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Demonstration of microtubules in the terminal web of mature absorptive cells from the small intestine of the rat

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Summary

The terminal web (TW) region of mature absorptive cells in the small intestine of the rat contains an elaborate cytoskeleton which supports the apical microvillus membrane. In studies regarding the structural organization of the cytoskeleton and associated proteins in the small intestine, microtubules have not been mentioned as components of the TW. By transmission electron microscopy of conventional resin-embedded sections of rat small intestine, we observe many microtubule profiles in the TW of mature absorptive cells. These microtubules are found in various orientations, although most course parallel to the long axis of the cell, and many microtubule profiles are seen in close association with smooth-surfaced vesicles.

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References

  • Blok J, Ginsel LA, Mulder-Stapel AA, Onderwater JJM, Daems WTh (1981) The effect of colchicine on the intracellular transport of 3H-fucose-labelled glycoproteins in the absorptive cells of cultured human small intestinal tissue. Cell Tissue Res 215:1–12

    Google Scholar 

  • Buschmann RJ (1983) Morphometry of the small intestinal enterocytes of the fasted rat and the effects of colchicine. Cell Tissue Res 231:289–299

    Google Scholar 

  • Danielsen EM, Cowell GM, Poulsen SS (1983) Biosynthesis of intestinal microvillar proteins: Role of the Golgi complex and micro tubules. Biochem J 216:37–42

    Google Scholar 

  • Ellinger A, Pavelka M, Gangl A (1983) Effect of colchicine on rat intestinal absorptive cells II. Distribution of label after incorporation of 3H-fucose into plasma membrane glycoproteins. J Ultrast Res 85:260–271

    Google Scholar 

  • Ginsel LA, Debets WF, Daems WTh (1975) The effect of colchicine on the distribution of apical vesicles and tubules in absorptive cells of cultured human small intestine. In: Borgers M, de Brabander M (eds) Microtubules and microtubule inhibitors. North Holland Publishing Company, Amsterdam, pp 187–198

    Google Scholar 

  • Gorbsky G, Borisy GG (1985) Microtubule distribution in cultured cells and intact tissues: Improved immunolabeling resolution through the use of reversible embedment cytochemistry. Proc Natl Acad Sci USA 82:6889–6893

    Google Scholar 

  • Hirokawa N, Heuser JE (1981) Quick-freeze, deep-etch visualization of the cytoskeleton beneath surface differentiations of intestinal epithelial cells. J Cell Biol 91:399–409

    Google Scholar 

  • Hirokawa N, Tilney LG, Fujiwara K, Heuser JE (1982) Organization of actin, myosin, and intermediate filaments in the brush border of intestinal epithelial cells. J Cell Biol 94:425–443

    Google Scholar 

  • Hirokawa N, Cheney RE, Willard M (1983) Location of a protein of the fodrin-spectrin — TW 260/240 family in the mouse intestinal brush border. Cell 32:953–965

    Google Scholar 

  • Keller TCS, Mooseker MS (1982) Ca++-calmodulin-dependent phosphorylation of myosin and its role in brush border contraction in vitro. J Cell Biol 95:943–959

    Google Scholar 

  • Mooseker MS, Keller TCS, Hirokawa N (1983) Regulation of contractility and cytoskeletal structure in the brush border. In: Brush border membranes. CIBA Foundation Symposium 95, Pitman, London pp 195–215

    Google Scholar 

  • Mooseker MS, Bonder EM, Conzelman KA, Fishkind DJ, Howe CL, Keller TGS (1984) Brush border cytoskeleton and integration of cellular functions. J Cell Biol 99:104s-112s

    Google Scholar 

  • Quaroni A, Kirsch K, Weiser MM (1979) Synthesis of membrane glycoproteins in rat small intestinal villus cells. Effect of colchicine on the redistribution of L-(1,5,6-3H)-fucose-labelled membrane glycoproteins among Golgi, basal lateral and microvillus membranes. Biochem J 182:213–222

    Google Scholar 

  • Reaven EP, Reaven GM (1977) Distribution and content of microtubules in relation to the transport of lipid: An ultrastructural quantitative study of the absorptive cell of the small intestine. J Cell Biol 75:559–572

    Google Scholar 

  • Sandoz D, Laine MC (1985) Distribution of microtubules within the intestinal terminal web as revealed by quick-freezing and cryosubstitution. Eur J Cell Biol 39:481–484

    Google Scholar 

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Authors and Affiliations

  1. Division of Gastroenterology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, and the Harvard Digestive Disease Center, Boston, Massachusetts, USA

    Susan J. Hagen Ph.D., Carol H. Allan & Jerry S. Trier

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  1. Susan J. Hagen Ph.D.
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  2. Carol H. Allan
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  3. Jerry S. Trier
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Hagen, S.J., Allan, C.H. & Trier, J.S. Demonstration of microtubules in the terminal web of mature absorptive cells from the small intestine of the rat. Cell Tissue Res. 248, 709–711 (1987). https://doi.org/10.1007/BF00216503

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  • DOI: https://doi.org/10.1007/BF00216503

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