Summary
The neurons in the substantia gelatinosa of the spinal cord of the rat and Japanese monkey were investigated electron microscopically and ultracytochemically. The confronting cisternae observed in the cytoplasm of the majority of gelatinosal neurons in both species were composed of closely apposed parallel cisternae with electron-dense flocculent material, and a continuity with the cisternae of the rough endoplasmic reticulum was often observed. Ultracytochemically, the p-nitrophenylphosphatase (p-NPPase) activity was present in the confronting cisternae, but thiamine monophosphatase (TMPase) and thiamine pyrophosphatase (TPPase) activities were absent. These results indicate that the confronting cisternae are a variant of the rough endoplasmic reticulum.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brawer JR (1971) The role of the arcuate nucleus in the brain-pituitary-gonad axis. J Comp Neurol 143:411–446
Cervero F, Iggo A (1980) The substantia gelatinosa of the spinal cord. Brain 103:717–772
Ghadially FN (1982) Endoplasmic reticulum. In: Ghadially FN (ed) Ultrastructural pathology of the cell and matrix. Butterworths, London Boston Sydney Wellington Durban Toronto, pp 315–422
Goldfischer S, Essner E, Novikoff AB (1964) The localization of phosphatase activities at the level of ultrastructure. J Histochem Cytochem 12:72–95
Inomata K, Ogawa K (1979) Ultracytochemical localization of thiamine monophosphatase activity in the substantia gelatinosa of the rat spinal cord. Acta Histochem Cytochem 12:337–343
Inomata K, Fujimoto K, Mayahara H, Ogawa K (1980) Ultrastructural localization of potassium-dependent, ouabain-sensitive p-nitrophenylphosphatase (K-NPPase) activity in the substantia gelatinosa of the rat spinal cord. J Electron Microsc (Tokyo) 29:300 (Abstract)
King JC, Williams TH, Gerall AA (1974) Transformations of hypothalamic arcuate neurons. I. Changes associated with the stages of the estrous cycle. Cell Tissue Res 153:497–515
Mayahara H, Fujimoto K, Ando T, Ogawa K (1980) A new one-step method for the cytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenyl-phosphatase activity. Histochemistry 67:125–138
Melzack R, Wall PD (1965) Pain mechanism: a new theory. Science 150:971–979
Nathaniel EJH, Nathaniel DP (1966) Fine structure of the neurons of the posterior horn in the rat spinal cord. Anat Rec 155:629–642
Ogawa K, Sakai M, Inomata K (1982) Recent findings on ultracytochemistry of thiamine phosphatases. Ann NY Acad Sci 378:188–214
Osinchak J (1964) Electron microscopic localization of acid phosphatase and thiamine pyrophosphatase activity in hypothalamic neurosecretory cells of the rat. J Cell Biol 21:35–47
Ramón y Cajal S (1980) Nuevas observaciones sobre la estructura de la médula espinal de los mamiferos. In: Laboratorio Anatómico de la Faculted de Medicina. Barcelona, Casa Provincial de Caridad
Réthelyi M, Szentágothai J (1969) The large synaptic complexes of the substantia gelatinosa. Exp Brain Res 7:258–274
Saito T, Ogawa K (1966) Electron cytochemical examination of the thiamine pyrophosphatase activity in the intestine of the rat. Arch Histol Jpn 27:473–484
Tice LW, Barnett RJ (1963) The fine structural localization of some testicular phosphatases. Anat Rec 147:43–63
Ueno S, Mayahara H, Tsukahara I, Ogawa K (1981) Ultracytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase activity in guinea-pig retina. II Neurons and Müller cells. Acta Histochem Cytochem 14:186–206
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Inomata, K., Okada, T., Seguchi, H. et al. The confronting cisternae in neurons in the substantia gelatinosa of the spinal cord of rats and Japanese monkeys. Cell Tissue Res. 232, 487–492 (1983). https://doi.org/10.1007/BF00216423
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00216423