Abstract
The functional and ultrastructural stability of bovine platelets, stored in homologous plasma at 22 °C for periods up to 96 h, were examined. In only approximately 22% of the platelets stored for the full period was there any evidence of moderate ultrastructural alterations compared to the freshly prepared samples. The most obvious morphological changes in the stored platelets included an increase in the number of dense granules in contact with the platelet membrane and an increased heterogeneity in the appearance of the alpha granules. The microtubules of the circumferential band remained intact and there was minimal evidence of activation of the bovine platelets due to storage. The functional response to ADP, platelet activating factor (PAF) or collagen was maintained for the first 48 h of storage. The aggregation response to ADP was 81.3% of the initial value after 96 h of storage whereas the response to PAF was 86.2% and 54.7% of the initial value at 72 and 96 h respectively. Although the collagen aggregation response also declined to 54.8% of the initial value by 96 h, the release of thromboxane in response to collagen stimulation was similar in the stored and fresh samples.
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References
Adams GA, Rock G (1988) Storage of human platelet concentrates in an artificial medium without dextrose. Transfusion 28:217–220
Adams GA, Swenson SD, Rock G (1986) Survival and recovery of human platelets stored for 5 days in a non-plasma medium. Blood 67:672–675
Bentfield ME, Bainton DF (1982) Identification of primary lysosomes in human megakaryocytes and platelets. J Clin Invest 56:1635–1649
Bode AP, Miller DT (1986) Analysis of platelet factor 3 in platelet concentrates stored for transfusion. Vox Sang 51:299–305
Bondy GS, Gentry PA (1989) Characterization of the normal bovine platelet aggregation response. Comp Biochem Physiol 92:67–72
Bondy GS, Gentry PA, Basrur PK (1988) Structure-function relationship of the action of T-2 toxin on bovine platelets. Fundam Appl Toxicol 12:109–116
DiMiano G, Silver MJ, Murphy S (1982) Stored human platelets retain full aggregation potential in response to pairs of aggregating agents. Blood 59:563–568
Edenbrandt CM, Murphy S (1990) Adenine and guanine metabolism during platelet storage at 22 °C. Blood 76:1884–1892
Feinberg H, Sarin MM, Batka EA et al. (1988) Platelet storage: changes in cytosolic Ca2+ actin polymerization and shape. Blood 72:766–769
Fijnheer R, Homburg HE, Hooibrink B et al. (1991) Loss of thrombin-induced calcium mobilization in a subpopulation of platelets during storage. Thromb Haemost 66:350–354
Fratantoni JC, Poindexter BJ, Bonner RF (1984) Quantitative assortment of platelet morphology by light scattering: a potential method for the evaluation of platelets for transfusion. J Lab Clin Med 103:620–631
Gentry PA, Tremblay RM, Ross ML (1989) Failure of aspirin to impair bovine platelet function. Am J Vet Res 50:919–922
Gottschall JL, Rzad L, Aster RH (1986) Studies on the minimum temperature which human platelets can be stored with full maintenance of viability. Transfusion 26:460–462
Grouse LH, Rao GHR, Weiss DJ et al. (1990) Surface-activated bovine platelets do not spread, they unfold. Am J Pathol 136:399–408
Hervig T, Volundardottir T, Bakken AM et al. (1990) Thrombin-induced serotonin release as an in vitro indicator of the functional integrity of stored platelets. Clin Chem 36:28–31
Holme S, Vaidya K, Murphy S (1978) Platelet storage at 22 °C; effect of type of agitation on morphology, viability and function in vitro. Blood 52:425–435
Holmsen H (1985) Platelet metabolism and activation. Semin Hematol 22:219–240
Kakaiya RM, Cable RG (1985) The aggregation defect of platelets stored at room temperature in new formulation plastic containers. Vox Sang 49:368–369
Kilksow H, Holmes S, Murphy S (1984) Platelet metabolism during storage of platelet concentrates at 22 °C. Blood 64:406–414
Kunicki TJ, Tuccelli M, Becker GA et al. (1975) A study of variables affecting the quality of platelets stored at room temperature. Transfusion 15:414–421
Menard M, Meyers KM (1988) Storage pool deficiency in cattle with the Chediak-Higashi syndrome results from an absence of dense granule precursors in their megakaryocytes. Blood 72:1726–1734
Menard M, Meyers KM, Prieur DJ (1990) Demonstration of secondary lysosomes in bovine megakaryocytes and platelets using acid phosphatase cytochemistry with cerium as a trapping agent. Thromb Haemost 63:127–132
Meyers KM (1986) Species differences. In: Holmsen H (ed) Platelet responses and metabolism. CRC Press, Boca Raton, Florida, pp 209–234
Meyers RM, Katz JB, Clemens RM et al. (1980) An evaluation of the arachidonate pathway of platelets from companion and food-producing animals, mink, and man. Thromb Res 20:13–24
Meyers KM, Hopkins G, Holmsen H et al. (1982) Ultrastructure of resting and activated storage pool deficient platelets from animals with the Chediak-Higashi syndrome. Am J Pathol 106:364–377
Murphy S (1985) Platelet storage for transfusion. Semin Hematol 22:165–177
Murphy S, Gardner FH (1971) Platelet storage at 22 °C; metabolic, morphologic, and functional studies. J Clin Invest 50:370–377
Murphy S, Kahn RA, Holme S et al. (1982) Improved storage of platelets for transfusion in a new container. Blood 60:194–200
Pietersz RNI, Loos JA, Reesink HW (1987) Survival in vivo of platelets stored for 48 hrs in the buffy coat at 4 °C compared to platelet rich plasma stored at 25 °C. Blut 54:201–206
Rao AK, Niewiarowski S, Murphy S (1981) Acquired granular pool defect in stored platelets. Blood 57:203–208
Rock G, Senack E, Tittley P (1989) 5-day storage of platelets collected on a blood cell separator. Transfusion 29:626–628
Snyder EL, Bookbinder M, Kakaiya R et al. (1983) 5-day storage of platelet concentrates in CLX containers: effect of type of agitation. Vox Sang 45:432–437
Solberg C, Holme S, Little C (1986) Morphological changes associated with pH changes during storage of platelet concentrates in first-generation 3-day container. Vox Sang 50:71–77
Treachard DM (1988) In vitro platelet sensitivity to collagen-induced aggregation and in vitro platelet determination rate during storage, are both inversely proportional to the in vivo blood platelet concentration in normal individuals: preliminary documentation of these theoretically predictable relationships. Thromb Res 51:425–437
Washitani V, Irita Y, Yamamoto K et al. (1988) Prevention of acquired defect in platelet function during blood processing. Transfusion 28:571–575
Watts SE, Tunbridge, LJ, Smith K et al. (1986) Storage of platelets for tests of platelet function: effects of temperature on platelet aggregation, platelet morphology and liberation of β-thromboglobulin. Thromb Res 44:365–376
White JG (1968) Fine structural alterations induced in platelets by adenosine diphosphate. Blood 31:604–622
White JG (1981) Ultrastructural lesions of stored platelets. Vox Sang 40 (Suppl 1):62–68
Zucker-Franklin D, Benson KA, Meyers KM (1985) Absence of a surface-connected canalicular system in bovine platelets. Blood 65:241–244
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Gentry, P.A., De Jong, C.G. & Basrur, P.K. Stability of bovine platelets stored at ambient temperature. Comparative Haematology International 2, 213–219 (1992). https://doi.org/10.1007/BF00216097
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DOI: https://doi.org/10.1007/BF00216097