Summary
Location and distribution of nerve fibers immunoreactive to substance P were studied in the mouse olfactory mucosa. A moderately dense plexus of fibers is present at the interface of the olfactory epithelium and the connective tissue of the lamina propria. In addition, many immunoreactive nerve fibers are noted in close association with Bowman's glands and blood vessels in the lamina propria. However, such fibers were not observed in olfactory epithelium proper nor in the fila olfactoria. Substance-P-immunoreactivity is almost totally abolished by treatment of animals with capsaicin, an agent known to deplete substance P from primary sensory neurons. It is suggested that the substance-P-immunoreactive fibers are of sensory origin, with their perikarya most likely located in the trigeminal ganglia. Functionally, they might influence local blood flow and/or the secretion of Bowman's glands.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Costa M, Buffa R, Furness JB, Solcia E (1980) Immunohistochemical localization of polypeptides in peripheral autonomic nerves using whole mount preparations. Histochemistry 65:157–165
Cuello AC, McQueen DS (1980) Substance P: A carotid body peptide. Neurosci Lett 17:215–219
Cuello AC, Del Fiacco M, Paxinos G (1978) The central and peripheral ends of the substance P-containing sensory neurones in the rat trigeminal system. Brain Res 152:499–509
Eskay RL, Furness JB, Long RT (1981) Substance P activity in the bullfrog retina: localization and identification in several vertebrate species. Science 212:1049–1051
Furness JB, Papka RE, Della NG, Costa M, Eskay RL (1982) Substance P-like immunoreactivity in nerves associated with the vascular system of guinea pigs. Neuroscience 7:447–459
Gamse R, Holzer P, Lembeck F (1980) Decrease of substance P in primary afferent neurones and impairment of neurogenic plasma extravasation by capsaicin. Brit J Pharmacol 68:207–213
Hökfelt T, Kellerth JO, Nilsson G, Pernow B (1975) Substance P: localization in the central nervous system and in some primary sensory neurons. Science 190:889–890
Hökfelt T, Johansson O, Kellerth JO, Ljungdahl Å, Nilsson G, Nygårds A, Pernow B (1977) Immunohistochemical distribution of substance P. In: Von Euler US, Pernow B (eds) Substance P. Raven Press, New York, p 117
Holzer P, Gamse R, Lembeck F (1980) Distribution of substance P in the rat gastrointestinal tract — lack of effect of capsaicin pretreatment. Eur J Pharmacol 61:303–307
Kidd A, Garrett JR (1979) New observations on the innervation of striated ducts in submandibular glands of cats, including possible peptidergic nerves. Cell Tissue Res 200:205–214
Leeman SE, Hammerschlag R (1967) Stimulation of salivary secreton by a factor extracted from hypothalamic tissue. Endocrinology 81:803–810
Lembeck F (1953) Zur Frage der zentralen Übertragung afferenter Impulse. III. Mitteilung. Das Vorkommen und die Bedeutung der Substanz P in die dorsalen Wurzeln des Rückenmarks. Naunyn-Schmiedebergs Arch Pharmacol 219:197–213
Lembeck F, Holzer P (1979) Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. Naunyn-Schmiedebergs Arch Pharmacol 310:175–183
Lembeck F, Zetler G (1962) Substance P: a polypeptide of possible physiological significance, especially within the nervous system. Int Rev Neurobiol 4:159–215
Liang T, Cascieri MA (1981) Substance P receptor on parotid cell membranes. J Neurosci 1:1133–1141
Lundberg JM, Hökfelt T, Änggard A, Pernow B, Emson P (1979) Immunohistochemical evidence for substance P immunoreactive nerve fibers in the taste buds of the cat. Acta Physiol Scand 107:389–391
Matulionis DH (1974) Ultrastructure of olfactory epithelia in mice after smoke exposure. Ann Oto Rhino Layrngo 83:192–201
Matulionis DH (1975) Ultrastructural study of mouse olfactory epithelium following destruction by ZnSO4 and its subsequent regeneration. Am J Anat 142:67–90
Miller A, Costa M, Furness JB, Chubb IW (1981) Substance P immunoreactive sensory nerves supply the rat iris and cornea. Neurosci Lett 23:243–249
Otsuka M, Konishi S (1975) Substance P and excitatory transmitter of primary sensory neurons. Cold Spring Harbor Symp Quant Biol 40:135–143
Otsuka M, Konishi S (1977) Electrophysiological and neurochemical evidence for substance P as a transmitter of primary sensory neurons. In: von Euler US, Pernow B (eds) Substance P. Raven Press, New York, p 207
Reinecke M, Weihe E, Forssmann WG (1980) Substance P-immunoreactive nerve fibers in the heart. Neurosci Lett 20:265–269
Robinson SE, Schwartz JP, Costa E (1980) Substance P in the superior cervical ganglion and submaxillary gland of the rat. Brain Res 182:11–17
Takahashi T, Otsuka M (1975) Regional distribution of substance P in the spinal cord and nerve roots of the cat and the effect of dorsal root section. Brain Res 87:1–11
Weihe E, Reinecke M, Opherk D, Forssmann WG (1981) Peptidergic innervation (substance P) in the human heart. J Molec Cell Cardiol 13:331–333
Wharton J, Polak JM, McGregor GP, Bishop AE, Bloom SR (1981) The distribution of substance P-like immunoreactive nerves in the guinea-pig heart. Neuroscience 6:2193–2204
Author information
Authors and Affiliations
Additional information
Supported in part by PHS BRSG 2507RR05374-20, by Grant 1R01H122226 from NHBLI and Tobacco and Health Research Institute Grant 124-05-7H100-4A007
Rights and permissions
About this article
Cite this article
Papka, R.E., Matulionis, D.H. Association of substance-P-immunoreactive nerves with the murine olfactory mucosa. Cell Tissue Res. 230, 517–525 (1983). https://doi.org/10.1007/BF00216198
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00216198