Summary
The ultrastructure of macrophages and reticular cells of regional lymph nodes of the rat after administration of large doses of cortisone acetate, estrone, progesterone, and cholesterol in aqueous suspensions was investigated. A large number of vacuoles, most of which were surrounded by unit membrane, and lipid droplets not surrounded by unit membrane were observed in the cytoplasm of both macrophages and reticular cells. They were not seen in these cells of control animals and in experimental animals that had received smaller doses of these steroid hormones. After cholesterol injection, many lipid droplets were observed in the cytoplasm of macrophages. These observations suggest that steroids injected in suspension accumulate in macrophages and reticular cells of the regional lymph nodes. Electron-dense material was often present in vacuoles of macrophages but not in those of reticular cells.
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References
Arthur MD Jr (1979) The antiinflammatory effects of glucocorticosteroids. A brief review of the literature. Inflammation 3:329–343
Buchmuller Y, Mauel J (1977) Activation of macrophages by supernatants of Con A-stimulated lymphocytes. Experientia 33:814
Clark LS (1962) The reticulum of lymph nodes in mice studied with the electron microscope. Am J Anat 110:217–257
Duve C, Barsy T, Poole B, Trouet A, Tulkens P, Van Hoof F (1974) Lysosomotropic agents. Biochem Pharmacology 23:2495–2531
Farr GA, De Bruyn PPH (1975) Macrophage-lymphocyte clusters in lymph nodes: A possible substrate for cellular interactions in the immune response. Am J Anat 144:209–232
Kamperdijk EWA, Raaymakers EM, de Leeuw JHS, Hoefsmit EChM (1978) Lymph node macrophages and reticulum cells in the immune response. I. The primary response to parathyphoid vaccine. Cell Tissue Res 192:1–23
Karnovsky JM (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137A-138A
Luft JH (1961) Improvements in epoxy resin embedding methods. J Biophy Biochem Cytol 9:409–414
Michael SB, Goldstein JL, Krieger M, Ho YK, Anderson RGW (1979) Reversible accumulation of cholesteryl esters in macrophages incubated with acetylated lipoprotein. J Cell Biol 82:597–613
Miyata K, Takaya K (1981) Elastic fibers associated with collagenous fibrils surrounded by reticular cells in lymph nodes of the rat as revealed by electron microscopy after orcein staining. Cell Tissue Res 220:445–448
Mori Y, Lennert K (1969) Electron microscopic atlas of lymph node cytology and pathology. Springer-Verlag, Berlin Heidelberg New York, p 10
Nopajaroonsri C, Luck SC, Simon GT (1971) Ultrastructure of the normal lymph node. Am J Pathol 65:1–24
Nossal GJV, Abbot A, Mitchell J (1968) Antigen in immunity. XIV Electron microscopic radioautographic studies of antigen capture in the lymph node medulla. J Exp Med 127:263–276
Ohkuma S, Poole B (1981) Cytoplasmic vacuolation of mouse peritoneal macrophages and the uptake into lysosomes of weakly basic substances. J Cell Biol 90:656–664
Poole B, Ohkuma S (1981) Effect of weak bases on the intralysosomal pH in mouse peritoneal macrophages. J Cell Biol 90:665–669
Rhodin JAG (1974) Histology. A text and atlas. Oxford University Press, New York London Tronto, p 385
Roberts BV (1969) The effects of steroid hormones on macrophage activity. Int Rev Cytology 25:131–155
Sorensen GD (1960) An electron microscopic study of popliteal lymph nodes from rabbits. Am J Anat 107:73–96
William BP (1978) The mechanism of glucocorticoid effects in fibroblast. J Invest Dermatol 71:24–35
Zena W (1978) Biochemical actions of glucocorticoids on macrophages in culture. Specific inhibition of elastase, collagenase, and plasminogen. J Exp Med 147:1695–1712
Zena W, Rosemary F, Allan M (1978) Interaction of glucocorticoids with macrophages. Identification of glucocorticoid receptors in monocytes and macrophages. J Exp Med 147:1684–1694
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Miyata, K., Takaya, K. Vacuoles in macrophages and reticular cells of regional lymph nodes of the rat after injection of large doses of steroids. Cell Tissue Res. 230, 57–65 (1983). https://doi.org/10.1007/BF00216027
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DOI: https://doi.org/10.1007/BF00216027