Summary
By use of the peroxidase-antiperoxidase-complex (PAP) immunohistological method, the preoptico-infundibular LHRH-tract was studied in adult female rats in which frontal hypothalamic deafferentation was performed at the third or tenth postnatal day. In the former group, this LHRH-tract appeared to be similar to that of the intact controls; the animals showed regular vaginal cycles and ova were present in their oviducts. In the latter group, however, marked reduction in the number of the LHRH-nerve fibers was observed behind the sites of the deafferentation in the mediobasal hypothalamus (MBH), whereas LHRH-immunoreactive perikarya and nerve fibers containing the immunoreactive material were seen rostral to the plane of severance. In these animals reduction of LHRH-fibers in the MBH was accompanied by an anovulatory syndrome characterized by constant vaginal cornification and polyfollicular ovaries. Comparing the glial scar formation induced by the cut, significant differences were detected between the two experimental groups. In the animals deafferented on the 3rd day of life, reduction of nerve cells was seen along the cut, but LHRH-fibers crossing the thin glial scar were detectable in large numbers. On the other hand, in the animals deafferented on the 10th postnatal day, extensive glial scar tissue appeared to interrupt the LHRH-fibers rostral to the cut.
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References
Adams JH, Daniel PM, Prichard MML (1969) Degeneration and regeneration of the hypothalamic nerve fibers in the neurohypophysis after pituitary stalk section in the ferret. J Comp Neurol 135:121–124
Barry J, Dubois MP, Poulain P (1973) LRF producing cells in the mammalian hypothalamus. Z Zellforsch 146:351–366
Björklund A, Steveni U (1979) Regeneration of monoaminergic and cholinergic neurons in the mammalian central nervous system. Physiol Rev 55:62–100
Dellmann HD (1973) Degeneration and regeneration of neurosecretory system. Int Rev Cytol 36:215–315
Flerkó B, Sétáló G, Vigh S, Merchenthaler I (1980) Recent immunohistological findings on the LHRH neuron system of the rat. Mat Med Pol 12:119–123
Halász B, Gorski RA (1967) Gonadotrophic hormone secretion in female rats after partial or total interruption of neural afferents to the medial basal hypothalamus. Endocrinology 80:608–622
Harding J, Graziadei PPC, Graziadei GAM, Margolis FL (1977) Denervation in the primary olfactory pathway of mice. IV. Biochemical and morphological evidence for neuronal replacement following nerve section. Brain Res 132:11–28
Hayashi S (1974) Failure of intrahypothalamic implants of antiestrogen, MER-25, to inhibit androgen sterilization in female rats. Endocrinol Jpn 21:453–457
Hayashi S, Mizukami S (1979a) Effects of transection of anterior connections to the mediobasal hypothalamus on the onset of sexual cycles in neonatal female rats. Exp Neurol 63:211–219
Hayashi S, Mizukami S (1979b) Spontaneous ovulation in rats after interruption of anterior connections to the mediobasal hypothalamus at two days of age. Neuroendocrinology 28:145–150
Ibata Y, Watanabe K, Kinoshita H, Kubo S, Sano Y, Sin S, Hashimura E, Imagawa K (1979) The location of LHRH neurons in the rat hypothalamus and their pathways to the median eminence. Cell Tissue Res 198:381–395
Jennes L, Stumpf WE (1980) LHRH-system in the brain of the golden hamster. Cell Tissue Res 209:239–256
Kalra SP (1976) Tissue levels of luteinizing hormone-releasing hormone in the preoptic area and the hypothalamus, and serum concentrations of gonadotropins following anterior hypothalamic deafferentation and estrogen treatment of the female rat. Endocrinology 99:101–107
Kozlowski GP, Chu L, Hostetter G, Kerdelhué B (1980) Cellular characteristics of immunolabeled luteinizing hormone releasing hormone (LHRH) neurons. Peptides 1:37–46
Köves K, Halász B (1970) Location of the neural structures triggering ovulation in the rat. Neuroendocrinology 6:180–193
Krieg WJS (1932) The hypothalamus of the albino rat. J Comp Neurol 55:19–89
Krisch B (1978) The distribution of LHRH in the hypothalamus of the thirsting rat. Cell Tissue Res 186:135–148
Merchenthaler I, Kovács G, Lovász G, Sétáló G (1980a) The preoptico-infundibular LHRH tract of the rat. Brain Res 198:63–74
Merchenthaler I, Lengvári I, Horváth J, Sétáló G (1980b) Immunohistochemical study of the LHRH-synthesizing neuron system of aged female rats. Cell Tissue Res 209:499–503
Sechzer JA (1974) Axonal regeneration or generation after corpus callosum section in the neonatal rat. Exp Neurol 45:186–188
Sétáló G, Vigh S, Schally AV, Arimura A, Flerkó B (1976) Immunohistological study of the origin of LHRH-containing nerve fibers of the rat hypothalamus. Brain Res 103:597–602
Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. Preparation and properties of solube antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Vigh S, Sétáló Gy, Schally AV, Arimura A, Flerkp B (1978) LHRH-containing nerve fibers in the brain of rats treated with sulpiride or reserpine. Brain Res 152:401–405
Weiner RI, Pattou E, Kerdelhué B, Kordon C (1975) Differential effects of hypothalamic deafferentations upon luteinizing hormone releasing hormone in the median eminence and organum vasculosum of the lamina terminalis. Endocrinology 97:1597–1600
Zamboni L, De Martino L (1967) Buffered picric acid-formaldehyde: a new rapid fixative for electronmicroscopy. J Cell Biol 35:148A
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Liposits, Z., Nagy, L. & Sétáló, G. Frontal deafferentation of the mediobasal hypothalamus in the neonatal rat and its effects on the preoptico-infundibular LHRH-tract. Cell Tissue Res. 225, 179–187 (1982). https://doi.org/10.1007/BF00216227
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DOI: https://doi.org/10.1007/BF00216227