tissue Res 227:577–593
Sekine Y, Yui R (1981) Immunohistochemical study of the pancreatic endocrine cells of the ray, Dasyatis akajei. Arch Histol Jpn 44:95–101
Smith H (1975) Structural modification of Schwann cells in the pancreatic islets of the dog. Am J Anat 144:513–517
Stefan Y, Falkmer S (1980) Identification of four endocrine cell types in the pancreas of Cottus scorpius (Teleostei) by immunofluorescence and electron microscopy. Gen Comp Endocrinol 42:171–178
Stefan Y, Orci L, Malaisse-Lagae F, Perrelet A, Patel Y, Unger RH (1982) Quantitation of endocrine cell content in the pancreas of nondiabetic and diabetic humans. Diabetes 31:694–700
Stefan Y, Grasso S, Perrelet A, Orci L (1983) A quantitative immunofluorescence study of the endocrine cell population in the developing human pancreas. Diabetes 32:293–301
Takahashi K, Isobe T, Ohtsuki Y, Akagi T, Sonobe H, Okuyama T (1984) Immunohistochemical study of the distribution of a and β of S-100 protein in human noeplasms and normal tissues. Virchows Arch [Cell Pathol] 45:385–396
Tomita T, Pollock HG (1981) Four pancreatic endocrine cells in the bullfrog (Rana catesbeiana). Gen Comp Endocrinol 45:355–363
Tramu G, Pilliez A, Léonardelli J (1978) An efficient method of antibody elution for the successive or simultaneous localization of two antigens by immunocytochemistry. J. Histochem Cytochem 26:322–324
Van Noorden S, Falkmer S (1980) Gut-islet endocrinology. Some evolutionary aspects. Invest Cell Pathol 3:21–35
Vanstapel MJ, Gatter KC, DeWolf-Peeters C, Mason DY, Desmet VD (1986) New sites of human S-100 immunoreactivity detected with monoclonal antibodies. Am J Clin Pathol 85:160–168
Wagner GF, McKeown BA (1981) Immunocytochemical localization of hormone-producing cells within the pancreatic islets of the rainbow trout (Salmo gairdneri). Cell Tissue Res 221:181–192
Yoshida K, Iwanaga T, Fujita T (1983) Gastro-entero-pancreatic (GEP) endocrine system of the flatfish, Paralychtys olivaceus: an immunocytochemical study. Arch Histol Jpn 46:259–266
Yoshinari M, Daikoku S (1982) Ontogenic appearance of immunoreactive endocrine cells in rat pancreatic islets. Anat Embryol 165:63–70
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Girod, C., Durand, N. & Raccurt, M. Immunostaining of a cell type in the islets of Langerhans of the monkey Macaca irus by antibodies against S-100 protein. Cell Tissue Res. 247, 11–16 (1987). https://doi.org/10.1007/BF00216541
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DOI: https://doi.org/10.1007/BF00216541