Abstract
The human and chimpanzee T-cell receptor gamma-delta (TCR γδ) bearing cells represent a minor subset (3–8%) of T lymphocytes. In the periphery, the TCR γδ population has a restricted combinatorial repertoire. The TCRD-V1 and-V2 gene products are expressed in a mutually exclusive fashion, whereas, the TCRD-V2 and the TCRG-V9 encoded proteins show, in general, a coordinated expression. Restriction fragment length polymorphism analysis showed conservation of the restriction sites that identify the TCRG-V9 and TCRD-V2 rearrangements. The human TCRG-V9 locus has two alleles, TCRG-V9A1 and TCRG-V9A2 differing at codon position 31. The chimpanzee TCRG-V9 gene product differs from the products of the human TCRG-V9A1 and TCRG-V9A2 allele by two and three amino acid replacements, respectively. The human and the chimpanzee TCRG-V9-TCRD-V2 lymphocytes show a similar specific proliferative and cytolytic response to human Daudi Burkitt's lymphoma cells. Therefore, the amino acid replacements found in the chimpanzee TCRG-V9 gene product do not change the superantigen specificity across this species barrier.
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This nucleotide sequence data reported in this paper have been submitted to the EMBL data library and have been assigned the accession number: X61069 P. Troglodytes TCR VGG.
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Sturm, E., Bontrop, R.E., Vreugdenhil, R.J. et al. T-cell receptor gamma/delta: comparison of gene configurations and function between humans and chimpanzees. Immunogenetics 36, 294–301 (1992). https://doi.org/10.1007/BF00215657
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DOI: https://doi.org/10.1007/BF00215657